| Project/Area Number |
22501005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
FUKAMACHI Hiroshi 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (70134450)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | がん細胞の特性 / 幹細胞 / 胃がん / がん幹細胞 / 造腫瘍性 / FACS / ヌードマウス / 無血清培養 / sphere / 細胞表面抗原 / Xenograft / spheroids / ヒト |
|---|
| Research Abstract |
We established xenograft lines where human gastric tumor tissues maintain their differentiation and tumorigenesis. Using these lines, we identified a marker which was specifically expressed by gastric cancer stem cells. We also confirmed that the marker was expressed by cancer stem cells which were obtained from newly-dissected gastric cancers. Moreover, we established serum-free culture system where gastric cancer stem cells proliferate to form spheres, structures specific to stem cells. These sphere-forming cells expressed the antigen and were highly tumorigenic. We are now examining the mechanism how their tumorigenicity is controlled.
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