Novel functions of DNA repair factors: The roles of new XPD complex in the DNA damage responses

• Project/Area Number: 22510061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka University
• Principal Investigator: SEKI Mineaki 大阪大学, 生命機能研究科, 助教 (40304167)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 修復 / 損傷 / 応答 / DNA修復 / DNA損傷 / DNA損傷応答 / 鉄硫黄クラスター

Research Abstract

To understand the mechanisms of genetics disorders caused by compromised nucleotide excision repair system, we started to analyze the roles of novel complex containing XPD in the response to DNA damage. In the course of study, we found another complex functioning in the cytosolic iron-sulfur (Fe-S) cluster assembly pathway. This complex has central roles in the Fe-S cluster assembly in cytosol. Moreover, we also revealed the roles of each component in the complex.

Research Products

• [Journal Article] IOP1 protein is an external component of the human cytosolic iron-sulfur cluster assembly (CIA) machinery and functions in the MMS19 protein-dependent CIA pathway (2013)
  • Author(s): Mineaki Seki, Yukiko Takeda, Kazuhiro Iwai and Kiyoji Tanaka
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 23 Pages: 16680-16689
  • DOI: 10.1074/jbc.m112.416602
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MMXD, a TFIIH-Independent XPD-MMS19 Protein Complex Involved in Chromosome Segregation. (2010)
  • Author(s): Ito S, et al
  • Journal Title: Molecular Cell Volume: 39 Pages: 632-40
  • Peer Reviewed
• [Journal Article] DNA polymerase theta up-regulation is associated with poor survival in breast cancer, perturbs DNA replication, and promotes genetic instability. (2010)
  • Author(s): Lemee F, et al.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 107 Pages: 13390-5
  • Peer Reviewed
• [Journal Article] Evolutionary conservation of residues in vertebrate DNA polymerase N conferring low fidelity and bypass activity. (2010)
  • Author(s): Takata K, et al.
  • Journal Title: Nucleic Acids Res. Volume: 38 Pages: 3233-44
  • Peer Reviewed
• [Journal Article] Lesion bypass activity of DNA polymerase θ (POLQ) is an intrinsic property of the pol domain and depends on unique sequence inserts. (2010)
  • Author(s): Hogg M, et al.
  • Journal Title: J Mol Biol. Volume: 405 Pages: 642-52
  • Peer Reviewed
• [Presentation] MMS19 is a cytosolic Fe-S assembly factor required for genome integrity. (2012)
  • Author(s): 田中 亀代次
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] MMS19 functions in the cytosolic biogenesis of iron-sulfur proteins required for the maintenance of genomic integrity. (2012)
  • Author(s): 関 峰秋
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] The roles of human MMS19 in cross-link repair and FeS assembly (2011)
  • Author(s): 関峰秋
  • Organizer: 日本環境変異原学会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-22
• [Presentation] The roles of human MMS19 in cross-link repair and FeS assembly. (2011)
  • Author(s): 関 峰秋
  • Organizer: 日本環境変異原学会第40回大会
  • Place of Presentation: 学術総合センター(東京)
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/12/