| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Cellular reduction-oxidation (redox) states are regulated mainly by the cysteine thiol groups in protein molecules. Using a fluorescent chemical probe specific with reductive thiol groups, we established reaction conditions for quantitative thiol modifications. Non-reduction/reduction two-dimensional electrophoresis is a promising choice towards comprehensive redox state analysis. Polyacrylamide gel strip backing with a plastic film enabled higher reproducibility of the first dimensional separation. In addition, we made a program of extracting peptide information containingselected post-translational modification(s) from public sequence databases.
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