Size-control and functionalization of carbon nanohorns For bioapplication

• Project/Area Number: 22510119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: ZHANG Minfang 独立行政法人産業技術総合研究所, ナノチューブ応用研究センター, 主任研究員 (60518330)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ナノチューブ / ナノホーン / ドラックデリバリーシステム / ナノバイオ / 微小ナノホーン集合体 /  薬物送達システム（DDS） / ステルス性 / サイズ効果 / 薬物送達システム(DDS) / マイクロファジ細胞

Research Abstract

In this study, small-sized aggregates (20-50 nm) or individual carbon nanohorns (S-CNHs) have been successfully separated from 100 nm pristine CNH aggregates (L-CNHs) by oxidative exfoliation with a high yield. The obtained S-CNHs were functionalized by u

Research Products

• [Journal Article] Effect of Nanocarbon size on the cellular uptake (2013)
  • Author(s): M. Zhang, M. Yudasaka
  • Journal Title: Yakugaku Zasshi Volume: 133 Pages: 151-156
  • NAID: 130003361896
• [Journal Article] Size-dependent biodistribution of carbon nanohorns in vivo (2013)
  • Author(s): M. Zhang, T. Yamaguchi, S. Iijima, M. Yudasaka,
  • Journal Title: Nanomedicine Volume: unknown
  • Peer Reviewed
• [Journal Article] A high poly(ethylene glycol) density on graphene nanomaterials reduces the detachment of lipid-poly(ethylene glycol) and macrophage uptake (2012)
  • Author(s): M. Yang, M. Wada, M. Zhang, K. Kostarelos, R. Yuge, S. Iijima, M. Masuda, M. Yudasaka
  • Journal Title: Biomaterialia Volume: 9 Pages: 4744-4753
• [Journal Article] Small-sized carbon nanohorns enabling cellualar uptake control (2012)
  • Author(s): M. Zhang, X. Zhu, T.; S. Iijima, M. Yudasaka
  • Journal Title: Small Volume: 8 Pages: 2524-2531
• [Journal Article] Lysosomal membrane destabilization induced by high accumulation of singe-walled carbon nanohrons in murine macrophage RAW 264.7 (2012)
  • Author(s): Y. Tahara, M. Nakamura, M. Yang, M. Zhang, S. Iijima S. M. Yudasaka
  • Journal Title: Biomaterials Volume: 33 Pages: 2762-2769
• [Journal Article] Small-Sized Carbon Nanohorns Enabling Cellular Uptake Control (2012)
  • Author(s): M. Zhang, X. Zhu, S. Iijima, M. Yudasaka
  • Journal Title: Small Volume: 8 Pages: 4744-4753
  • Peer Reviewed
• [Journal Article] A high poly(ethylene glycol) density on graphene nanomaterials reduces the detachment of lipid–poly(ethylene glycol) and macrophage uptake (2012)
  • Author(s): M. Yang, M. Wada, M. Zhang, K. Kostarelos, R. Yuge, S. Iijima, M. Masuda, M. Yudasaka
  • Journal Title: Acta Biomaterialia Volume: 9 Pages: 4744-4753
  • Peer Reviewed
• [Journal Article] Histological assessments for toxicity and functionalization-dependent biodistribution of carbon nanohorns (2011)
  • Author(s): Y. Tahara, J. Miyawaki, M. Zhang, M. Yang, I. Waga, S. Iijima, H. Irie, M. Yudasaka
  • Journal Title: Nanotechnology Volume: 22 Pages: 265106-265113
• [Journal Article] Lysosomal membrane destabilization induced by high accumulation of (2011)
  • Author(s): 田原善夫、中村真紀、張民芳, et al
  • Journal Title: BIOMATERIALS Volume: 33 Issue: 9 Pages: 2762-2769
  • DOI: 10.1016/j.biomaterials.2011.12.023
  • Peer Reviewed
• [Journal Article] Size-dependent biodistribution of carbon nanohorns in vivo
  • Author(s): M. Zhang, T. Yamaguchi, S. Iijima, M. Yudasaka
  • Journal Title: Nanomedicine Volume: (in press)
  • URL: http://dx.doi.org/10.1016/j.nano.2012.11.011
• [Presentation] Biodegradation of single-wall carbon nanohorns (2013)
  • Author(s): 張民芳、楊梅、Cyrill Bussy,Kostas Kostarelos,飯島澄男、湯田坂雅子
  • Organizer: 第44回フラレン・ナノチューブ学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2013-03-11
• [Presentation] Carbon nanohorns induced RAW264.7 apoptosis: Lysosomal membrane destabilization caused reactive oxygen species generation (2013)
  • Author(s): 楊梅、張民芳、田原善夫、飯島澄男、湯田坂雅子
  • Organizer: 第44回フラレン・ナノチューブ学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2013-03-11
• [Presentation] Biodegradation of Single-Wall Carbon Nanohorns (2013)
  • Author(s): M. Zhang, M. Yang, C. Bussy, H. Ali-Boucetta, S. Iijima, K. Kostarelos, M. Yudasaka
  • Organizer: 第４４回フラーレン・ナノチューブ・グラフェン総合シンポジウム
  • Place of Presentation: 東京
• [Presentation] Carbon nanohorns induced RAW264.7 apoptosis: Lysosomal membrane destabilization caused reactive oxygen species generation, leading cell apoptosis (2013)
  • Author(s): M. Yang, M. Zhang, Y. Tahara, S. Iijima, M. Yudasaka
  • Organizer: 第４４回フラーレン・ナノチューブ・グラフェン総合シンポジウム
  • Place of Presentation: 東京
• [Presentation] uantification of biodistribution of PEG-functionalized single wall carbonnanohorns in mice by using Gd labels (2012)
  • Author(s): 張 民芳、田原 善夫、楊梅、周シン、山口 貴司、飯島 澄男、湯田坂 雅子
  • Organizer: NT13 International Conference on the Science and Application of Nanotubes
  • Place of Presentation: ブリスベン、オストラリア
  • Year and Date: 2012-06-27
• [Presentation] The Fate of PEG-Functionalized Single Wall Carbon Nanohorns after Intravenous injection into mice (2012)
  • Author(s): 張 民芳、田原 善夫、Yang Mei、Zhou Xin、山口 貴司、 飯島 澄男、湯田坂 雅子
  • Organizer: 第42回フラレン・ナノチューブ学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2012-03-08
• [Presentation] The Fate of PEG-Functionalized Single Wall Carbon Nanohorns after Intravenous Injection into Mice (2012)
  • Author(s): 張民芳, et al
  • Organizer: 第42回フラーレン・ナノチューブ・グラフェン学会
  • Place of Presentation: 東京大学
  • Year and Date: 2012-03-08
• [Presentation] High Uptake Cytotoxicity of Single-Walled Carbon Nanohorns in Murine Macrophage RAW264.7 (2012)
  • Author(s): M. Yudasaka, M. Zhang, Y. Tahara, M. Yang, M. Nakamura, S. Iijima
  • Organizer: The 2nd Spain-Japan Symposium on "Carbon Nanoforms"
  • Place of Presentation: Tukuba, Japan
  • Invited
• [Presentation] Interaction of carbon nanohorns with biological systems (2012)
  • Author(s): M. Yudasaka, M. Zhang, M. Yang, M. Nakamura, S. Iijima
  • Organizer: 2012A3 Symposium of Emerging Materials: Nanomaterials for Energy and Environments
  • Place of Presentation: Sendai, Japan
  • Invited
• [Presentation] Quantification of Biodistribution of PEG-Functionalized Single Wall Carbon Nanohorns in Mice by Using Gd2O3 Labels (2012)
  • Author(s): M. Zhang, Y. Tahara, M. Yang, X. Zhou, S. Iijima, M. Yudasaka
  • Organizer: Thirteenth International Conference on the Science and Application of Nanotubes
  • Place of Presentation: Brisbane, Australia
• [Presentation] The Fate of PEG-Functionalized Single Wall Carbon Nanohorns after Intravenous Injection into Mice (2012)
  • Author(s): M. Zhang, Y. Tahara, M. Yang, X. Zhou, S. Iijima, M. Yudasaka
  • Organizer: The 2nd Spain-Japan Symposium on "Carbon Nanoforms"
  • Place of Presentation: Tukuba, Japan
• [Presentation] Searching better lipid-PEG for nano-carbons dispersion in biological application (2011)
  • Author(s): 楊梅 ,和田百代、張民芳、 Kostas Kostarelos, 飯島澄男、増田光俊、湯田坂雅子
  • Organizer: 第41回フラレン・ナノチューブ学会
  • Place of Presentation: 東京(首都大学)
  • Year and Date: 2011-09-05
• [Presentation] Small-sized aggregates of carbon nanohorns enabling cellular uptake control (2011)
  • Author(s): 張民芳、 周シン、 飯島 澄男、湯田坂雅子
  • Organizer: NT12 International Conference on the Science and Application ofNanotubes
  • Place of Presentation: Univ. of Cambridge, UK
  • Year and Date: 2011-07-10
• [Presentation] Small-Sized Aggregates of Carbon Nanohorns Enabling Cellular Uptake Control (2011)
  • Author(s): 張民芳, et al
  • Organizer: NT11 International Conference
  • Place of Presentation: University of Cambridge, UK
  • Year and Date: 2011-07-10
• [Presentation] Size-dependent cellular uptake of carbon nanoparticles (2011)
  • Author(s): 張民芳、 周シン、 飯島 澄男、湯田坂雅子
  • Organizer: 第40回フラレン・ナノチューブ学会
  • Place of Presentation: 名古屋(名城大学)
  • Year and Date: 2011-03-10
• [Presentation] Interaction between carbon nanohorns and amino acids (2011)
  • Author(s): 周 Xin, 張民芳、飯島澄男、湯田坂雅子
  • Organizer: 第40回フラレン・ナノチューブ学会
  • Place of Presentation: 名古屋(名城大学)
  • Year and Date: 2011-03-10
• [Presentation] Evaluation of dispersant effectiveness of lipid-PEG for nano-carbon using carbon nanohorns (2011)
  • Author(s): 楊梅,和田百代、張民芳、Kostas Kostarelos, 飯島澄男、増田光俊、湯田坂雅子
  • Organizer: 第40回フラレン・ナノチューブ学会
  • Place of Presentation: 名古屋(名城大学)
  • Year and Date: 2011-03-08
• [Presentation] ナノカーボンの細胞内取り込みにおけるサイズ依存性 (2011)
  • Author(s): 張民芳
  • Organizer: 第40回フラレン・ナノチューブ学会
  • Place of Presentation: 名古屋・名城大学
  • Year and Date: 2011-03-08
• [Presentation] Toxicology study of single wall carbon nanohorns with different sizes intravenously administrated into mice (2010)
  • Author(s): 張民芳、山口貴司、 飯島澄男、湯田坂雅子
  • Organizer: NT11 International Conference on the Science and Application of Nanotubes
  • Place of Presentation: モントリオール、 カナダ
  • Year and Date: 2010-06-29
• [Book] カーボンナノチューブ・グラフェンハンドブック、"ナノホーン" (2011)
  • Author(s): 中村真紀、張民芳、湯田坂雅子
  • Publisher: コロナ社
• [Book] カーボンナノチューブ・グラフェンハンドブック、3.3.1 (2011)
  • Author(s): 中村真紀、張民芳、湯田坂雅子
  • Total Pages: 3
  • Publisher: コロナ社