| Project/Area Number |
22500491
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kibi International University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRAGAMI Fukumi 吉備国際大学, 保健医療福祉学部, 教授 (60278976)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | リハビリテーション / ストレッチ / ストレッチ刺激 / C2C12細胞株 / Myogenin / Myosin / 伸展刺激 / H9c2 cells / MyoD / Myf5 |
|---|
| Research Abstract |
We performed 15-minute relatively short extension stimulation in cultured myoblast (H9c2 cell strain, C2C12 cell strain) mechanically and checked the influence of proliferation and differentiation. In addition, we assayed the expression of the RNAs of MyoD family (MyoD, Myogenin, Myf5, MRF4) with the RT-PCR and Myogenin protein and Myosin protein expression with the Western Blotting method. As a result, stretch stimulation did not adversely affect the proliferation in the skeletal muscle reproduction process, and exerting a positive effect on differentiation was revealed. It was suggested that MyoD RNA , Myogenin RNA and Myf5 RNA and Myogenin protein and Myosin protein played an important role for this.
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