Clinical Application of DNA Methylation Analysis Using Gastric Washes as an ESD Treatment Marker for Early Gastric Cancer

• Project/Area Number: 22501038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: WATANABE Yoshiyuki 聖マリアンナ医科大学, 医学部, 講師 (90329243)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 胃がん / メチル化 / 胃洗浄廃液 / 胃癌 / DNAメチル化 / EMR / ESD / Sox17 / MINT25

Research Abstract

We have developed a new methodology for gastric cancer detection by DNA methylation in gastric washes. It is easy and useful for early detection of recurrence after endoscopic treatment in GCa patients. We now have on going prospective studies.

Research Products

• [Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer. (2012)
  • Author(s): Watanabe Y, Oishi Y, Yoshida Y, Sato Y, Hiraishi T, Oikawa R, Maehata T, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F.
  • Journal Title: Tumour Biol Volume: 33(2) Pages: 383-93
• [Journal Article] Characterization of DNA hypermethylation in two cases of peritoneal mesothelioma. (2012)
  • Author(s): Watanabe Y, Hama R, Shinada K, Yamada Y, Ogata Y, Yoshida Y, TamuraT, Hiraishi T, Oikawa R, Sakurai J, Maehata T, Koizumi H, Itoh F.
  • Journal Title: Tumor Biol. Volume: 33(6) Pages: 2031-40
• [Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer. (2012)
  • Author(s): Watanabe Y et al
  • Journal Title: Tumour Biol Volume: 33(2) Issue: 2 Pages: 383-93
  • DOI: 10.1007/s13277-011-0278-y
  • Peer Reviewed
• [Journal Article] Characterization of DNA hypermethylation in two cases of peritoneal mesothelioma. (2012)
  • Author(s): Yoshiyuki watanabe
  • Journal Title: Tumor Biology Volume: 33(6) Issue: 6 Pages: 2031-2040
  • DOI: 10.1007/s13277-012-0462-8
  • Peer Reviewed
• [Journal Article] Frequent alteration of MLL3 frameshift mutations in microsatellite deficient colorectal cancer. (2011)
  • Author(s): Watanabe Y, Castoro RJ, Kim HS, North B, Oikawa R, Hiraishi T, Ahmed SS, Chung W, Cho MY, Toyota M, Itoh F, Estecio MR, Shen L, Jelinek J, Issa JP.
  • Journal Title: PLos One Volume: 6
• [Journal Article] Gastric wash-based molecular testing for antibiotic resistance in helicobacter pylori. (2011)
  • Author(s): Baba S, Oishi Y, Watanabe Y, Oikawa R, Morita R, Yoshida Y, Hiraishi T, Maehata T, Nagase Y, Fukuda Y, Nakazawa M, Ishigouoka S, Hattori N, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F.
  • Journal Title: Digestion Volume: 84 Pages: 299-305
• [Journal Article] DNA methylation profiles of primary colorectal carcinoma and matched liver metastasis. (2011)
  • Author(s): Watanabe Y, Konishi K, Shen L, Guo Y, Castoro RJ, Kondo K, Chung W, Ahmed S, Jelinek J, Boumber YA, Estecio MR, Maegawa S, Kondo Y, Itoh F, Imawari M, Hamilton SR, Issa JP.
  • Journal Title: PLos One Volume: 6
• [Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer (2011)
  • Author(s): Watanabe Y, et al
  • Journal Title: Tumor Biology Volume: 6
  • Peer Reviewed
• [Journal Article] Gastric wash-based molecular testing for antibiotic resistance in helicobacter pylori (2011)
  • Author(s): Baba S, Watanabe Y, et al
  • Journal Title: Digestion Volume: 84 Pages: 299-305
  • Peer Reviewed
• [Journal Article] DNA methylation profiles of primary colorectal carcinoma and matched liver metastasis (2011)
  • Author(s): Watanabe Y, et al
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] 胃洗浄廃液を用いたMethylated CpG Island Amplification and Microarray (MCAM)法による胃がん分子内視鏡診断 (2010)
  • Author(s): 渡邊嘉行, 他
  • Journal Title: 分子標的薬 その作用機序と効果予測 Volume: 17 Pages: 110-121
• [Journal Article] 胃がんのエピジェネティクス異常と臨床応用 (2010)
  • Author(s): 渡邊嘉行, 他
  • Journal Title: Medical Science Digest Volume: 36(10) Pages: 996-999
• [Journal Article] DNA methylation of interferon regulatory factors in gastric cancer and noncancerous gastric mucosae. (2010)
  • Author(s): Yamashita M, Toyota M, Watanabe Y, Itoh F
  • Journal Title: Cancer Science Volume: 101 Pages: 1708-1716
  • NAID: 10026594987
  • Peer Reviewed
• [Presentation] The BARHL2 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Watanabe Y et al.
  • Organizer: American College of Gastroenterology Annual Meeting 2012.
  • Place of Presentation: Las Vegas NV. Unites States
  • Year and Date: 2012-10-20
• [Presentation] Gastric Wash-Based Sox17 Methylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Watanabe Y et al.
  • Organizer: Digestive Disease Week 2012.
  • Place of Presentation: San Diego CA United States
  • Year and Date: 2012-05-20
• [Presentation] The Sox17 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Hama R, Watanabe Y et al.
  • Organizer: American Association for Cancer Research Annual Meeting 2012.
  • Place of Presentation: Chicago IL. United States
  • Year and Date: 2012-04-02
• [Presentation] The Sox17 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer (2012)
  • Author(s): Ryota Hama
  • Organizer: American Association for Cancer Research Annual Meeting 2012
  • Place of Presentation: Chicago IL United States
• [Presentation] The BARHL2 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer (2012)
  • Author(s): Yoshiyuki Watanabe
  • Organizer: American College of Gastroenterology Annual Meeting 2012
  • Place of Presentation: Las Vegas
• [Presentation] Gastric Wash-Based Sox17 Methylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer (2012)
  • Author(s): Yoshiyuki Watanabe
  • Organizer: Digestive Disease Week 2012
  • Place of Presentation: San Diego CA United States
• [Presentation] Usefulness of DNA methylation analysis using gastric washes as a treatment marker for early gastric cancer (2011)
  • Author(s): Watanabe Y, et al
  • Organizer: 米国癌学会総会AACR 2011
  • Place of Presentation: Orland米国
  • Year and Date: 2011-04-02
• [Presentation] 新たな胃がんESD治療後計画にむけた予測診断マーカーへの応用 (2010)
  • Author(s): 前畑忠輝、渡邊嘉行、伊東文生
  • Organizer: JDDW2010 横浜 日本消化器病週間 シンポジウム16
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2010-10-15
• [Remarks]
  • URL: http://www.marianna-u.ac.jp/chizai/index.html
• [Remarks]
  • URL: http://www.marianna-u.ac.jp/chizai/index.html
• [Patent(Industrial Property Rights)] 胃粘膜洗浄液を利用した疾患関連マーカー検出方法 (2007)
  • Inventor(s): 渡邊 嘉行、豊田 実、今井 浩三、篠村 恭久、伊東 文生、時野 隆至
  • Industrial Property Rights Holder: 学校法人聖マリアンナ医科大学、北海道公立大学法人札幌医科大学
  • Filing Date: 2007-05-15
  • Overseas