The role of dynamin in actin dynamics: Development of anticancer drugs inhibiting dynamin function
| Project/Area Number |
22501013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Okayama University |
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Principal Investigator |
ABE Tadashi 岡山大学, 医学部, 技術専門職員 (60423282)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80325092)
WATANABE Masami 岡山大学, 岡山大学病院, 准教授 (70444677)
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| Co-Investigator(Renkei-kenkyūsha) |
ASAI Akira 静岡県立大学, 大学院・創薬探索センター, 教授 (60381737)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ダイナミン / がん / 浸潤 / 遊走 / dynasore / アクチン |
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| Research Abstract |
In search of more effective inhibitor for dynamin than dynasore, one hundred eighty one dynasore analogues were screened by in vitro actin polymerization assay. N'-(4-(diethylamino)benzylidene)-4-methoxybenzohydrazide (DBHA) was identified as a potent dynamin inhibitor. DBHA strongly inhibited the serum-stimulated ruffle formation, cell migration and invasion. Under these conditions, DBHA showed little toxicity for cultured cancer cell line. Furthermore, we clarified the function of dynamin/cortactin complex in the regulation of actin dynamics. The dynamin/cortactin complexes bundled several actin filaments and the bundling stabilized actin filaments. The actin bundling by dynamin/cortactin complex was necessary for formation of growth cone filopodia and cell migration. These findings might be crucial for developping anti cancer drugs.
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Report
(4 results)
Research Products
(24 results)
