Elucidation of genomic distribution dynamics of DNA damage and repair in carcinogenesis process

• Project/Area Number: 22500997
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Carcinogenesis
• Research Institution: Nagoya University
• Principal Investigator: AKATSUKA Shinya 名古屋大学, 医学系研究科, 講師 (40437223)
• Co-Investigator(Kenkyū-buntansha): TOYOKUNI Shinya 名古屋大学, 大学院・医学系研究科, 教授 (90252460)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 実験動物モデル / ゲノム損傷 / 酸化ストレス / 発がん

Research Abstract

We developed a novel method to detectgenomic sites harboring DNA damage comprehensively and verified practical effectiveness of the method. We conducted immunoprecipitations with genomic DNA extracted from target cells of ferric nitrilotriacetate-induced rodent renal carcinogenesis model using antibodies against two types of representative oxidative modified bases, 8-OHdG and acrolein-dA, and analyzed genomic distributions of the oxidative DNA damages by the use of array-CGH.

Research Products

• [Journal Article] Genome-wide assessment of oxidatively generated DNA damage (2012)
  • Author(s): Akatsuka S, Toyokuni S
  • Journal Title: Free Radic Res Volume: vol.46 Pages: 523-530
  • Peer Reviewed
• [Journal Article] Fenton reaction induced cancer in wild type rats recapitulates genomic alterations observed in human cancer. (2012)
  • Author(s): Akatsuka S, Yamashita Y, Ohara H, Liu YT, Izumiya M, Abe K, Ochiai M, Jiang L, Nagai H, Okazaki Y, Murakami H, Sekido Y, Arai E, Kanai Y, Hino O, Takahashi T, Nakagama H, Toyokuni S
  • Journal Title: PLoS One Volume: vol.7
  • Peer Reviewed
• [Journal Article] 酸化ストレスによるゲノム変化と発がん (2012)
  • Author(s): 赤塚慎也,豊國伸哉
  • Journal Title: 実験医学 Volume: vol.30 Pages: 2779-2785
• [Journal Article] Genome-wide assessment of oxidatively generated DNA damage (2012)
  • Author(s): Akatsuka S
  • Journal Title: Free Radic Res Volume: 46 Issue: 4 Pages: 523-530
  • DOI: 10.3109/10715762.2011.633212
  • Peer Reviewed
• [Journal Article] Fenton Reaction Induced Cancer in Wild Type Rats Recapitulates Genomic Alterations Observed in Human Cancer (2012)
  • Author(s): Liu Y, Izumiya M, Abe K, Ochiai M, Jiang L, Nagai H, Okazaki Y, Murakami H, Sekido Y, Arai E, Kanai Y, Hino O, Takahashi T, Nakagama H, Toyokuni S
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e43403-e43403
  • DOI: 10.1371/journal.pone.0043403
  • URL: https://localhost/en/publications/8d3bdaa1-070a-4972-864d-534f26924cb6
  • Peer Reviewed
• [Journal Article] 酸化ストレスによるゲノム変化と発がん (2012)
  • Author(s): 赤塚 慎也
  • Journal Title: 実験医学 Volume: 30 Pages: 2779-2785
• [Journal Article] Stage-specific roles of fibulin-5 during oxidative stress-induced renal carcinogenesis in rats (2011)
  • Author(s): Ohara H, Akatsuka S, Nagai H, Liu YT, Jiang L, Okazaki Y, Yamashita Y, Nakamura T, Toyokuni S
  • Journal Title: Free Radic Res Volume: vol.45 Pages: 211-220
  • Peer Reviewed
• [Journal Article] Genome-wide assessment of oxidatively generated DNA damage (2011)
  • Author(s): Akatsuka S, Toyokuni S
  • Journal Title: Free Radical Research Volume: 46 Pages: 523-530
  • Peer Reviewed
• [Journal Article] Toyokuni S. Stage-specific roles of fibulin-5 during oxidative stress-induced renal carcinogenesis in rats. (2011)
  • Author(s): Ohara H, et. al.
  • Journal Title: Free Radical Research Volume: 45 Pages: 211-220
  • Peer Reviewed
• [Journal Article] Genome-scale approaches to investigate oxidative DNA damage (2010)
  • Author(s): Akatsuka S, Toyokuni S
  • Journal Title: J Clin Biochem Nutr Volume: vol.47 Pages: 91-97
  • NAID: 130004879210
  • Peer Reviewed
• [Journal Article] A quantitative trait locus responsible for inducing B-cell lymphoblastic lymphoma is a hotspot for microsatellite instability. (2010)
  • Author(s): Kaszynski RH, et. al.
  • Journal Title: Cancer Science Volume: 101 Pages: 800-805
  • Peer Reviewed
• [Journal Article] Genome-scale approaches to investigate oxidative DNA damage. (2010)
  • Author(s): Akatsuka S, Toyokuni S.
  • Journal Title: Journal of Chnical Biochemistry and Nutrition Volume: 47 Pages: 91-97
  • NAID: 130004879210
  • Peer Reviewed
• [Presentation] マウス腎発がん過程における酸化的 DNA 損傷のゲノム内分布 (2012)
  • Author(s): 赤塚慎也
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル[新宿区]
• [Presentation] マウス腎発がん過程における酸化的 DNA 損傷のゲノム内分布 (2012)
  • Author(s): 赤塚慎也
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: あわぎんホール[徳島市]
• [Presentation] マウス腎発がん過程における酸化的 DNA 損傷のゲノム内分布 (2012)
  • Author(s): 赤塚慎也
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌[札幌市]
• [Presentation] マウス腎発がん過程における酸化的 DNA 損傷のゲノム内分布 (2012)
  • Author(s): 赤塚慎也
  • Organizer: フォーラム2012:衛生薬学・環境トキシコロジ-
  • Place of Presentation: 名古屋観光ホテル[名古屋市]).
• [Presentation] マウス腎発がん過程における酸化的DNA損傷のゲノム内分布 (2012)
  • Author(s): 赤塚 慎也
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル[新宿区]
• [Presentation] マウス腎発がん過程における酸化的DNA損傷のゲノム内分布 (2012)
  • Author(s): 赤塚 慎也
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: あわぎんホール[徳島市]
• [Presentation] マウス腎発がん過程における酸化的DNA損傷のゲノム内分布 (2012)
  • Author(s): 赤塚 慎也
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌[札幌市]
• [Presentation] マウス腎発がん過程における酸化的DNA損傷のゲノム内分布 (2012)
  • Author(s): 赤塚 慎也
  • Organizer: フォーラム2012：衛生薬学・環境トキシコロジ―
  • Place of Presentation: 名古屋観光ホテル[名古屋市]
• [Presentation] 鉄ニトリロ三酢酸誘発腎癌モデルを用いた酸化ストレス発がん機構の解析 (2011)
  • Author(s): 赤塚慎也
  • Organizer: 第26回発癌病理研究会
  • Place of Presentation: ルネッサンスサッポロホテル[札幌市]
  • Year and Date: 2011-08-29
• [Presentation] 鉄ニトリロ三酢酸誘発腎癌モデルを用いた酸化ストレス発がん機構の解析 (2011)
  • Author(s): 赤塚慎也
  • Organizer: 第26回発癌病理研究会
  • Place of Presentation: ルネッサンスホテル(札幌)
  • Year and Date: 2011-08-29
• [Presentation] Distribution analysis of restriction enzyme recognition sites for mammalian genomes (2011)
  • Author(s): 向出貴裕
  • Organizer: 2011年日本バイオインフォマティクス学会年会
  • Place of Presentation: 神戸国際会議場(神戸)
• [Presentation] Ferric nitrilotriacetate- induced rat renal cancer exhibits extensive chromosomal instability (2010)
  • Author(s): Akatsuka S
  • Organizer: 15th Japan-Korea Cancer Research Workshop
  • Place of Presentation: シェラトン仁川ホテル[仁川,韓国])
  • Year and Date: 2010-12-22
• [Presentation] Ferric nitrilotriacetate-induced rat renal cancer exhibits extensive chromosomal instability. (2010)
  • Author(s): Akatsuka S
  • Organizer: 15th Japan-Korea Cancer Research Workshop
  • Place of Presentation: シェラトン仁川ホテル(仁川市,韓国)
  • Year and Date: 2010-12-22
• [Presentation] Ferric nitrilotriacetate- induced rat renal cancer exhibits extensive chromosomal instability (2010)
  • Author(s): Akatsuka S
  • Organizer: 8th International Workshop on Genetic Systems in the Rat
  • Place of Presentation: 京都大学国際交流ホール[京都市]
  • Year and Date: 2010-12-03
• [Presentation] Ferric nitrilotriacetate-induced rat renal cancer exhibits extensive chromosomal instability. (2010)
  • Author(s): Akatsuka S
  • Organizer: 8th International Workshop on Genetic Systems in the Rat
  • Place of Presentation: 京都大学国際交流ホール(京都市)
  • Year and Date: 2010-12-03