| Project/Area Number |
22510022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
HIRAYAMA Hisako 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (90359167)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAI Ken 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクター (80359166)
|
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | メタン酸化菌 / 地球温暖化 / 亜酸化窒素 / ヒドロキシルアミン酸化酵素 / 海洋 |
|---|
| Research Abstract |
The ability of nitrous oxide (N_2O) production by aerobic methanotrophic bacteria was examined by using pure cultures obtained mainly from marine environments. Methylobacter sp. strain, which was isolated in our laboratory from the deep-sea sediment, was revealed to produce significantly more N_2O than other strains tested. The addition of nitrite to the growth medium promoted production of N_2O. The presence of genes encoding hydroxylamine oxidoreductase was examined by DNA extracted from the strains, and the partial nucleotide sequences of the genes were determined.
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