| Project/Area Number |
22501030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Osaka University |
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Principal Investigator |
KURONO Sadamu 大阪大学, 大学院・医学系研究科, 招へい准教授 (20271554)
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| Co-Investigator(Kenkyū-buntansha) |
HIEDA Miki 大阪大学, 大学院・医学系研究科, 特任助教 (00380254)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUURA Shuji 大阪大学, 大学院・医学系研究科, 特任教授 (90142322)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 腫瘍マーカー / プロテオミクス / 乳癌 / 乳頭分泌液 / 早期乳癌診断 / 簡易診断キット |
|---|
| Research Abstract |
We measured proteome in nipple discharge (ND) and nipple aspirate fluid like (NAF-L), which were collected from breast cancer and non-cancer patients, using two dimensional nano-LC-nanoelectrospray mass spectrometry (ESI-MS), and then 15 kinds of proteins were found as breast cancer candidate markers by various kinds of statistical technique. Furthermore, we have examined the expression of such protein candidate markers using histological stain and ELISA methods for validating as new breast cancer markers.
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