Identification and functional analysis of the allele-specific DNA-binding factors that recognize type 2 diabetes susceptibility SNPs using nanobeads technology

• Project/Area Number: 22510216
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: HIRAMOTO Masaki 独立行政法人国立国際医療研究センター, 代謝疾患研究部, 室長 (70297828)
• Co-Investigator(Renkei-kenkyūsha): YASUDA Kazuki 独立行政法人国立国際医療研究センター, 代謝疾患研究部, 部長 (80311611) ; UDAGAWA Haruhide 独立行政法人国立国際医療研究センター, 代謝疾患研究部, 研究員 (50533882)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 2 型糖尿病 / 一塩基多型 / ナノビーズ / ゲノム / 糖尿病 / 疾患感受性遺伝子

Research Abstract

Single nucleotide polymorphisms (SNPs) in KCNQ1 gene locus confer susceptibility to type 2 diabetes. In this study, we identified the proteins that bind this locus in allele-specific manner using nanobeads technology, and showed that the identified proteins can participate in the regulation of gene expressions. This means that SNPs in KCNQ1 gene modulate the affinity of the locus for DNA-binding proteins, which possibly change gene expressions and consequently increase risk of type 2 diabetes.

Research Products

• [Presentation] Type 2 Diabetes-Associated SNPs within KCNQ1 Gene Modulate the Affinity of the Locus for DNA-Binding Factors. (2013)
  • Author(s): Hiramoto M, Udagawa H, Watanabe A, Kawaguchi M, Nishimura W, Nammo T, Yasuda K
  • Organizer: 73^<rd> Scientific Sessions, American Diabetes Association
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2013-06-24
• [Presentation] KCNQ1遺伝子イントロンのSNP領域においてアリル特異的に結合する因子の単離・同定・解析 (2012)
  • Author(s): 平本正樹, 宇田川陽秀, 渡邉淳, 上番 増喬, 川口美穂, 石橋奈緒子, 南茂隆 生, 西村渉, 安田和基
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2012-05-18
• [Presentation] KCNQ1遺伝子イントロンのSNP領域においてアリル特異的に結合する因子の単離・同定・解析 (2012)
  • Author(s): 平本正樹
  • Organizer: 日本糖尿病学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2012-05-18
• [Presentation] KCNQ1 遺伝子イントロンにおける2 型糖尿病感受性SNP の機能解析 (2011)
  • Author(s): 平本正樹, 宇田川陽秀, 渡邉淳, 上番 増喬, 川口美穂, 南茂隆生, 西村渉, 安田和基
  • Organizer: 日本人類遺伝学会第56回大会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-11
• [Presentation] KCNQ1遺伝子イントロンにおける2型糖尿病感受性SNPの機能解析 (2011)
  • Author(s): 平本正樹
  • Organizer: 日本人類遺伝学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-11