| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Quantitative and separating ability of RFHR 2D PAGE was improved, and it was applied to proteomics of bacterial translation. In 15 bacterial strains proteins forming 100S ribosome(70S dimer) were determined. It is RMF homolog in proteobacteria γgroup, and long HPF homolog in other bacteria. Therefore two distinct 100S ribosomes exist in bacteria.
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