Studies on microbial effects and biotransformation of diphenylarsinic acid in anaerobic soils

• Project/Area Number: 22510083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Niigata University
• Principal Investigator: HARADA Naoki 新潟大学, 自然科学系, 准教授 (50452066)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 環境修復技術 / 土壌圏現象 / 有機ヒ素 / 嫌気的微生物変換 / 硫酸還元 / チオ化 / ジフェニルアルシン酸 / 嫌気分解 / 微生物多様性 / DGGE / 水田土壌 / バイオレメディエーション

Research Abstract

The objectives of this study were to investigate, 1) effects of diphenylarsinic acid (DPAA) on microbial community structures and 2) factors affecting biotransformation of DPAA, in anaerobic soils. As a result, the presence of DPAA did not distinctly change soil bacterial and archaeal community structures under submerged and anaerobic conditions. The microbial transformation of DPAA in soil was enhanced under sulfate-reducing conditions and diphenylthioarsinic acid (DPTA) was found as a major metabolite. Participation of sulfate-reducing bacteria in the transformation of DPAA to DPTA is predicted.

Research Products

• [Journal Article] Effects of diphenylarsinic acid on bacterial and archaeal community structures in an anaerobic paddy soil (2013)
  • Author(s): Guan L, Harada N, Ono Y, Takahashi T, Fujii K, Liu X, Nonaka M
  • Journal Title: Geoderma Volume: 195-196 Pages: 243-250
  • DOI: 10.1016/j.geoderma.2012.12.007
  • Peer Reviewed
• [Journal Article] Enhanced transformation of diphenylarsinic acid in soil under sulfate-reducing conditions (2012)
  • Author(s): Guan L, Hisatomi S, Fujii K, Nonaka M, Harada N
  • Journal Title: Journal of Hazardous Materials Volume: 241-242 Pages: 355-362
  • DOI: 10.1016/j.jhazmat.2012.09.054
  • Peer Reviewed
• [Presentation] A vailability of sulfate and electron donating substrates influences transformation of diphenylarsinic acid in anaerobic soil (2012)
  • Author(s): Guan L, Hisatomi S, Fujii K, Nonaka M, Harada N
  • Organizer: Proceedings of SoilRem 2012SiteRem2012
  • Place of Presentation: Yantai, China
• [Presentation] Biotrans-formation of diphenylarsinic acidunder anaerobic soil conditions (2012)
  • Author(s): Harada N, Guan L, Hisatomi S, NakajimaM, Fujii K, Nonaka M
  • Organizer: Proceedings of SoilRem 2012 SiteRem2012
  • Place of Presentation: Yantai, China
• [Presentation] Determination of a sulfur containing arsenic metabolite in the anaerobic soil culture contaminated with diphenylarsinic acid (2012)
  • Author(s): Hisatomi S, Guan L, Fujii K, Nakajima M, Nonaka M, Harada N
  • Organizer: Proceedings of SoilRem 2012 SiteRem2012
  • Place of Presentation: Yantai, China
• [Presentation] 還元的土壌中におけるジフェニルアルシン酸の変換生成物の同定 (2012)
  • Author(s): 久富志穂子・GUANLing・藤井邦彦・中島真美・野中昌法・原田直樹
  • Organizer: 日本土壌肥料学会鳥取大会講演要旨集
  • Place of Presentation: 鳥取市
• [Presentation] Biotransformation of diphenylarsinic acid under anaerobic soil conditions (2012)
  • Author(s): N Harada, L Guan, S Hisatomi, M Nakajima, K Fujii, M Nonaka
  • Organizer: SoilRem2012 SiteRem2012
  • Place of Presentation: Yantai, China
• [Presentation] Availability of sulfate and electron donating substrates influences transformation of diphenylarsinic acid in anaerobic soil (2012)
  • Author(s): L Guan, S Hisatomi, K Fujii, M Nonaka, N Harada
  • Organizer: SoilRem2012 SiteRem2012
  • Place of Presentation: Yantai, China
• [Presentation] Determination of a sulfur containing arsenic metabolite in the anaerobic soil culture contaminated with diphenylarsinic acid (2012)
  • Author(s): S Hisatomi, L Guan, K Fujii, M Nakajima, M Nonaka, N Harada
  • Organizer: SoilRem2012 SiteRem2012
  • Place of Presentation: Yantai, China
• [Presentation] 還元的土壌中におけるジフェニルアルシン酸の変換生成物の同定 (2012)
  • Author(s): 久富志穂子・Guan Ling・藤井邦彦・中島真美・野中昌法・原田直樹
  • Organizer: 日本土壌肥料学会2012年度鳥取大会
  • Place of Presentation: 鳥取市
• [Presentation] Degradation of diphenylarsinic acid in anaerobic paddy soil and its effects on soil microbial communities (2011)
  • Author(s): Guan L, Ono Y, Takahashi T, Harada N, Fujii K, Liu X, Nonaka M
  • Organizer: International Symposium on Environmental Biogeochemistry XX
  • Place of Presentation: イスタンブール(トルコ共和国)
  • Year and Date: 2011-09-27
• [Presentation] Analysis of soil microbes engaging in anaerobic transformation of diphenylarsinic acid (2011)
  • Author(s): Ling GUAN, Naoki HARADA, Masanori NONAKA
  • Organizer: 第16回ヒ素シンポジウム
  • Place of Presentation: サンアザレア(北海道
  • Year and Date: 2011-02-05
• [Presentation] Impacts of diphenylarsinic acid on microbial diversities in paddy soilamended with rice straw (2011)
  • Author(s): Lillg GUAN, Naoki HARADA, Masanori NONAKA
  • Organizer: The 4th international meeting for the development of integrated pest management in Asia and Africa
  • Place of Presentation: バングラデシュ農業大学(バングラデシュ国)
  • Year and Date: 2011-01-20
• [Presentation] Impacts of diphenylarsinic acid on microbial diversities in paddy soil amended with rice straw (2011)
  • Author(s): Guan L, Harada N, Nonaka M
  • Organizer: The 4th international meeting for the development of integrated pest management in Asia andAfrica
  • Place of Presentation: Mymensingh, Bangladesh
• [Presentation] Analysis of soil microbes engaging in anaerobic transformation of diphenylarsinic acid (2011)
  • Author(s): Guan L, Harada N, NONAKA M
  • Organizer: 第16回ヒ素シンポジウム
  • Place of Presentation: 旭川市
• [Presentation] Degradation of diphenylarsinic acid in anaerobic paddy soil and its effects on soil microbial communities (2011)
  • Author(s): Guan L, Ono Y, Takahashi T, Harada N, Fujii K, Liu X, Nonaka M
  • Organizer: International Symposium on Environmental Biogeochemistry XX
  • Place of Presentation: Istanbul, Turkey