Analysis of Protein adducts derived from environmental carcinogensand their biological activities

• Project/Area Number: 22510076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kanagawa Institute of Technology
• Principal Investigator: TAKAMURA Takeji 神奈川工科大学, 工学部, 教授 (50342910)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 発がん物質 / DNA 付加体 / 環境変異原 / タンパク質付加体 / 発がん性物質 / 3-nitrobenzanthrone

Research Abstract

3-nitrobenzanthrone is a newly discovered environmental carcinogen. In this study,in order to know protein modification with NBA, several experiments were performed. NBA was found to modify lysine, cysteine and tryptophan in moderate yields. Chemical structure of lysine modified with NBA was also revealed by the independentchemical synthesis. This material was found in protein hydrolysates that has beentreated with NBA. Howeveri, in vivo formation of this compound was not assigned inthis study.

Research Products

• [Journal Article] ADP-ribosylation of guanosine bySCO5461 protein secreted fromStreptomyces coelicolor (2013)
  • Author(s): Nakano T, Matsushima-Hibiya Y,Yamamoto M, Takahashi-Nakaguchi A,Fukuda H, Ono M, Takamura-Enya T,Kinashi H, Totsuka Y.
  • Journal Title: Toxicon Volume: 63 Pages: 55-63
  • DOI: 10.1016/j.toxicon.2012.11.019
  • Peer Reviewed
• [Journal Article] Photoassisted defluorination offluorinated substrates andpharmaceuticals by a wide bandgapmetal oxide in aqueous media (2013)
  • Author(s): Hidaka H, Tsukamoto T, Oyama T, Mitsutsuka Y, Takamura T, SerponeN.
  • Journal Title: Photochem Photobiol Sci. Volume: 12 Issue: 5 Pages: 751-759
  • DOI: 10.1039/c2pp25358e
  • Peer Reviewed
• [Journal Article] Evaluation of photo-genotoxicity using the umu test in strains with a high sensitivity to oxidative DNA damage (2011)
  • Author(s): Takamura-Enya T, Ishii R, Oda Y.
  • Journal Title: Mutagenesis Volume: 26, No.4 Issue: 4 Pages: 499-505
  • DOI: 10.1093/mutage/ger008
  • Peer Reviewed
• [Journal Article] BODIPY-modified 2'-deoxyguanosine asa novel tool to detect DNA damages (2011)
  • Author(s): Takamura-Enya, T., Ishii, R.
  • Journal Title: Bioorg. Med. Chem, Lett Volume: 21 Issue: 14 Pages: 4206-4209
  • DOI: 10.1016/j.bmcl.2011.05.084
  • Peer Reviewed
• [Presentation] Evaluation of photo irradiated genotoxic acitivity of phenalenone, an armatic keto compound present in atmospheric environment (2012)
  • Author(s): Takamura-enya, T., Ishi, R., Oda Y.
  • Organizer: Setac Asia Pacific 2012
  • Place of Presentation: ANA Hotel(Kumamoto)
• [Presentation] Evaluation of photogenotoxity ofphenalenone derivatives (2012)
  • Author(s): Ishi R., Takamura-enya T., OdaY.
  • Organizer: Setac AsiaPacific 2012
  • Place of Presentation: ANA Hotel(Kumamoto)
• [Presentation] Carcinogenicity andDNA adduct formation of a mutagenicenvironmental pollutant,3-nitrobenzanthrone (2012)
  • Author(s): Takamura-enya T
  • Organizer: BIT's 5thannual world cancer congress
  • Place of Presentation: Beijing International ConventionCenter, (China)
• [Presentation] 脂肪酸グリシドールの遺伝毒性 (2012)
  • Author(s): 高村岳樹,小田美光
  • Organizer: 日本環境変異原学会第41回大会
  • Place of Presentation: グランシップ静岡(静岡)
• [Presentation] フェナレノン誘導体の遺伝毒性及び光遺伝毒性 (2012)
  • Author(s): 石井亮子,高村岳樹,小田美光,川西優喜,八木孝司
  • Organizer: 日本環境変異原学会第41回大会
  • Place of Presentation: グランシップ静岡(静岡)
• [Presentation] Evaluation of photo irradiated genotoxic acitivity of phenalenone, an armatic keto compound present in atmospheric environment (2012)
  • Author(s): Takamura-enya T. Ishi R. Oda Y.
  • Organizer: Setac Asia Pacific 2012
  • Place of Presentation: ANA Hotel, Kumamoto
• [Presentation] Evaluation of photogenotoxity of phenalenone derivatives (2012)
  • Author(s): Ishi R. Takamura-enya T. Oda Y.
  • Organizer: Setac Asia Pacific 2012
  • Place of Presentation: ANA Hotel, Kumamoto
• [Presentation] Carcinogenicity and DNA adduct formation of a mutagenic environmental pollutant, 3-nitrobenzanthrone (2012)
  • Author(s): Takamura-enya T
  • Organizer: BIT's 5th annual world cancer congress
  • Place of Presentation: Beijing International Convention Center, China
• [Presentation] 脂肪酸グリシドールの遺伝毒性 (2012)
  • Author(s): 高村岳樹，小田美光
  • Organizer: 日本環境変異原学会第４１回大会
  • Place of Presentation: グランシップ静岡，静岡
• [Presentation] フェナレノン誘導体の遺伝毒性及び光遺伝毒性 (2012)
  • Author(s): 石井亮子，高村岳樹，小田美光，川西優喜，八木孝司
  • Organizer: 日本環境変異原学会第４１回大会
  • Place of Presentation: グランシップ静岡，静岡
• [Presentation] 新規DNAアルキル化剤検出試薬の合成と評価 (2011)
  • Author(s): 高村岳樹, 石井亮子
  • Organizer: フォーラム2011衛生薬学・環境トキシコロジー
  • Place of Presentation: 金沢エクセルホテル東急(金沢)
  • Year and Date: 2011-10-26
• [Presentation] DNAアルキル化剤の新規検出試薬の合成と評価 (2011)
  • Author(s): 高村岳樹,石井亮子
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: グランシップ静岡(静岡)
• [Presentation] 大気汚染物質フェナレノンの光遺伝毒性評価 (2011)
  • Author(s): 石井亮子,高村岳樹
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: グランシップ静岡(静岡)
• [Presentation] BODIPY修飾デオキシグアノシンを用いたDNA損傷性物質の検出 (2011)
  • Author(s): 高村岳樹
  • Organizer: 第70会日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
• [Presentation] 新規DNAアルキル化剤検出試薬の合成と評価,フォーラム (2011)
  • Author(s): 高村岳樹,石井亮子
  • Organizer: 2011衛生薬学・環境トキシコロジー
  • Place of Presentation: 金沢エクセルホテル東急(金沢)
• [Presentation] 新規アルキル化剤検出試薬としてのBODIPY-dGの合成,評価 (2011)
  • Author(s): 高村岳樹,石井亮子
  • Organizer: 日本環境変異原学会第40回大会
  • Place of Presentation: 学術総合センター(東京)
• [Presentation] DNA付加体合成の新展開 (2010)
  • Author(s): 高村岳樹
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: エポカル筑波(筑波)