Analysis of regulation mechanism for metabolic pathway based on comparative proteomics for protein abundance
| Project/Area Number |
22510207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Meiji University |
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Principal Investigator |
KITO Keiji 明治大学, 農学部, 専任講師 (40345632)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 比較プロテオミクス / 代謝経路 / 酵母 / 質量分析 / 出芽酵母 / 絶対量計測 / PCS-MS法 |
|---|
| Research Abstract |
In this study, we analyzed proteome expression pattern for multiple yeast species grown on different culture conditions to understand proteome differences involved in interspecies varieties. Eight yeast species grew with comparable growth rate on glucose medium. In contrast, four species of Saccharomyces genus, including S. cerevisiae, showed slow growth rate by two-to three-fold on glycerol and acetate medium, compared to four species belonging to different genus. Some groups of proteins involved in mitochondrial metabolic pathway and response to oxidative stress showed different abundance between Saccharomyces and other genus when grown on glycerol. It is suggested that such differences in abundance of proteins with specific functions could be responsible for disparity of growth rate among yeast species.
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Report
(4 results)
Research Products
(7 results)
