Analysis of Wound Healing Process at the Single Cellular Level
| Project/Area Number |
22510122
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AFRIN Rehana 東京工業大学, 大学院・・理工学研究科, 東工 大特別研究員 (70447556)
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| Co-Investigator(Renkei-kenkyūsha) |
猪飼 篤 東京工業大学, イノベーション研究推進体, 特任教授 (50011713)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 分子マニピュレーション / 細胞創傷治癒 / 細胞創傷 / 細胞操作 / 創傷治癒 / 原子間力顕微鏡 / 細胞膜穿孔 / 細胞創傷治癒時間 / ストレスファイバー / 細胞骨格 / 脂質膜分解酵素 |
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| Research Abstract |
From the point of wound healing view, it is important to analyze the repair process of artificially inflicted damages to a live cell. We employed a small glass bead glued at the end of a thin tip of atomic force microscope and coated with enzyme with cell membrane hydrolyzing activity. When the glass bead touched the cell surface, it created a small hole on the cell membrane. First we studied the correlation between the time of repair of the hole and the hole diameter. We noticed that a certain time is required before repair process effectively started. We also noticed that bubble like structures were formed on the cell surface (blebs) and studied the growth and retraction process of them. We also studied the repair process of the damages given to intracellular stress fibers.
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Report
(4 results)
Research Products
(41 results)
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[Presentation] Exploring Atomic Force Microscopy for Nano-medical Applications2012
Author(s)
Afrin, R., Watanabe-Nakayama, T., Machida, S., Saito, M., Ikai, A.
Organizer
ACMM22/ICONN2012/APMC10 Combined Conference
Place of Presentation
Perth, WA, Australia
Related Report
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[Presentation] Interaction of Serum Proteins with the Surface of Hemodialysis Tubing2011
Author(s)
Afirin, R., Watanabe-Nakayama, T., Machida, S., Saito, M., Ikai, A.
Organizer
International Colloquium of Scanning Probe Microscopy (ICSPM19)
Place of Presentation
Toyako, Hokkaido, Japan
Related Report
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[Presentation] Aomic Force Microscopy for Nano-medical Applications2011
Author(s)
Afrin, R., Watanabe-Nakayama, T., Machida, S., Saito, M., Ikai, A.
Organizer
2nd International NanoMedicine Conference
Place of Presentation
Sydney, NSW, Australia
Related Report
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[Presentation] Mechanical Interactions of Red Blood Cell Cytoskeleton and Associated Proteins2011
Author(s)
Afrin, R., Watanabe-Nakayama, T., Machida, S., Saito, M., Ikai, A.
Organizer
13th International Scanning Probe Microscopy Conference (ISPM)
Place of Presentation
Munich, Germany
Related Report
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[Presentation] Interaction Between Serum Proteins And Polymer Surface Studied By Atomic Force Microscopy2011
Author(s)
Afrin, R., Watanabe-Nakayama, T., Machida, S., Saito, M., Ikai, A.
Organizer
AFM-BioMed Conference
Place of Presentation
Paris, France
Related Report
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[Presentation] Exploring Atomic Force Microscopy for Single Cell Manipulations2011
Author(s)
Afrin, R., Watanabe-Nakayama, T., Machida, S., Saito, M., Ikai, A.
Organizer
Annual Meeting of the American Society for Cell Biology
Place of Presentation
Denver, CO, USA
Related Report
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