| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Mutation of transcription factor causes T-cell lymphoma both in human and mouse. To reveal the molecular mechanism of lymphoma suppression by Ikaros, I determined the gene expression profile, and genome-wide distribution of Ikaros and various histone modifications. A number of Ikaros-target genes were found to have high level of H3K27 tri-methylation (H3K27me3), which abolish in Ikaros knockout cells. T-cell specific erasure of H3K27me3 by Eedknockout resulted in hyper-proliferation of T cells upon mitotic stimulation. Thus, it was strongly suggested that decrease of H3K27me3 at TCR-downstream genes is part of the cause of hyper-proliferation and lymphomagenesis in Ikaros mutant.
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