Point-of-care testing devices with original microfluidic technology and signal amplification method

• Project/Area Number: 22500448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HOSOKAWA Kazuo 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (00373366)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マイクロチップ / イムノアッセイ / ポイント・オブ・ケア / 疾患マーカー / 臨床検査 / 抗体

Research Abstract

We have developed rapid and sensitive detection methods for disease biomarkers using original microchip and amplification technologies. First, we found that covalent immobilization of antibody to the substrate via dextran matrix is advantageous in terms of sensitivity. Next, we succeeded in detecting three biomarker proteins simultaneously on the microchip. Finally, this method was successfully applied to microRNA detection as well.

Research Products

• [Journal Article] Application of microchip phosphate-affinity electrophoresis to measurement of protease activity in complex samples (2013)
  • Author(s): Aishan Han, Kazuo Hosokawa, Mizuo Maeda
  • Journal Title: Analytical Biochemistry Volume: 432 Pages: 8-10
  • Peer Reviewed
• [Journal Article] Hydrodynamic cell pairing and cell fusion through a microslit on a microfluidic device (2012)
  • Author(s): Naoki Sasaki, Jiansheng Gong, Makoto Sakuragi, Kazuo Hosokawa, Mizuo Maeda, Yoshihiro Ito
  • Journal Title: Japanese Journal of Applied Physics Volume: 51 Pages: 30206-30206
  • NAID: 210000140319
  • Peer Reviewed
• [Journal Article] Activity measurement of protein kinase and protein phosphatase by microchip phosphate-affinity electrophoresis (2012)
  • Author(s): Aishan Han, Kazuo Hosokawa, Mizuo Maeda
  • Journal Title: Analytical Biochemistry Volume: 421 Pages: 782-784
  • Peer Reviewed
• [Journal Article] Rapid microRNA detection using power-free microfluidic chip: coaxial stacking effect enhances the sandwich hybridization (2012)
  • Author(s): Hideyuki Arata, Hiroshi Komatsu, Aishan Han, Kazuo Hosokawa, Mizuo Maeda
  • Journal Title: Analyst Volume: 137 Pages: 3234-3237
  • Peer Reviewed
• [Journal Article] Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip (2012)
  • Author(s): Hideyuki Arata, Hiroshi Komatsu, Kazuo Hosokawa, Mizuo Maeda
  • Journal Title: PLOS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip (2012)
  • Author(s): 新田 英之
  • Journal Title: PLOS ONE Volume: 7 Issue: 11 Pages: e48329-e48329
  • DOI: 10.1371/journal.pone.0048329
  • Peer Reviewed
• [Journal Article] Hydrodynamic cell pairing and cell fusion through a microslit on a microfluidic device (2012)
  • Author(s): 佐々木直樹
  • Journal Title: Japanese Journal of Applied Physics Volume: 51 Issue: 3R Pages: 030206-030206
  • DOI: 10.1143/jjap.51.030206
  • Peer Reviewed
• [Journal Article] Power-free microchip immunoassay of PSA in human serum for point-of-care testing (2011)
  • Author(s): Hiroki Okada, Kazuo Hosokawa, Mizuo Maeda
  • Journal Title: Analytical Sciences Volume: 27 Pages: 237-241
  • NAID: 10027906020
  • Peer Reviewed
• [Journal Article] DNA detection on a power-free microchip with laminar flow-assisted dendritic amplification (2010)
  • Author(s): Kazuo Hosokawa, Takahiro Sato, Yasunobu Sato, Mizuo Maeda
  • Journal Title: Analytical Sciences Volume: 26 Pages: 1053-1057
  • NAID: 10026785643
  • Peer Reviewed
• [Journal Article] Power-free microchip immunoassay of PSA in human serum for point-of-care testing
  • Author(s): 岡田浩樹
  • Journal Title: Analytical Sciences Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Power-free microfluidic device for point-of-care medical tests (2013)
  • Author(s): Kazuo Hosokawa
  • Organizer: CRC International Symposium
  • Place of Presentation: Sapporo, Japan
• [Presentation] Power-free microfluidic device for point-of-care medical tests (2013)
  • Author(s): 細川 和生
  • Organizer: CRC International Symposium
  • Place of Presentation: 札幌
  • Invited
• [Presentation] 自律駆動マイクロチップを用いたイムノアッセイ (2012)
  • Author(s): 細川和生
  • Organizer: 生物化学的測定研究会第17回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2012-06-08
• [Presentation] Sub-attomole detection of microRNA in twenty minutes using power-free microfluidic chip (2012)
  • Author(s): Hideyuki Arata, Hiroshi Komatsu, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: towards point-of-care testing, μTAS 2012
  • Place of Presentation: Ginowan, Japan
• [Presentation] Manipulation of individual live cells by micro-fabricated structure (2012)
  • Author(s): Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
  • Organizer: The 7th Sweden-Japan BioNano Workshop
  • Place of Presentation: Stockholm, Sweden
• [Presentation] MicroRNA detection on a power-free microchip toward point-of-care testing (2012)
  • Author(s): Ryo Ishihara, Hideyuki Arata, Hiroshi Komatsu, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: The 7th Sweden-Japan BioNano Workshop
  • Place of Presentation: Stockholm, Sweden
• [Presentation] Sub-attomole detection of microRNA in twenty minutes using power-free microfluidic chip: towards point-of-care testing (2012)
  • Author(s): 新田 英之
  • Organizer: The 16th Micro Total Analysis Systems
  • Place of Presentation: Ginowan, Japan
• [Presentation] Activity measurements of kinases and phosphatases in cell lysates by microchip (2011)
  • Author(s): Aishan Han, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: phosphate-affinity electrophoresis, μTAS 2011
  • Place of Presentation: Seattle, USA
  • Year and Date: 2011-10-03
• [Presentation] Highly sensitive microRNA detection using gold nanoparticles on power-free microfluidic chip (2011)
  • Author(s): Hideyuki Arata, Hiroshi Komatsu, Aishan Han, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: towards point-of-care early-stage cancer diagnosis, μTAS 2011
  • Place of Presentation: Seattle, USA
  • Year and Date: 2011-10-03
• [Presentation] Activity measurements of kinases and phosphatases in cell lysates by microchip phosphate-affinity electrophoresis (2011)
  • Author(s): 韓愛善
  • Organizer: microTAS 2011
  • Place of Presentation: シアトル,米国
  • Year and Date: 2011-10-03
• [Presentation] Highly sensitive microRNAtection using gold nanoparticles on power-free microfluidic chip : towards point-of-care early-stage cancer diagnosis (2011)
  • Author(s): 新田英之
  • Organizer: microTAS 2011
  • Place of Presentation: シアトル,米国
  • Year and Date: 2011-10-03
• [Presentation] 自律駆動マイクロ流体チップと金ナノ粒子によるマイクロRNAの検出 (2011)
  • Author(s): 小松仁,新田英之,韓愛善,細川和生,前田瑞夫
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] マイクロチップ・リン酸基アフィニティー電気泳動法によるチロシンキナーゼc-Srcの活性測定 (2011)
  • Author(s): 臼田雪子,韓愛善,佐藤香枝,細川和生,前田瑞夫
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 日本分析化学会第60年会
  • Year and Date: 2011-09-14
• [Presentation] 自律駆動マイクロチップによる複数の疾患マーカータンパク質同時イムノアッセイ (2011)
  • Author(s): 細川和生,岡田浩樹,前田瑞夫
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] マイクロチップにおけるリン酸基特異的なアフィニティー電気泳動によるキナーゼ/フォスファターゼ活性の測定 (2011)
  • Author(s): 韓愛善,細川和生,前田瑞夫
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] マイクロ流体チップを用いたマウス胸腺由来細胞OP9Gとマウス胚性幹細胞EB3の一対一配置と融合 (2011)
  • Author(s): 森崎良,細川和生,櫻木誠,吉田泰彦,伊藤嘉浩,前田瑞夫
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] マイクロチップ・リン酸基アフィニティー電気泳動法によるチロシンキナーゼc-Srcの活性測定 (2011)
  • Author(s): 臼田雪子
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] 自律駆動マイクロ流体チップを用いたマイクロRNA高感度検出 (2011)
  • Author(s): 小松仁,新田英之,韓愛善,細川和生,前田瑞夫
  • Organizer: 第23回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 千葉
  • Year and Date: 2011-06-11
• [Presentation] Alternating current cloud point extraction on a microfluidic chip for preconcentration of membrane-associated biomolecules (2010)
  • Author(s): Naoki Sasaki, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] Detection of clinical cut-off values of prostate specific antigen in poly(dimethylsiloxane) microchannels for point-of-care testing (2010)
  • Author(s): Hiroki Okada, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] Detection of kinase activity by phosphate-affinity electrophoresis on a PDMS microchip (2010)
  • Author(s): Aishan Han, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] Alternating current cloud point extraction on a microfluidic chip for preconcentration of membrane-associated biomolecules (2010)
  • Author(s): 佐々木直樹
  • Organizer: Pacifichem 2010
  • Place of Presentation: ホノルル, 米国
  • Year and Date: 2010-12-16
• [Presentation] Detection of clinical cut-off values of prostate specific antigen in poly (dimethylsiloxane) microchannels for point-of-care testing (2010)
  • Author(s): 岡田浩樹
  • Organizer: Pacifichem 2010
  • Place of Presentation: ホノルル, 米国
  • Year and Date: 2010-12-16
• [Presentation] Detection of kinase activity by phosphate-affinity electrophoresis on a PDMS microchip (2010)
  • Author(s): 韓愛善
  • Organizer: Pacifichem 2010
  • Place of Presentation: ホノルル, 米国
  • Year and Date: 2010-12-16
• [Presentation] Power-freeマイクロチップを使ったイノムアッセイと医療診断への展開 (2010)
  • Author(s): 岡田浩樹,細川和生,前田瑞夫
  • Organizer: 第22回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-18
• [Presentation] 流れを利用する異種細胞配置・融合用マイクロ流路のアレイ化 (2010)
  • Author(s): 佐々木直樹,森崎良,Jiansheng Gong,細川和生,伊藤嘉浩,前田瑞夫
  • Organizer: 第22回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-18
• [Presentation] 自律駆動マイクロチップによる簡便・高感度なイノムアッセイ (2010)
  • Author(s): 細川和生
  • Organizer: 第30回キャピラリー電気泳動シンポジウム
  • Place of Presentation: 岐阜
  • Year and Date: 2010-11-16
• [Presentation] 自動駆動マイクロチップによる簡便・高感度なイノムアッセイ (2010)
  • Author(s): 細川和生
  • Organizer: 第30回キャピラリー電気泳動シンポジウム
  • Place of Presentation: 岐阜(招待講演)
  • Year and Date: 2010-11-16
• [Presentation] Pairing and fusion of heterotypic cells in a microchannel (2010)
  • Author(s): Naoki Sasaki, Jiansheng Gong, Kazuo Hosokawa, Mizuo Maeda, Yoshihiro Ito
  • Organizer: μTAS 2010
  • Place of Presentation: Groningen, The Netherlands
  • Year and Date: 2010-10-06
• [Presentation] Pairing and fusion of heterotypic cells in a microchannel (2010)
  • Author(s): 佐々木直樹
  • Organizer: micro TAS 2010
  • Place of Presentation: グローニンゲン, オランダ
  • Year and Date: 2010-10-06
• [Presentation] Low-femtomolar detection of biomarker protein by point-of-care immunoassay on a power-free microchip with covalently immobilized antibody (2010)
  • Author(s): Hiroki Okada, Kazuo Hosokawa, Mizuo Maeda
  • Organizer: μTAS 2010
  • Place of Presentation: Groningen, The Netherlands
  • Year and Date: 2010-10-05
• [Presentation] Low-femtomolar detection of biomarker protein by point-of-care immunoassay on a power-free microchip with covalently immobilized antibody (2010)
  • Author(s): 岡田浩樹
  • Organizer: micro TAS 2010
  • Place of Presentation: グローニンゲン, オランダ
  • Year and Date: 2010-10-05
• [Presentation] 細胞融合実験用マイクロ流体チップの開発 (2010)
  • Author(s): 森崎良,佐々木直樹,櫻木誠,細川和生,吉田泰彦,伊藤嘉浩,前田瑞夫
  • Organizer: 日本化学会第4回関東支部大会
  • Place of Presentation: つくば
  • Year and Date: 2010-08-30
• [Presentation] マイクロ流路内異種細胞配置・融合法の開発 (2010)
  • Author(s): 佐々木直樹,Jiansheng Gong, 細川和生,前田瑞夫,伊藤嘉浩
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-11
• [Presentation] マイクロチップイムノアッセイによる前立腺がんマーカーの迅速・簡便・高感度解析 (2010)
  • Author(s): 岡田浩樹,細川和生,前田瑞夫
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-11
• [Remarks]
  • URL: http://www.riken.jp/lab-www/bioengineering/index.htm