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Investigation of tumor heterogeneity through analyses of local distribution of tissue metabolites using imaging mass spectrometry

Research Project

Project/Area Number 22501034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Tumor diagnosis
Research InstitutionKeio University

Principal Investigator

WAKUI Masatoshi  慶應義塾大学, 医学部, 講師 (90240465)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsがんの個性診断 / 腫瘍組織 / 顕微質量分析 / 代謝物 / 不均一性 / がん / 代謝適応 / in vivoモデル / 臨床検体
Research Abstract

We successfully detected local distribution of tissue metabolites by imaging mass spectrometry to reveal the tumor heterogeneity formed by metabolic adaptation. These results provide insights into the metabolomic aspects of tumor biology, aiding in the de

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010

All Journal Article (18 results) (of which Peer Reviewed: 13 results) Presentation (31 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 2 results)

  • [Journal Article] Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene.2012

    • Author(s)
      Yamazaki H, Chijiwa T, Inoue Y, Abe Y, Suemizu H, Kawai K, Wakui M, Furukawa D, Mukai M, Kuwao S, Saegusa M, Nakamura M.
    • Journal Title

      Exp Ther Med.

      Volume: 3 (5) Pages: 793-796

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene.2012

    • Author(s)
      Yamazaki H
    • Journal Title

      Experimental and Therapeutic Medicine

      Volume: 3 Pages: 793-796

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of imaging mass spectrometry (IMS) dataset extract software, IMS convolution.2011

    • Author(s)
      Hayasaka T, Goto-InoueN, Ushijima M, Yao I, Yuba-Kubo A, Wakui M, Shigeki Kajihara S, Matsuura M, Setou M
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 401 (1) Pages: 183-193

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmunodeficient NOG mice2011

    • Author(s)
      KuboA, Ohmura M,Wakui M, Takeshita K, Harad T, Kajihara S, Ogawa K, SuemizuH, Nakamura M, Setou M, Suematsu M.
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 400 (7) Pages: 1895-1904

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lovastatin and valproic acid additively attenuate cell invasion in ACC-MESO-1 cells.2011

    • Author(s)
      Yamauchi Y, Izumi Y, Asakura K, Fukutomi T, Serizawa A, Kawai K, Wakui M, Suematsu M, Nomori H.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 410 (2) Pages: 328-332

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The cytostatic effects of lovastatin on ACC-MESO-1 cells.2011

    • Author(s)
      Asakura K, Izumi Y, Yamamoto M, Yamauchi Y, Kawai K, Serizawa A, Mizushima, T, Ohmura M, Kawamura M, Wakui M, Adachi T, Nakamura M, Suematsu M, Nomori H.
    • Journal Title

      J. Surg. Res.

      Volume: 170 (2)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The reconstituted 'humanized liver' in TK-NOG mice is mature and functional.2011

    • Author(s)
      Hasegawa M, Kawai K, Mitsui T, Taniguchi K, Monnaoi M, Wakui M, Ito M,Suematsu M, Peltz G, Nakamura M, Suemizu1 M.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 405 (3) Pages: 405-410

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The influence of donor age on liver regeneration and hepatic progenitor cell populations.2011

    • Author(s)
      Ono Y, Kawachi S, Hayashida T, Wakui M, Tanabe M, Itano O, Obara H, Shinoda M, Hibi T, Oshima G, Tani N, Mihara K, Kitagawa Y.
    • Journal Title

      Surgery.

      Volume: 150 (2) Pages: 154-161

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 質量分析によるがんの分子イメージング2011

    • Author(s)
      涌井昌俊、久保亜紀子、大村光代、末松誠
    • Journal Title

      最新医学

      Volume: 66 (10) Pages: 2321-2329

    • Related Report
      2012 Final Research Report
  • [Journal Article] Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmuno deficient NOG mice2011

    • Author(s)
      Kubo A
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 400 Pages: 1895-1904

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of imaging mass spectrometry (IMS) dataset extract software, IMS convolution2011

    • Author(s)
      Hayasaka T
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 401 Pages: 183-193

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 質量分析によるがんの分子イメージング2011

    • Author(s)
      涌井昌俊
    • Journal Title

      最新医学

      Volume: 66 Pages: 2321-2329

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmunodeficient NOG mice.2011

    • Author(s)
      Kubo A
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced CD73 expression and its association with altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases.2010

    • Author(s)
      Matsuyama M, Wakui M, Monnai M, Mizushima T, Nishime C, Kawai K, Ohmura M, Suemizu H, Hishiki T, Suematsu M, Murata M, Chijiwa T, Furukawa D, OgoshiK, Makuuchi H, Nakamura M.
    • Journal Title

      Oncol. Lett.

      Volume: 1 Pages: 431-436

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 生命科学・医学領域における質量分析イメージングの展開:臨床検査への応用の可能性.2010

    • Author(s)
      涌井昌俊、久保亜紀子、末松誠、村田満
    • Journal Title

      日本臨床検査自動化学会会誌

      Volume: 35 (3) Pages: 269-276

    • Related Report
      2012 Final Research Report
  • [Journal Article] NOGマウスを用いたヒト化動物モデルの研究展開.2010

    • Author(s)
      涌井昌俊、末水洋志.
    • Journal Title

      生化学

      Volume: 82 (4) Pages: 314-318

    • NAID

      10026301245

    • Related Report
      2012 Final Research Report
  • [Journal Article] Reduced CD73 expression and its association with altered purine nucleutide metabolism in colorectal cancer cells robustly causing liver metastases.2010

    • Author(s)
      Matsuyama M
    • Journal Title

      Oncology Letters

      Volume: 1 Pages: 431-436

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生命科学・医学領域における質量分析イメージングの展開:臨床検査への応用の可能性2010

    • Author(s)
      涌井昌俊
    • Journal Title

      日本臨床検査自動化学会会誌

      Volume: 35 Pages: 269-276

    • Related Report
      2010 Annual Research Report
  • [Presentation] New software for imaging mass spectrometry data.2012

    • Author(s)
      MatsuuraM、Ushijima M, Yuba-KuboA,WakuiM, Hosaka K, Hayasaka T, Masaki N, Goto-Inoue N, Miyata S, Yao I, Setou M, Ogawa K, Kajihara S.
    • Organizer
      Presented at the 103rd Annual Meeting of American Association for Cancer Research
    • Place of Presentation
      Chicago Illinoi, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] New software for imaging mass spectrometry data.2012

    • Author(s)
      Matsuura M
    • Organizer
      the 103rd Annual Meeting of American Association for Cancer Research
    • Place of Presentation
      シカゴ(アメリカ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 質量顕微鏡とCE-MSを用いたヒト大腸がん肝転移モデルのin vivo flux解析の試み2011

    • Author(s)
      久保亜紀子
    • Organizer
      第36回日本医用マススペクトル学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 質量顕微鏡(MALDI-IT-TOFMS)とCE-MSを用いたヒト大腸ガン肝転移モデルの局所代謝解析2011

    • Author(s)
      久保亜紀子
    • Organizer
      第38回BMSコンファレンス(BMS2011)
    • Place of Presentation
      箱根高原ホテル(神奈川県)
    • Year and Date
      2011-07-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト大腸ガンのIn vivo代謝を質量顕微鏡で観る2011

    • Author(s)
      久保亜紀子
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Semi-quantitative microscopic MALDI imaging for metabolites in human colon cancer-bearing livers of superimmunodeficient NOG mice2011

    • Author(s)
      Kubo A
    • Organizer
      the 59^<th> American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      米国(デンバー)
    • Year and Date
      2011-06-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Software for peak picking using common peak method for imaging mass spectrometry data2011

    • Author(s)
      Ushijima M
    • Organizer
      the 59^<th> American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      米国(デンバー)
    • Year and Date
      2011-06-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Semi-quantitative microscopic MALDI imaging formetabolites in human colon cancer-bearing livers of superimmunodeficient NOG mice.2011

    • Author(s)
      Kubo A, Ohmura M, Hishiki T, Wakui M, Kawai K, Nishime C, Murata M, Setou M, Suematsu M.
    • Organizer
      the 59th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Denver Colorado, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Software for peak picking using common peak method for imaging mass spectrometry data.2011

    • Author(s)
      Ushijima M, Kajihara S, Hosaka K, Hayasaka T, Goto-Inoue N, Yuba-Kubo A, Wakui M, Yao I, Setou M, Matsuura M
    • Organizer
      the 59th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Denver Colorado, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト大腸ガンのIn vivo 代謝を質量顕微鏡で観る2011

    • Author(s)
      久保亜紀子、大村光代、涌井昌俊、小 河 潔、瀬藤光利、末松 誠.
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道
    • Related Report
      2012 Final Research Report
  • [Presentation] 質量顕微鏡(MALDI-IT-TOFMS)とCE-MSを用いたヒト大腸ガン肝転移モデルの局所代謝解析.2011

    • Author(s)
      久保亜紀子、大村光代、涌井昌俊、小 河 潔、瀬藤光利、末松 誠
    • Organizer
      第38回BMSコンファレンス (BMS2011)
    • Place of Presentation
      神奈川県
    • Related Report
      2012 Final Research Report
  • [Presentation] イメージング質量分析に基づいて領域を判別するソフトウェアの開発.2011

    • Author(s)
      牛嶋大、梶原茂樹、保坂蔵人、宮田敏、早坂孝宏、井上菜穂子、正木紀隆、久保亜紀子、涌井昌俊、矢尾育子、小河潔、瀬藤光利、松浦正明
    • Organizer
      第59回日本質量分析学会総合討論会
    • Place of Presentation
      大阪府
    • Related Report
      2012 Final Research Report
  • [Presentation] 質量顕微鏡とCE-MS を用いたヒト大腸がん肝転移モデルのin vivo flux解析の試み.2011

    • Author(s)
      久保亜紀子、大村光代、涌井昌俊、小 河 潔、瀬藤 光利、末松 誠
    • Organizer
      第36回日本医用マススペクトル学会年会
    • Place of Presentation
      大阪府
    • Related Report
      2012 Final Research Report
  • [Presentation] 高分解能質量顕微鏡によるがん転移肝の代謝プロファイルの解明2010

    • Author(s)
      大村光代
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      日本(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Transporlal metastasis of human colon cancer impairs gluconeogenesis in superimmunodeficient NOG mice.2010

    • Author(s)
      Ohmura M
    • Organizer
      The 9th World Congress for Microcirculation
    • Place of Presentation
      フランス(パリ)
    • Year and Date
      2010-09-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高分解能質量顕微鏡を用いたがん転移肝のエネルギー代謝変動の解明2010

    • Author(s)
      大村光代
    • Organizer
      第5回メタボロームシンポジウム
    • Place of Presentation
      日本(鶴岡)
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 質量顕微鏡とCE-MSによる担がん肝の局所代謝解析2010

    • Author(s)
      久保亜紀子
    • Organizer
      第35回日本医用マススペクトル学会年会
    • Place of Presentation
      日本(名古屋)(招待講演)
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microscopic imaging mass spectrometry on hepatic micrometastasis of human colon cancer xenografts in superimmunodeficient NOG mice.2010

    • Author(s)
      Kubo A
    • Organizer
      The 58^<th> American Society for mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      米国(ソルトレイクシティ)
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Metaholnmic profiling of the liver bearing human colon cancer metastasis in superimmunodeficient NOG mice.2010

    • Author(s)
      Ohmura M
    • Organizer
      Experimental Biology 2010
    • Place of Presentation
      米国(アナハイム)
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Reduced ecto-5'-nucleotidase CD73 expression and altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases.2010

    • Author(s)
      Malsuvama M
    • Organizer
      The 100^<st> Annual Meeting of American Associstion for Cancer Research
    • Place of Presentation
      米国(ワシントンD.C.)
    • Year and Date
      2010-04-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Adaptation of cancer cells to hypoxic environments for tumor progression.2010

    • Author(s)
      Mizushima T
    • Organizer
      The 100^<st> Annual Meeting of American Associstion for Cancer Research
    • Place of Presentation
      米国(ワシントンD.C.)
    • Year and Date
      2010-04-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Reduced ecto-5'-nucleotidase CD73 expression and altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases.2010

    • Author(s)
      Matsuyama M, Wakui M, Monnai M, Mizushima T, Nishime C, Kawai K, OhmuraM, Suemizu H, Hishiki T, Suematsu M, Murata M, Chijiwa T, Furukawa D^2, OgoshiK, Makuuchi H, Nakamura M.
    • Organizer
      the 101st Annual Meeting of American Association for Cancer Research
    • Place of Presentation
      Washington, DC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Adaptation of cancer cells to hypoxic environments for tumor progression.2010

    • Author(s)
      Mizushima T, Kawai K, Nishime C, OhmuraM, Hayakawa E, Matsuyama M, Asakura K, Izumi Y, Suemizu H, Ohnishi Y, NakamuraM, Suematsu M, Wakui M.
    • Organizer
      the 101st Annual Meeting of American Association for Cancer Research
    • Place of Presentation
      Washington, DC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Metabolomic profiling of the liver bearing human colon cancer metastasis in superimmunodeficient NOG mice.2010

    • Author(s)
      Ohmura M, Handa K, Nishime C, Mizushima T, Kawai K, Hishiki T, Yamamoto T, Wakui M, Suemizu H, Namakura M, Kiyagawa Y, Suematsu M.
    • Organizer
      Experimental Biology 2010
    • Place of Presentation
      Anaheim, California, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel method to display the structural features included in the mass spectrometryimaging dataset.2010

    • Author(s)
      Yasuno M, Kajihara S, Morinaga H, Ikegami M, Ogawa K, Kubo A, Wakui M, Hayasaka T, Setou M.
    • Organizer
      the 58th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Salt Lake City, Utah, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Microscopic imaging mass spectrometry on hepatic micrometastasis of human colon cancer xenografts in superimmunodeficient NOG mice2010

    • Author(s)
      Kubo A, Ohmura M, Wakui M, Kawai K, Nishime C, Hishiki T, Murata M, Ogawa K, Setou M, Suematsu M
    • Organizer
      the 58th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Salt Lake City, Utah, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Transportal metastasis of human colon cancer impairs gluconeogenesis in superimmunodeficient NOG mice.2010

    • Author(s)
      Ohmura M, Kubo A, Handa K, Nishime C, Mizushima T, Kawai K, Hishiki T, Yamamoto T, Wakui M, Suemizu H, Nakamura M, Kitagawa Y, Setou M, Suematsu M.
    • Organizer
      The 9th World Congress for Microcirculation
    • Place of Presentation
      Paris, France
    • Related Report
      2012 Final Research Report
  • [Presentation] Imaging Mass Spectrometryデータにおける構造解析ソフトの開発2010

    • Author(s)
      森永浩子、梶原茂樹、安野元英、池上将弘、小河潔、久保亜紀子、涌井昌俊、早坂孝宏、瀬藤光利
    • Organizer
      第58回日本質量分析学会総合討論会/第1回アジア・オセアニア質量分析会議
    • Place of Presentation
      茨城県
    • Related Report
      2012 Final Research Report
  • [Presentation] 質量顕微鏡とCE-MSによる担がん肝の局所代謝解析.2010

    • Author(s)
      久保亜紀子、大村光代、涌井昌俊、末松誠
    • Organizer
      第35回日本医用マススペクトル学会年会
    • Place of Presentation
      愛知県
    • Related Report
      2012 Final Research Report
  • [Presentation] 高分解能質量顕微鏡を用いたがん転移肝のエネルギー代謝変動の解明.2010

    • Author(s)
      大村光代、久保亜紀子、菱木貴子、川井健司、涌井昌俊、末水洋志、中村雅登、小河潔、瀬籐光利、末松誠.
    • Organizer
      第5回メタボロームシンポジウム
    • Place of Presentation
      山形県
    • Related Report
      2012 Final Research Report
  • [Presentation] 高分解能質量顕微鏡によるがん転移肝の代謝プロファイルの解明2010

    • Author(s)
      大村光代、久保亜紀子、菱木貴子、川井健司、涌井昌俊、末水洋志、中村雅登、瀬藤光利、末松誠
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      兵庫県
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 質量分析データ処理方法及び装置2012

    • Inventor(s)
      松浦 正明、牛嶋 大、涌井昌俊、瀬藤光利、梶原茂樹、小河 潔
    • Industrial Property Rights Holder
      公益財団法人がん研究会、株式会社島津製作所、国立大学法人浜松医科大学、学校法人慶應義塾
    • Industrial Property Number
      2012-102636
    • Filing Date
      2012-04-27
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Patent(Industrial Property Rights)] MASS-ANALYSYS DATA PROCESSING METHOD AND SYSTEM2012

    • Inventor(s)
      Masaaki Matsuura, Masaru Ushijima, Masatoshi Wakui, Mitsutoshi Setou, Shigeki Kajihara, Kiyoshi Ogawa
    • Industrial Property Rights Holder
      JAPANESE FOUNDATION FOR CANCER RESEARCH, SHIMADZU CORPORATION, National University Corporation Hamamatsu University Of Medicine, KEIO UNIVERSITY
    • Filing Date
      2012-04-27
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] MASS-ANALYSYS DATA PROCESSING METHOD AND SYSTEM2012

    • Inventor(s)
      松浦 正明、牛嶋 大、涌井昌俊、瀬藤光利、梶原茂樹、小河 潔
    • Industrial Property Rights Holder
      公益財団法人がん研究会その他
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-04-27
    • Related Report
      2012 Annual Research Report
    • Overseas

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Published: 2010-08-23   Modified: 2019-07-29  

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