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Study on functional significance of transcription factor GATA-6during oncogenic signal transduction

Research Project

Project/Area Number 22501007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Tumor biology
Research InstitutionShinshu University

Principal Investigator

ADACHI Yoshifumi  信州大学, 医学部, 准教授 (50201893)

Co-Investigator(Kenkyū-buntansha) KAMATA Tohru  信州大学, 医学部, 教授 (40056304)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords転写因子 / GATA-6 / シグナル伝達 / GATA-6 / TOPORS / TOPORS / Nox1
Research Abstract

We clarified that phosphorylation of GATA-6 and GATA-6-associated cofactor HMGB1 positively regulate the transcriptional machinery for Nox1 gene in oncogenic Ras signaling. We identified 719 genes upregulated byGATA-6 and 1161 genes downregulated by GATA-6 in colon cancer cell-line CaCo-2. We also clarified the molecular mechanism for transcriptional repression of TOPORS gene by GATA-6.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (11 results)

  • [Journal Article] TA strategy for site-directed mutagenesis2012

    • Author(s)
      Adachi Y., Fukuhara C.
    • Journal Title

      Anal. Biochem

      Volume: Vol.431 Pages: 66-68

    • URL

      http://dx.DOI:.org/10.1016/j.ab.2012.08.030

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acrucial role for Nox 1 in redox-dependent regulation of Wnt-b-catenin signaling.2012

    • Author(s)
      Kajla S., Mondol AS., Nagasawa A., Zhang Y., Kato M., Matsuno K., Yabe-Nishimura C., Kamata T.
    • Volume
      Vol .26、No.5
    • Pages
      2049-2059
    • URL

      http://www.fasebj.org/content/26/5/2049.long

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] TA strategy for rapid and efficient site-directed mutagenesis2012

    • Author(s)
      Yoshifumi Adachi
    • Journal Title

      Analytical Biochemistry

      Volume: 431 Pages: 66-68

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reactive oxygen generated by NADPH oxidase 1 (Nox1) contributes to cell invasion by regulating matrix metalloprotease-9 production and cell migration.2010

    • Author(s)
      Shinohara M., Adachi Y., Mitsushita J., Kuwabara M., Nagasawa A., Harada S., Furuta S., Zhang Y., Seheli K., Miyazaki H., Kamata T
    • Volume
      Vol .285、No.7
    • Pages
      4481-4488
    • NAID

      120007110409

    • URL

      http://www.jbc.org/content/285/7/4481

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reactive Oxygen Generated by NADPH Oxidase 1(Nox1)Contributes to Cell Invasion by Regulating Matrix Metalloprotease-9 Production and Cell Migration.2010

    • Author(s)
      Shinohara Masahiro
    • Journal Title

      J.Biol. Chem.

      Volume: 285 Pages: 4481-4488

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] High mobility group protein- B1 (HMGB1) is a novel activator of GATA-6 in the transcriptional machinery for NADPH oxidase1.2012

    • Author(s)
      安達喜文
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Transcriptional repression of TOPORS gene expression by GATA-6.2012

    • Author(s)
      安達喜文
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 発癌のシグナル伝達におけるTOPORS の機能的役割:Functional characterization of GATA-6-regulated TOPORS in oncogenic signal transduction2011

    • Author(s)
      安達喜文
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2011-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 発癌のシグナル伝達におけるTOPOSの機能的役割 : Functional characterization of GATA-6-regulated TOPORS in oncogenic signal transduction2011

    • Author(s)
      安達喜文
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulatory mechanism of Ras-ERK signaling-responsive transcript- tion factor GATA-6 that mediates the expression of NADPH oxidase (Nox) 12010

    • Author(s)
      安達喜文
    • Organizer
      第83回日本生化学会・第33回に本分子生物学会年会合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulatory mechanism of Ras-ERK signaling-responsive transcription factor GATA-6 that mediates the expression of NADPH oxidase(Nox)1.2010

    • Author(s)
      安達喜文
    • Organizer
      第83回日本生化学会・第33回日本分子生物学会年会合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulation of the NADPH oxidase 1 gene expression by the GATA-62010

    • Author(s)
      安達喜文
    • Organizer
      第60回藤原セミナー国際シンポジウ「Zinc signaling and cellular functions」
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2010-10-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation of the NADPH oxidase 1 gene expression by the GATA-6.2010

    • Author(s)
      安達喜文
    • Organizer
      第60回藤原セミナー国際シンポジウム「Zinc signaling and cellular functions」
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ras/ERKシグナルによるGATA-6のリン酸化とNADPHオキシダーゼ1(Nox1)遺伝子発現の制御機構2010

    • Author(s)
      安達喜文
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2010-09-22
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Transcriptional repression of TOPORS gene expression by GATA-6.

    • Author(s)
      安達喜文
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] High mobility group protein-B1 (HMGB1) is a novel activator of GATA-6 in the transcriptional machinery for NADPH oxidase 1.

    • Author(s)
      安達喜文
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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