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Study of Microarray Chips to Evaluate Oxidative Stress Induced by Reactive Oxygen Species

Research Project

Project/Area Number 22510125
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionNippon Institute of Technology

Principal Investigator

BAN Masahito  日本工業大学, 工学部, 教授 (70424059)

Research Collaborator SASAKI Fusako  日本工業大学, 工学研究科, 大学院生
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsフラーレン / インクジェット / マイクロアレイチップ / 活性酸素 / 酸化ストレス / 細胞毒 / 細胞 / ナノチューブ・フラーレン / マイクロ・ナノデバイス / マイクロアレイ / ストレス
Research Abstract

The crystalline C_ particleswere three-dimensionally synthesized into m I c r o wells using an ink-jet method, and the PDMS-based microarraychips with reactive oxygenspecies (R O S) generation sources, which generate ROS under visible light irradiation, were fabricated. The cell culture tests using the microarray chips revealed that cytotoxicity induced by ROS was quantitatively evaluated, resulting in creation of the devices to evaluate cellular response to oxidative stress.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 3 results) Presentation (16 results)

  • [Journal Article] Cytotoxicity of photosensitizing crystalline C_ particles formed by ink-jet method2012

    • Author(s)
      M. Ban, Y. Kogi, F. Sasaki
    • Journal Title

      J. Imaging Society of Japan

      Volume: 51 Pages: 496-500

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation of photosensitizingcrystalline C_ particles by ink-jet method2012

    • Author(s)
      M. Ban, F. Sasaki
    • Journal Title

      World Journal of Nano Science and Engineering

      Volume: 2 Issue: 02 Pages: 110-115

    • DOI

      10.4236/wjnse.2012.22014

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of microstructures formed by plasma and ink-jet processing to cell culture2012

    • Author(s)
      M. Ban
    • Journal Title

      日本工業大学研究報告

      Volume: 42 Pages: 87-88

    • Related Report
      2012 Final Research Report
  • [Journal Article] Cytotoxicity of Photosensitizing Crystalline C60 Particles Formed by Ink-jet Method2012

    • Author(s)
      M. Ban, Y. Kogi, F. Sasaki
    • Journal Title

      Journal of the Imaging Society of Japan

      Volume: 51 Pages: 496-500

    • NAID

      130004484017

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of Microstructures Formed by Plasma and Ink-jet Processing to Cell Culture2012

    • Author(s)
      M. Ban
    • Journal Title

      日本工業大学研究報告

      Volume: 42 Pages: 87-88

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 「活性酸素を生成するC60結晶微粒子のマイクロアレイチップへの応用」に関するμTAS 2010での研究発表2012

    • Author(s)
      伴雅人
    • Journal Title

      日本工業大学研究報告

      Volume: 42 Pages: 175-176

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Application of inkjet-fabricated crystalline C_ particles generating reactive oxygen species under visible light irradiation to microarray chips2011

    • Author(s)
      M. Ban
    • Journal Title

      日本工業大学研究報告

      Volume: 41 Pages: 73-74

    • Related Report
      2012 Final Research Report
  • [Journal Article] 活性酸素生成源をもつマイクロアレイチップ2011

    • Author(s)
      伴雅人
    • Journal Title

      日本工業大学研究報告

      Volume: 41 Pages: 17-20

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Application of Inkjet-fabricated Crystalline C_<60> Particles Generating Reactive Oxygen Species under Visible Light Irradiation to Microarray Chips2011

    • Author(s)
      M.Ban
    • Journal Title

      日本工業大学研究報告

      Volume: 41 Pages: 73-74

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Crystalline C_ particleformationin PDMS-based microchamber by the ink-jet method2010

    • Author(s)
      M. Ban
    • Journal Title

      日本工業大学研究報告

      Volume: 40 Pages: 55-56

    • Related Report
      2012 Final Research Report
  • [Journal Article] Crystalline C_<60> Particle Formation in PDMS-based Microchamber by the Ink-jet Method2010

    • Author(s)
      伴雅人
    • Journal Title

      日本工業大学研究報告

      Volume: 40 Pages: 481-482

    • Related Report
      2010 Annual Research Report
  • [Journal Article] インクジェット法によるC_<60>結晶微粒子の形成とその活性酸素生成評価2010

    • Author(s)
      伴雅人
    • Journal Title

      日本工業大学研究報告

      Volume: 40 Pages: 444-445

    • Related Report
      2010 Annual Research Report
  • [Presentation] Control of cell death by green laser irradiation using PDMS-based microarray chip2012

    • Author(s)
      M. Ban, F. Sasaki, Y. Kogi
    • Organizer
      International Symposium on Microchemistry and Microsystems (ISMM2012)
    • Place of Presentation
      Hsinchu, Taiwan
    • Year and Date
      2012-06-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Control of Cell Death by Green Laser Irradiation using PDMS-based Microarray chip2012

    • Author(s)
      M. Ban, F. Sasaki, Y. Kogi
    • Organizer
      International Symposium on Microchemistry and Microsystems (ISMM) 2012
    • Place of Presentation
      Hsinchu, Taiwan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 可視光照射によるC60結晶微粒子の細胞毒性評価2012

    • Author(s)
      古城裕矢、伴雅人
    • Organizer
      日本バイオマテリアル学会シンポジウム2012
    • Place of Presentation
      仙台国際センター、仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] インクジェット法にて形成したフラーレン結晶微粒子による細胞毒性2011

    • Author(s)
      伴雅人、佐々木扶紗子、古城裕矢
    • Organizer
      第24回ダイヤモンドシンポジウム
    • Place of Presentation
      産総研、つくば
    • Year and Date
      2011-12-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Application of microstructures formed by plasma and ink-jet processing to cell culture2011

    • Author(s)
      M. Ban
    • Organizer
      BIT's 1st Annual World Congress of Nano Science & Technology 2011
    • Place of Presentation
      Dalian, China
    • Year and Date
      2011-10-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of Microstructures Formed by Plasma and Ink-jet Processing to Cell Culture2011

    • Author(s)
      M.Ban
    • Organizer
      BIT's 1^<st> Annual World Congress of Nano Science & Technology 2011
    • Place of Presentation
      Dalian, China
    • Year and Date
      2011-10-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cytotoxicity of crystalline C_ particles encapsulated in PDMS-based microwells by ink-jet method2011

    • Author(s)
      F. Sasaki, M. Ban
    • Organizer
      International Symposium on Microchemistry and Microsystems (ISMM) 2011
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2011-06-02
    • Related Report
      2012 Final Research Report
  • [Presentation] Cytotoxicity of Crystalline C_<60> Particles Encapsulated in PDMS-Based Microwells by Ink-jet Method2011

    • Author(s)
      F.Sasaki, M.Ban
    • Organizer
      International Symposium on Microchemistry and Microsystems (ISMM) 2011
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2011-06-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] インクジェットにより形成されるC_<60>結晶微粒子の寸法・形態に関する考察2011

    • Author(s)
      小林光太郎、佐々木扶紗子、伴雅人
    • Organizer
      第123回表面技術協会講演大会
    • Place of Presentation
      関東学院大学、神奈川
    • Year and Date
      2011-03-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] インクジェットにより形成したフラーレン結晶微粒子の活性酸素生成2010

    • Author(s)
      小林光太郎、佐々木扶紗子、伴雅人
    • Organizer
      第22回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      名古屋大学、名古屋
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Application of inkjet-fabricated crystalline C60 particles generatingreactive oxygen species under visible light irradiation to microarray chips2010

    • Author(s)
      F. Sasaki, M. Ban
    • Organizer
      The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Groningen, Netherlands
    • Year and Date
      2010-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of Inkjet-fabricated Crystalline C_<60> Particles Generating Reactive Oxygen Species under Visible Light Irradiation to Microarray Chips2010

    • Author(s)
      F.Sasaki, M.Ban
    • Organizer
      The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μ TAS 2010)
    • Place of Presentation
      Groningen, Netherlands
    • Year and Date
      2010-10-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Generation of reactive oxygen species by crystalline C_ particles synthesized using the ink-jet method2010

    • Author(s)
      F. Sasaki, M. Ban
    • Organizer
      Carbon 2010 Extended Abstracts, Clemson
    • Place of Presentation
      SC, USA
    • Year and Date
      2010-07-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Generation of Reactive Oxygen Species by Crystalline C_<60> Particles Synthesized Using the Ink-jet Method2010

    • Author(s)
      F.Sasaki, M.Ban
    • Organizer
      Carbon 2010
    • Place of Presentation
      Clemson SC, USA
    • Year and Date
      2010-07-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Photosensitizing crystalline C60particles encapsulated in PDMS-based microchamber by ink-jet method2010

    • Author(s)
      F. Sasaki, T. Hirose, M. Ban
    • Organizer
      International Symposium on Microchemistry and Microsystems (ISMM)
    • Place of Presentation
      Hong Kong, China
    • Year and Date
      2010-05-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Photosensitizing Crystalline C_<60> Particles Encapsulated in PDMS-Based Microchamber by Ink-jet Method2010

    • Author(s)
      F.Sasaki, T.Hirose, M.Ban
    • Organizer
      International Symposium on Microchemistry and Microsystems (ISMM) 2010
    • Place of Presentation
      Hong Kong, China
    • Year and Date
      2010-05-29
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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