| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Research Abstract |
We focused accumulation of polyubiquitinated proteins in cells which reflect protein abnormality to evaluate chemical-induced toxicity with a new index. By analyzing changes in hardly-soluble polyubiquitin chainsdue to exposure to each test substance using six established cell lines, toxic chemicals could be divided into three groups in terms of their toxic effects on levels of cellular abnormal proteins, which were
(1) increased in all cells
(2) increased in a cell type-dependent manner
(3) unchanged in all cells
The present evaluation system is useful for safety management of chemicals based on the understanding of toxic mechanism.
|
