| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
In this study, I developed a plant-optimized version of cNLS Mapper, a prediction tool of nuclear localization signals, by creating an activity-based profile of a plant-specific NLS class. I also developed a computational tool (NESmapper) for nuclear export signal (NES) prediction, by using newly created activity-based profiles for all the NES subclasses An evaluation test for NES prediction with test data sets showed that NESmapper predicted over 80% of positive NESs with ≤10% of false positive rate. Comparison of the prediction performance of NESmapper with those of other available tools (i.e., NESsential, NetMES, and Wregex) demonstrated that NESmapper outperforms other tools. NESmapper and also its combined version with cNLS Mapper will be provided for the scientific community as a valuable tool to predict functional NESs and NLSs.
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