Development of cancer diagnostic system using anti-diacetylpolyamine RNA aptamer

• Project/Area Number: 22501036
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: Jikei University School of Medicine
• Principal Investigator: OGURO Akihiro 東京慈恵会医科大学, 医学部, 講師 (00292508)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 機能性RNA / アプタマー / がん診断 / ジアセチルスペルミン / ポリアミン

Research Abstract

Polyamines are ubiquitous biogenic amines and essential for proliferation. Cancer cells generally contain elevated levels of polyamines. Anti-polyamine aptamers would discriminate each polyamine and be potential diagnostic and prognostic tools for malignant disorders. In this study, I isolated RNA aptamer against polyamine and analyzed its binding activity, specificity and polyamine-binding property. Furthermore, I indicated the sensing system with this aptamer was useful to detect polyamine in solution.

Research Products

• [Presentation] RNA アプタマーの解析から明らかにされたスペルミンに結合する RNA 構造 (2012)
  • Author(s): 小黒明広
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-11
• [Presentation] スペルミン結合 RNA アプタマーのバルジ構造の重要性 (2012)
  • Author(s): 小黒明広
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2012-07-18
• [Presentation] RNA アプタマーを用いたスペルミン結合モチーフの解明RNA アプタマーを用いたスペルミン結合モチーフの解明 (2012)
  • Author(s): 小黒明広
  • Organizer: 日本ポリアミン学会 第3回年会
  • Place of Presentation: さいたま市民会館(埼玉県)
  • Year and Date: 2012-01-26
• [Presentation] RNAアプタマーを用いたスペルミン結合モチーフの解明 (2012)
  • Author(s): 小黒明広
  • Organizer: 日本ポリアミン学会第3回年会
  • Place of Presentation: さいたま市民会館(埼玉県)
  • Year and Date: 2012-01-26
• [Presentation] Identification of spermine binding RNA sequence/structure on anti-spermine aptamer (2012)
  • Author(s): 小黒明広
  • Organizer: International Congress on Polyamines
  • Place of Presentation: Istanbul Kultur Univ., Turkey
• [Presentation] スペルミン結合RNAアプタマーのバルジ構造の重要性 (2012)
  • Author(s): 小黒明広
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] RNAアプタマーの解析から明らかにされたスペルミンに結合するRNA構造 (2012)
  • Author(s): 小黒明広
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] Binding manner of anti-spermine aptamer reveals a preferential RNA structure for spermine (2011)
  • Author(s): 小黒 明広
  • Organizer: 第34回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Binding manner of anti-spermine aptamer reveals a preferential RNA structure for spermine (2011)
  • Author(s): 小黒明広
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Isolation and evaluation of anti-polyamine aptamer as a diagnostic tool (2011)
  • Author(s): 小黒明広
  • Organizer: Cold Spring Harbor Lab. Meeting, RNA and Oligonucleotide Therapeutics
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 2011-12-05
• [Presentation] Isolation and evaluation of anti-polyamine aptamer as a diagnostic tool (2011)
  • Author(s): 小黒明広
  • Organizer: Cold Spring Harbor Lab.Meeting, RNA and 0ligonucleotide Therapeutics
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 2011-12-05
• [Presentation] Binding Manner of in vitroSelected RNA against Spermine (2011)
  • Author(s): 小黒明広
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, USA
  • Year and Date: 2011-06-22
• [Presentation] Analyses of the Binding Manner of in vitro Selected RNA to Polyamine (2011)
  • Author(s): 小黒 明広
  • Organizer: RNA 2011 Meeting
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-06-15
• [Presentation] RNA アプタマーを用いたポリアミン検出系の開発 (2011)
  • Author(s): 小黒明広
  • Organizer: 日本ポリアミン学会 第2回年会
  • Place of Presentation: 帝京大学宇都宮キャンパス(栃木県)
  • Year and Date: 2011-01-27
• [Presentation] RNAアプタマーを用いたポリアミン検出系の開発 (2011)
  • Author(s): 小黒明広
  • Organizer: 日本ポリアミン学会第2回年会
  • Place of Presentation: 帝京大学宇都宮キャンパス(栃木県)
  • Year and Date: 2011-01-27
• [Presentation] Binding Manner of in vitro Selected RNA against Spermine (2011)
  • Author(s): 小黒明広
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, USA
• [Presentation] RNA アプタマーを用いたスペルミン検出系の開発 (2010)
  • Author(s): 小黒明広
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] RNAアプタマーを用いたスペルミン検出系の開発 (2010)
  • Author(s): 小黒明広
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Detection of polyamine by RNA aptamer (2010)
  • Author(s): 小黒明広
  • Organizer: 第37回 国際核酸化学シンポジウム
  • Place of Presentation: はまぎんホール ヴィアマーレ(神奈川県)
  • Year and Date: 2010-11-10
• [Presentation] Detection of polyamine by RNA aptamer (2010)
  • Author(s): 小黒明広
  • Organizer: 第37回国際核酸化学シンポジウム
  • Place of Presentation: はまぎんホールヴィアマーレ(神奈川県)
  • Year and Date: 2010-11-10
• [Presentation] Isolation and evaluation of anti-spermine aptamer (2010)
  • Author(s): 小黒明広
  • Organizer: 2010 国際ポリアミン会議
  • Place of Presentation: 御殿場高原リゾート 時之栖 (静岡県)
  • Year and Date: 2010-06-15
• [Presentation] Isolation and evaluation of anti-spermine aptamer (2010)
  • Author(s): 小黒明広
  • Organizer: 2010国際ポリアミン会議
  • Place of Presentation: 御殿場高原リゾート時之栖(静岡県)
  • Year and Date: 2010-06-15