| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Research Abstract |
In this study, we have mainly developed some experiments for demonstration of difficulty of the preservation of living cells and for demonstration of each protein molecule having specific function. First, as for the experimental teaching materials which can demonstrate difficulty of the cryopreservation of the living things, we examined the condition of thawing as well as freezing of salmon and quail eggs. After freezing and thawing, the eggs which soaked in isosmotic buffer solution ruptured, whereas the majority of the eggs which soaked in the glycerol- containing isotonic buffer solution did not rupture. In addition, we tested the conditions of thawing as well as freezing of mouse macrophages as the living cells. After freeze and thaw treatment, the many macrophages in culture medium ruptured, whereas the majority of the macrophages in the special medium for cell cryopreservation did not rupture and attached to the dish. Since the vitality of the macrophages can be grasped frm the state of their attachment to dish, these combined experiments seem to be adequate for the demonstration of difficulty of the cryopreservation of cells. Secondly, with regard to the experimental teaching materials for demonstration of each protein possessing specific function, we improved the method of gel electrophoresis by using wide sized slide glasses. And then, we utilized it for zymography which is detectable some kinds of enzymes at the same time. Some other popular experimental teaching materials, such as DNA visualization and so on, were also improved or reconsidered in this study.
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