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Analysis of regulation mechanism for metabolic pathway based on comparative proteomics for protein abundance

Research Project

Project/Area Number 22510207
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genome biology
Research InstitutionMeiji University

Principal Investigator

KITO Keiji  明治大学, 農学部, 専任講師 (40345632)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords比較プロテオミクス / 代謝経路 / 酵母 / 質量分析 / 出芽酵母 / 絶対量計測 / PCS-MS法
Research Abstract

In this study, we analyzed proteome expression pattern for multiple yeast species grown on different culture conditions to understand proteome differences involved in interspecies varieties. Eight yeast species grew with comparable growth rate on glucose medium. In contrast, four species of Saccharomyces genus, including S. cerevisiae, showed slow growth rate by two-to three-fold on glycerol and acetate medium, compared to four species belonging to different genus. Some groups of proteins involved in mitochondrial metabolic pathway and response to oxidative stress showed different abundance between Saccharomyces and other genus when grown on glycerol. It is suggested that such differences in abundance of proteins with specific functions could be responsible for disparity of growth rate among yeast species.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (7 results)

All 2012 2010

All Journal Article (1 results) Presentation (6 results)

  • [Journal Article] Remodeling of the SCF complex-mediated ubiquitination system by compositiona lalteration of incor-porated F-box proteins.2010

    • Author(s)
      Kato,M.,Kito,K.,Ota,K.,Ito,T.
    • Journal Title

      Proteomics

      Volume: 10 Pages: 115-123

    • Related Report
      2012 Final Research Report
  • [Presentation] 酵母種間における比較プロテオミクス2012

    • Author(s)
      大西美帆子、伊藤遼、長谷川勇輔、正岡昌一郎、紀藤圭治
    • Organizer
      日本プロテオーム学会2012年大会
    • Related Report
      2012 Final Research Report
  • [Presentation] 酵母種間における代謝経路の比較プロテオミクス2012

    • Author(s)
      大西美帆子、伊藤遼、長谷川勇輔、鈴木 一司、野原健弘、 紀藤圭治
    • Organizer
      第35回日本分子生物学会年会
    • Related Report
      2012 Final Research Report
  • [Presentation] 酵母種間における比較プロテオミクス2012

    • Author(s)
      紀藤圭治
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      日本科学未来館
    • Related Report
      2012 Annual Research Report
  • [Presentation] 酵母種間における代謝経路の比較プロテオミクス2012

    • Author(s)
      紀藤圭治
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Hierarchical Use of Peptide-concatenated Standard (PCS) for Absolute Protein Quantification with Mass Spectrometry.2010

    • Author(s)
      Keiji Kito, Takashi Ito
    • Organizer
      Human Proteome World Congress
    • Place of Presentation
      Sydney Convention and Exhibition Centre, NSW, Australia
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hierarchical Use of Peptide-concatenated Standard (PCS) for Absolute Protein Quantification with Mass Spectrometry.2010

    • Author(s)
      Keiji Kito, Takashi Ito.
    • Organizer
      September 2010.In Human Proteome World Congress
    • Place of Presentation
      Sydney
    • Year and Date
      2010-09-21
    • Related Report
      2012 Final Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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