Identification of the genes regulate Ras-mediated signalingby cDNA expression cloning method

• Project/Area Number: 22501010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kyoto University
• Principal Investigator: KITAYAMA Hitoshi 京都大学, 医学研究科, 准教授 (30231286)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: がん制御遺伝子

Research Abstract

It is important to regulate Ras-mediated signaling since constitutively active mutations of Ras genes are frequently found in various kinds of tumor cells. In this project we are aiming to identify genes with transformation suppressor activity against DT cells harboring constitutively active Ras oncogene. After a series of cDNA expression cloning around 80 candidate genes were identified.

Research Products

• [Journal Article] The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs (2012)
  • Author(s): Yamamoto M, Matsuzaki T, Takahashi R, Adachi E, Maeda Y, Yamaguchi S, Kitayama H, Echizenya M, Morioka Y, Alexander DB, Yagi T, Itohara S, Nakamura T, Akiyama H, Noda M
  • Journal Title: Biology Open Volume: (in press) Issue: 5 Pages: 458-466
  • DOI: 10.1242/bio.2012638
  • Peer Reviewed
• [Journal Article] A novel screen using the Reck tumor suppressor gene promoter detects both conventional and metastasis-suppressing anticancer drugs (2010)
  • Author(s): Murai R, Yoshida Y, Muraguchi T, Nishimoto E, Morioka Y, Kitayama H, Kondoh S, Kawazoe Y, Hiraoka M, Uesugi M, Noda M
  • Journal Title: Oncotarget Volume: 1 Pages: 252-64
  • URL: http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=136
  • Peer Reviewed
• [Journal Article] Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice (2010)
  • Author(s): Chandana P. S. E、Maeda Y、Ueda A、Kiyonari H、Oshima N、Yamamoto M、Kondo S、Oh J、Takahashi R、Yoshida Y、Kawashima S、Alexander. B. D、Kitayama H、Takahashi C、Tabata Y、Matsuzaki T、Noda M
  • Journal Title: BMC Developmental Biology Volume: Vol.10、No.84 Issue: 1 Pages: 1-13
  • DOI: 10.1186/1471-213x-10-84
  • NAID: 120002511238
  • Peer Reviewed
• [Journal Article] What we learn from transformation suppressor genes: lessons from RECK (2010)
  • Author(s): Noda M, Takahashi C, Matsuzaki T, Kitayama H
  • Journal Title: Future Oncol. Volume: 6 Issue: 7 Pages: 1105-16
  • DOI: 10.2217/fon.10.80
  • Peer Reviewed
• [Journal Article] Noda MHypoxia and RAS-signaling pathways converge on, and cooperatively downregulate, the RECK tumor-suppressor protein through microRNAs. (2010)
  • Author(s): Loayza-Puch F, Yoshida Y, Matsuzaki T, Takahashi C, Kitayama H
  • Journal Title: Oncogene Volume: 29 Issue: 18 Pages: 2638-48
  • DOI: 10.1038/onc.2010.23
  • Peer Reviewed
• [Journal Article] What we learn from transformation suppressor genes : lessons from RECK. (2010)
  • Author(s): Noda, M., Takahashi, C., Matsuzaki, T., Kitayama, H
  • Journal Title: Future Oncology Volume: 6 Pages: 1105-1116
  • Peer Reviewed
• [Presentation] cDNA 発現クローニング法を用いたRas制御因子の探索 (2012)
  • Author(s): 清水正樹、施恭平、野田亮、北山仁志
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-13
• [Presentation] cDNA発現クローニング法を用いたRas制御因子の探索 (2012)
  • Author(s): 清水正樹、施恭平、野田亮、北山仁志
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場