Proteomic analysis of bacterial translation by iso-electric point unlimited RFHR 2D PAGE
| Project/Area Number |
22510210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | 株式会社吉田生物研究所 (2012)
Yoshida Biological Laboratry, Bio-informatics section (2010-2011) |
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Principal Investigator |
WADA Akira 株式会社吉田生物研究所, バイオ情報研究部門, 部門長 (80025387)
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| Co-Investigator(Kenkyū-buntansha) |
UETA Masami 株式会社吉田生物研究所, バイオ情報研究部, 研究員 (30512511)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 翻訳 / RFHR法 / プロテオミクス / リボソーム / 100Sリボソーム / 源核生物 / 原核生物 |
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| Research Abstract |
Quantitative and separating ability of RFHR 2D PAGE was improved, and it was applied to proteomics of bacterial translation. In 15 bacterial strains proteins forming 100S ribosome(70S dimer) were determined. It is RMF homolog in proteobacteria γgroup, and long HPF homolog in other bacteria. Therefore two distinct 100S ribosomes exist in bacteria.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Conservation of two distinct types of 100S ribosome in bacteria2013
Author(s)
Masami Ueta, Chieko Wada, Takashi Daifuku, Yoshihiko Sako, Yoshitaka Bessho, Aya Kitamura, Ryosuke L. Ohniwa, Kazuya Morikawa, Hideji Yoshida, Takayuki Kato, Tomoko Miyata, Keiichi Namba and Akira Wada
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Journal Title
Genes to Cells
Volume: 18(in press)
Related Report
Peer Reviewed
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[Journal Article] Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis2010
Author(s)
Kousei Kimura , Akira Wada Masami Ueta , Ogata A, Tanaka S, Sakai A, Yoshida H, Fushitani H, Miyamoto A, Fukushima M, Uchiumi T, Tanigawa N.
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Journal Title
Int J Oncol.
Volume: 37
Pages: 1271-1278
Related Report
Peer Reviewed
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