Identification and validation of biomarker proteins based ontheir thiol redox states
Project/Area Number |
22501035
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Tumor diagnosis
|
Research Institution | Tokyo Medical University |
Principal Investigator |
KAWAKAMI Takao 東京医科大学, 医学部, 客員准教授 (40366117)
|
Co-Investigator(Renkei-kenkyūsha) |
YANO Hiroyuki 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所, ユニット長 (20355580)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 酸化還元 / プロテオーム / 化学修飾 / 質量分析 / 翻訳後修飾 / 配列データベース / 蛋白質データベース / レドックス制御 / バイオマーカー / 酸化修飾蛋白質 / 癌 |
Research Abstract |
Cellular reduction-oxidation (redox) states are regulated mainly by the cysteine thiol groups in protein molecules. Using a fluorescent chemical probe specific with reductive thiol groups, we established reaction conditions for quantitative thiol modifications. Non-reduction/reduction two-dimensional electrophoresis is a promising choice towards comprehensive redox state analysis. Polyacrylamide gel strip backing with a plastic film enabled higher reproducibility of the first dimensional separation. In addition, we made a program of extracting peptide information containingselected post-translational modification(s) from public sequence databases.
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Report
(4 results)
Research Products
(19 results)