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Why have nematode a radiation tolerance? :Elucidation of the resistant mechanism.

Research Project

Project/Area Number 22510064
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionTokai University

Principal Investigator

ISHII Naoaki  東海大学, 医学部, 教授 (60096196)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords応答 / ホルミシス効果 / C. elegans / 耐性幼虫 / 放射線傷害 / 寿命 / 線虫 / 耐性機構 / 放射線 / C.elegans / ホルミシス / 遺伝子発現 / マイクロアレイ / 活性酸素 / インスリン・シグナル
Research Abstract

It is known that nematode shows strong radiation tolerance. In response to harsh environmental conditions, nematode C. elegans larvae undergo dauer stage and recover the normal stage by environmental reform. Modest doses X-irradiation of dauer larvae increased their post-dauer longevity. We founded that the gene expressions of some enzymes of metabolic regulation/anti-oxidation related with metabolic regulation/stress response were up-regulated in the X-ray irradiated dauer larvae. Especially, it is suggested that a transcription factor, mxl-3 plays a role of radiation tolerance

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (58 results)

All 2013 2012 2011 2010 Other

All Journal Article (20 results) (of which Peer Reviewed: 17 results) Presentation (30 results) (of which Invited: 2 results) Book (1 results) Remarks (6 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Model animals for the study of oxidative stress from complex II2013

    • Author(s)
      Takamasa Ishii
    • Journal Title

      Biochem. Biophys. Acta.

      Volume: 1827 Issue: 5 Pages: 588-597

    • DOI

      10.1016/j.bbabio.2012.10.016

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress induced inflammation initiates functional decline of tear production2012

    • Author(s)
      Uchino Y, Kawakita T, Miyazawa M, Ishii T, Onouchi H, Yasuda K, Ogawa Y, Shimmura S, Ishii N, Tsubota K.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 10 Pages: e45805-e45805

    • DOI

      10.1371/journal.pone.0045805

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial superoxide anion overproduction in Tet-mev-1 transgenic mice accelerates age-dependent corneal cell dysfunctions2012

    • Author(s)
      Onouchi H, Ishii T, Miyazawa M, Uchino Y, Yasuda K, Hartman PS, Kawai K, Tsubota K, Ishii N
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 53 Issue: 9 Pages: 5780-5787

    • DOI

      10.1167/iovs.12-9573

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of ATP sensor protein in Caenorhabditis elegans2012

    • Author(s)
      J. Kishikawa, M. Fujikawa, H. Imamura, K. Yasuda, H. Noji, N. Ishii, S. Mitani, and K. Yokoyama
    • Journal Title

      Microsc. Res. Tech

      Volume: 75(1) Issue: 1 Pages: 15-9

    • DOI

      10.1002/jemt.21103

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial superoxide anion overproduction in Tet-mev-1 transgenic mice accelerates age-dependent corneal cell dysfunctions.2012

    • Author(s)
      Hiromi Onouchi
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 53 Pages: 5780-5787

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress induced inflammation initiates functional decline of tear production2012

    • Author(s)
      Yuichi Uchino
    • Journal Title

      PLoS ONE

      Volume: 7

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A questionnaire survey on the awareness of recipients of a general health check-up for anti-aging medicine: From knowledge to behavior modification.2012

    • Author(s)
      Yasuhiro Nishizaki
    • Journal Title

      Health Evaluation and Promotion

      Volume: 39 Pages: 519-529

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric arginine dimethylation determines life span in C.elegans by regulating forkhead transcription factor DAF-162011

    • Author(s)
      Takahashi Y., Daitoku H., Hirota K., Tamiya H., Yokoyama A., Kako K., Nagashima Y., Nakamura A., Shimada T., Watanabe S., Yamagata K., Yasuda K., Ishii N., Fukamizu A.
    • Journal Title

      Cell Metabolism

      Volume: 13 Issue: 5 Pages: 505-516

    • DOI

      10.1016/j.cmet.2011.03.017

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A mutation in a mitochondrial dehydrogenase/reductase gene causes an increased sensitivity to oxidative stress and mitochondrial defects in the nematode Caenorhabditis elegans2011

    • Author(s)
      M. Fujii, K. Yasuda, P. S. Hartman, D. Ayusawa, N. Ishii
    • Journal Title

      Genes Cells

      Volume: 16 Issue: 10 Pages: 1022-1034

    • DOI

      10.1111/j.1365-2443.2011.01547.x

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial reactive oxygen species generation by the SDHC V69E mutation causes low birth weight and neonatal growth retardation.2011

    • Author(s)
      Ishii T, Miyazawa M, Onodera A., Yasuda K, Kawabe N, Kirinashizawa M, Yoshimura S. Maruyama N, Hartman PS, Ishii N
    • Journal Title

      Mitochondrion

      Volume: 11 Issue: 1 Pages: 155-165

    • DOI

      10.1016/j.mito.2010.09.006

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interrelationships between mitochondrial fusion, energy metabolism and oxidative stress during development in Caenorhabditis elegans2011

    • Author(s)
      Yasuda K, Hartman PS, Ishii T, Suda H, Akatsuka A, Shoyama T, Miyazawa M, Ishii N
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 404 Pages: 751-755

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondria superoxide anion (O_2^-) inducible "mev-I" animal models for aging research2011

    • Author(s)
      Ishii T, Miyazawa M, Hartman PS, Ishii N
    • Journal Title

      BMB report

      Volume: 44 Issue: 5 Pages: 298-305

    • DOI

      10.5483/bmbrep.2011.44.5.298

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A proposal research for new health check-up items focusing on the future super-aging society in Japan2011

    • Author(s)
      Nishizaki Y, Ishii N, 6番目/6名中
    • Journal Title

      Health Evaluation and Promotion

      Volume: 38 Pages: 537-546

    • NAID

      130004562587

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial reactive oxygen species generation by the SDHC V69E mutation causes low birth weight and neonatal growth retardation2011

    • Author(s)
      Ishii T
    • Journal Title

      Mitochondrion

      Volume: 11 Pages: 155-165

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Radiation Biology of Caenorhabditis elegans: Germ Cell Response, Aging and Behavior2010

    • Author(s)
      Sakashita T, Takanami T, Yanase S, Hamada N, Suzuki M, Kimura T, Kobayashi Y, Ishii N, Higashitani A
    • Journal Title

      Journal of Radiation Research

      Volume: 51 Issue: 2 Pages: 107-121

    • DOI

      10.1269/jrr.09100

    • NAID

      10025914355

    • ISSN
      0449-3060, 1349-9157
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 線虫、C. elegansから始まった寿命決定の分子メカニズム研究2010

    • Author(s)
      石井直明
    • Journal Title

      放射線生物研究

      Volume: 45 Pages: 249-267

    • NAID

      40017338095

    • Related Report
      2012 Final Research Report
  • [Journal Article] Radiation Biology of Caenorhabditis elegans : Germ cell response, aging and behavior2010

    • Author(s)
      Skashita T
    • Journal Title

      Journal of Radiation Research

      Volume: 51 Pages: 107-121

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 老化と活性酸素2010

    • Author(s)
      石井直明
    • Journal Title

      Medical Bio

      Volume: 7 Pages: 18-23

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 線虫、C.elegansから始まった寿命決定の分子メカニズム研究2010

    • Author(s)
      石井直明
    • Journal Title

      放射線生物研究

      Volume: 45 Pages: 249-267

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Interrelationships between mitochondrial fusion, energy metabolism and oxidative stress during development in Caenorhabditis elegans.2010

    • Author(s)
      Yasuda K
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 404 Pages: 751-755

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 寿命決定のメカニズムを探る2013

    • Author(s)
      石井直明
    • Organizer
      日本コエンザイムQ協会 2012年度年会(招待講演)
    • Place of Presentation
      東京工科大学
    • Year and Date
      2013-02-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 寿命決定のメカニズムを探る2013

    • Author(s)
      石井 直明
    • Organizer
      日本コエンザイムQ協会 2012年度年会
    • Place of Presentation
      東京工科大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] The mechanism of longevity with hormesis by the low dose X-irradiation in C. elegans2012

    • Author(s)
      林政太郎、石井直明 他
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] Abnormal lymphocyte accumulation in Tet-mev-1 (SDHC V96E) conditional transgenic mice2012

    • Author(s)
      浅利真司、石井直明 他
    • Organizer
      第35回日本分子生物学会 年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] 線 虫C.elegans における紫外線感受性株rad-8のエネルギー代謝異常2012

    • Author(s)
      安田佳代、石井直明 他
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      東北大学 川内北キャンパス
    • Related Report
      2012 Final Research Report
  • [Presentation] 低線量X線照射による線虫C. elegansの寿命延長機構の解析2012

    • Author(s)
      林政太郎、石井直明 他
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      東北大学 川内北キャンパス
    • Related Report
      2012 Final Research Report
  • [Presentation] 分子生物学的見地からみた放射線(電離放射線)とその健康影響2012

    • Author(s)
      石井直明
    • Organizer
      第21回 日本臨床環境医学会 総会(招待講演)
    • Place of Presentation
      新潟県南魚沼市 市民会館
    • Related Report
      2012 Final Research Report
  • [Presentation] 分子生物学的見地からみた放射線(電離放射線)とその健康影響2012

    • Author(s)
      石井 直明
    • Organizer
      第21回 日本臨床環境医学会 総会
    • Place of Presentation
      新潟県南魚沼市 市民会館
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 低線量X線照射による線虫C. elegansの寿命延長機構の解析2012

    • Author(s)
      林 政太郎
    • Organizer
      日本放射線影響学会 第55回大会
    • Place of Presentation
      東北大学 川内北キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] 線虫C.elegans における紫外線感受性株rad-8のエネルギー代謝異常2012

    • Author(s)
      安田 佳代
    • Organizer
      日本放射線影響学会 第55回大会
    • Place of Presentation
      東北大学 川内北キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] Abnormal lymphocyte accumulation in Tet-mev-1 (SDHC V96E) conditional transgenic mice2012

    • Author(s)
      浅利 真司
    • Organizer
      第35回 日本分子生物学会 年会
    • Place of Presentation
      福岡国際会議譲
    • Related Report
      2012 Annual Research Report
  • [Presentation] The mechanism of longevity with hormesis by the low dose X-irradiation in C. elegans2012

    • Author(s)
      林 政太郎
    • Organizer
      第35回 日本分子生物学会 年会
    • Place of Presentation
      福岡国際会議譲
    • Related Report
      2012 Annual Research Report
  • [Presentation] 線虫54はなぜ放射線に強いか?2011

    • Author(s)
      石井直明
    • Organizer
      放射線影響学会第54回大会
    • Place of Presentation
      神戸商工会議所会館
    • Year and Date
      2011-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 線虫はなぜ放射線に強いか?2011

    • Author(s)
      石井直明
    • Organizer
      放射線影響学会第54回大会
    • Place of Presentation
      神戸商工会議所会館
    • Year and Date
      2011-11-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Oxidative stress-related diseases on aging in mitochondrialO2-inducible mev-1 models.2011

    • Author(s)
      Ishii T, Ishii N et al.
    • Organizer
      The 1st Nagasaki-Pusan Joint Seminar on Aging Research
    • Place of Presentation
      長崎大学(長崎)
    • Year and Date
      2011-02-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Oxidative stress-related diseases on aging in mitochondrial O_<2^-> inducible mev-1 models.2011

    • Author(s)
      Ishii T
    • Organizer
      The 1st Nagasaki-Pusan Joint Seminar on Aging Research
    • Place of Presentation
      長崎大学(長崎)
    • Year and Date
      2011-02-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Energy metabolism abnormality of ultraviolet irradiation sensitive mutant rad-8 in C.elegans.2010

    • Author(s)
      Yasuda K , Ishii N et al.
    • Organizer
      The 7th Conference of ASMRM and 10th Conference of J-mit
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2010-12-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Mitochondrial A-RAF drives neuronal differentiation via superoxide production and PDC activation in mitochondria2010

    • Author(s)
      Miyazawa M, Ishii N et al.
    • Organizer
      The 7th Conference of ASMRM and 10th Conference of J-mit
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2010-12-17
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Effects of mitochondrial oxidative stress by complex II SDHC V69E mutation on decreased fertility, pregnancy and delivery rates2010

    • Author(s)
      Ishii T, Ishii N et al.
    • Organizer
      The 7th Conference ofASMRM and 10th Conference of J-mit
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2010-12-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Effects of mitochondrial oxidative stress by complex II SDHC V69E mutation on decreased fertility, pregnancy and delivery rates.2010

    • Author(s)
      Ishii T
    • Organizer
      The 7th Conference of ASMRM and 10th Conference of J-mit
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Energy metabolism abnormality of ultraviolet irradiation sensitive mutant rad-8 in C.elegans2010

    • Author(s)
      Yasuda K
    • Organizer
      The 7th Conference of ASMRM and 10th Conference of J-mit
    • Place of Presentation
      福岡国際会議譲(福岡)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 加齢性疾患におけるミトコンドリアから生じる酸化ストレスの役割2010

    • Author(s)
      石井直明
    • Organizer
      分子生物学学会・生化学会合同学会
    • Place of Presentation
      国際会議場(神戸)
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] 線虫C.elegans における紫外線感受性株rad-8 のエネルギー代謝異常2010

    • Author(s)
      安田佳代、石井直明 他
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2010-10-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 線虫C.elegansにおける紫外線感受性株rad-8のエネルギー代謝異常2010

    • Author(s)
      安田佳代
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2010-10-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effects of Mitochondrial Superoxide anion (O2-) on Corneal cell functions in Tet-mev-1 transgenic mice2010

    • Author(s)
      Onouchi H, Ishii N et al.
    • Organizer
      XIX Biennial meeting of International Society for Eye Research
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2010-07-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Effects of Mitochondrial Superoxide anion (O_<2^->) on Corneal cell functions in Tet-mev-1 transgenic mice2010

    • Author(s)
      Onouchi H
    • Organizer
      XIX Biennial meeting of International Society for Eye Research
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2010-07-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effects of mitochondrial oxidative stress on corneal cell functions and diseases in Tet-mev-1-mouse2010

    • Author(s)
      Onouchi H, Ishii N et al.
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      名古屋大学(名古屋)
    • Year and Date
      2010-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] ミトコンドリア活性酸素が海馬依存的学習・記憶能力に及ぼす影響2010

    • Author(s)
      柳原倫太郎、石井直明 他
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      名古屋大学(名古屋)
    • Year and Date
      2010-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] ミトコンドリア活性酸素が海馬依存的学習・記憶能力に及ぼす影響2010

    • Author(s)
      柳原倫太郎
    • Organizer
      日本基礎老化学会
    • Place of Presentation
      名古屋大学(名古屋)
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effects of mitochondrial oxidative stress on corneal cell functions and diseases in Tet-mev-1-mouse2010

    • Author(s)
      Onouchi H
    • Organizer
      日本基礎老化学会
    • Place of Presentation
      名古屋大学(名古屋)
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Book] 老年医学 系統講義テキスト2013

    • Author(s)
      石井直明
    • Total Pages
      327
    • Publisher
      西村書店
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://mls.med.u-tokai.ac.jp/jp/index.htm

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.tmhs.jp/

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関係

    • URL

      http://www2.jfn.co.jp/nine/maji/

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関係

    • URL

      http://www.majigofunclub.jp/

    • Related Report
      2012 Final Research Report
  • [Remarks] 東海大学医学部基礎医学系分子生命科学 石井研究室

    • URL

      http://mls.med.u-tokai.ac.jp/jp/index.htm

    • Related Report
      2012 Annual Research Report
  • [Remarks] Tokai Medical Health Science

    • URL

      http://www.tmhs.jp/

    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 外来遺伝子の誘導発現の制御が可能な発現ベクター2011

    • Inventor(s)
      石井直明、石井恭正
    • Industrial Property Rights Holder
      学校法人 東海大学
    • Industrial Property Number
      2006-514867
    • Acquisition Date
      2011-11-09
    • Related Report
      2012 Final Research Report
    • Overseas

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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