| Project/Area Number |
22500754
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|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
|
|---|
| Research Institution | Toyo University |
|---|
Principal Investigator |
YANO Tomohiro 東洋大学, 生命科学部, 教授 (50239828)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YANO Yoshihisa 京都学園大学, バイオ環境学部, 准教授 (20230287)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | 大豆由来成分 / BBI / 生体利用性 / ELISA法 |
|---|
| Research Abstract |
In order to evaluate bioavailability of Bowman-birk protease inhibitor (BBI), a major anticancer agent involved in soybeans, we tried to establish a new ELISA system which can selectively BBI level in vivo with a high sensitivity. We established a new ELISA system for BBI detection, using monoclonal and polyclonal antibodies towards BBI. To improve detection sensitivity in the system, we changed several points in the system such as determination of blocking agent. As a result, the system had equal or higher detection sensitivity compared to other established protein detectionELISA systems, indicating that this system could detect BBI level in vivo.
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