Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Research Abstract |
I have studied functions of the ubiquitin ligases SHPRH and RNF20 by genetically manipulatingthose genes in the chicken DT40 cell line. SHPRH/RAD18 double knockout cells showed enhancedsensitivity towards the DNA-crosslinking agent cisplatin, suggesting a cooperative role of SHPRH andRAD18 in repairing DNA crosslinks. Repression of the RNF20 transgene in RNF20-disrupted cellsresulted in reduced foci formation of the recombination factor RAD51 after ionizing radiation. Thus,RNF20 may facilitate repair of DNA double strand breaks by homologous recombination.
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