Identification and analysis of novel cancer-cooperating factors using non-biased genome-wide approach
Project/Area Number |
22501020
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Toho University (2012) The Institute of Physical and Chemical Research (2010-2011) |
Principal Investigator |
NAITO Taku 東邦大学, 医学部, 講師 (10568728)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | T 細胞分化 / リンパ腫 / エピジェネティクス / 細胞増殖 / T細胞分化 / Ikaros / クロマチン / ヒストン修飾 / Mi-2β / Notchl / ガン抑制 |
Research Abstract |
Mutation of transcription factor causes T-cell lymphoma both in human and mouse. To reveal the molecular mechanism of lymphoma suppression by Ikaros, I determined the gene expression profile, and genome-wide distribution of Ikaros and various histone modifications. A number of Ikaros-target genes were found to have high level of H3K27 tri-methylation (H3K27me3), which abolish in Ikaros knockout cells. T-cell specific erasure of H3K27me3 by Eedknockout resulted in hyper-proliferation of T cells upon mitotic stimulation. Thus, it was strongly suggested that decrease of H3K27me3 at TCR-downstream genes is part of the cause of hyper-proliferation and lymphomagenesis in Ikaros mutant.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Harnessing of the Nucleosome Remodeling Deacetylase complex is required for lymphocyte development2012
Author(s)
Zhang J*, Jackson AF*, Naito T*, Seavitt JR, Liu F, Dose F, Kashiwagi M, Yoshida T, Gounari F, Petrie H, Georgopoulos K
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Journal Title
Nature Immunol
Volume: 13
Issue: 1
Pages: 86-94
DOI
Related Report
Peer Reviewed
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[Journal Article] Alternative promoter usage at the Notch1 locus supports ligand-independent signaling in T cell development and leukemogenesis2010
Author(s)
Gomez-del Arco, P., Kashiwagi, M.,*, Jackson, A.F.*, Naito, T.*, Zhang, J., Liu, F., Kee, B., Radtke, F., Redondo, J.M., Georgopoulos, K
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Journal Title
Immunity
Volume: 33
Issue: 5
Pages: 685-98
DOI
Related Report
Peer Reviewed
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