| Project/Area Number |
22500468
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Ibaraki Prefectural University of Health Science |
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Principal Investigator |
TOMITA Kazuhide 茨城県立医療大学, 理学療法学科, 教授 (00389793)
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| Co-Investigator(Kenkyū-buntansha) |
IIZUKA Makito 茨城県立医療大学, 医科学センター, 助教 (40274980)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 呼吸筋強化トレーニング / 延髄呼吸ニューロン / c-Fos 免疫組織化学法 / 呼吸生理学的解析 / 電気生理学的解析 / c-Fos免疫組織化学法 |
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| Research Abstract |
Respiratory muscle training (RMT) can improve respiratory muscleweakness in chronic respiratory diseases. However , it is unclear whether RMT inducesadaptations in the respiratory center as well as respiratory muscles. We hypothesized therespiratory center in the medulla would also adapt to the RMT and less neurons became torecruit to achieve same minute ventilation under hypercapnic stimulation. T o clarify thishypothesis, the number of c-Fos positive medulllary neurons after short term hypercapnicstimulation (7%CO2, 50%O2, N2 balance, 90 min) in the rats with RMT was compared tothat in the untrained control rats. For the respiratory muscle training program, we usedthe hypercapnic stimulation that consisted of placing each rat in a hypercapnic chamberin order to increase the minute ventilation. The RMT rats (n=2) were placed in a chamberfilled with 7% CO2 and 50% O2 for 30 min, 5 times a week, for 4 weeks, whereas controlrats (n=2) were placed in the same chamber filled with room air . After 1.5 hours from thecompletion of the last trial of the 4 weeks program, axial sections from the medullaoblongata were processed for c-Fos immunohistochemistry .It was found that the number of the c-Fos positive neurons in the RMT rats were smallerthan those in the control rats at ventral and dorsal region of the medulla oblongata.
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