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Utilization of biomass resources based on genome analyses of acetic acid bacteria

Research Project

Project/Area Number 22510222
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied genomics
Research InstitutionKinki University

Principal Investigator

AZUMA Yoshinao  近畿大学, 生物理工学部, 准教授 (90333509)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords酢酸菌 / Acetobacter pasteurianus / バイオエタノール / グリセロール代謝 / 代謝マップ / Gluconacetobacter xylinus / ゲノム解析 / バクテリア・セルロース / G. xylinus
Research Abstract

Acetic acid bacteria (AAB) are characterized by their ability to transform alcohols and sugar-alcohols into their corresponding organic acids. To acquire new metabolic systems practicable in high sugar concentration and to achieve a model system to comprehend environmental responses of AAB, we have performed comparative genome, transcriptome and proteome analyses First, to determine draft genome DNA sequences of 25 AAB, we carried out DNA sequencing, assembling and gene extraction by using a next generation sequencer. We will present our recent data for the metabolic and gene regulations by the stress of high glucose concentration.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (31 results) (of which Invited: 1 results) Book (3 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Preparation of carboxylated Ag nanoparticles as a coating material for medical devicesand control of antibacterial activity2013

    • Author(s)
      T. Furuzono, T. Iwamoto, Y. Azuma, M. Okada, Y. Sawa
    • Journal Title

      Journal of Artificial Organs

      Volume: in printing

    • Related Report
      2012 Final Research Report
  • [Journal Article] Complete genome sequence of a cellulose-producing bacterium, Gluconacetobacter xylinus NBRC 3288, isolated from vinegar.2011

    • Author(s)
      H. Ogino, Y. Azuma, H. Nakazawa, M. Matsutani, A. Hasegawa, K. Otsuyama, K. Matsushita, N. Fujita, M. Shirai
    • Journal Title

      J. Bacteriology

      Volume: 193 (24) Pages: 6997-8

    • Related Report
      2012 Final Research Report
  • [Journal Article] Complete genome sequence of a cellulose-producing bacterium, Gluconacetobacter xylinus NBRC 3288, isolated from vinega2011

    • Author(s)
      H.Ogino, Y.Azuma, H.Nakazawa, M.Matsutani, A.Hasegawa, K.Otsuyama, K.Matsushita, N.Fujita, M.Shirai
    • Journal Title

      J.Bacteriology

      Volume: 193 Pages: 6997-6998

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tanticharoenia sakaeratensisの高濃度グルコース耐性・代謝に関するプロテオーム解析2011

    • Author(s)
      波多野裕美、武部聡、東慶直
    • Journal Title

      近畿大学生物理工学部紀要

      Volume: 28 Pages: 95-108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic instability of Aeetobacter pasteurianus revealed by wholee genome analyses2011

    • Author(s)
      東慶直
    • Journal Title

      近畿大学生物理工学部紀要

      Volume: 27

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Genome analyses for hyper glucose tolerance of acetic acid bacteria2013

    • Author(s)
      H. Hadano, N. Higashiura, H. Hirakawa, S. Takebe, K. Matsushita, Y. AZUMA
    • Organizer
      Tanticharoenia sakaeratensis and Asaia bogorensis.、2013年度日本農芸化学会大会
    • Place of Presentation
      東北大学
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Genome Analyses for High Glucose Tolerance of Acetic Acid Bacteria2013

    • Author(s)
      H. Hadano, N. Higashiura, H. Hirakawa, S. Takebe, K. Matsushita, Y. Azuma
    • Organizer
      113rd General Meeting: American society for Microbiology
    • Place of Presentation
      Denver, USA
    • Year and Date
      2013-03-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 酢酸菌のストレス応答に関するゲノム解析~耐糖性の例~2013

    • Author(s)
      波多野裕美、東裏典枝、高見晶子、平川英樹、武部 聡、松下一信、東 慶直
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      2013-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 病原性酢酸菌Asaia bogorensis のゲノム解析2013

    • Author(s)
      東裏典枝、波多野裕美、高見晶子、平川英樹、武部 聡、松下一信、東 慶直
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      2013-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 酢酸菌のストレス応答に関するゲノム解析2013

    • Author(s)
      東 慶直
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大
    • Year and Date
      2013-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Genome analysis for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis2012

    • Author(s)
      H. Hadano, K. Omura, H. Hirakawa, S. Takebe, K. Matsushita, Y. Azuma.
    • Organizer
      第3回国際酢酸菌学会
    • Place of Presentation
      Cordoba Univ., Spain
    • Year and Date
      2012-04-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Genome analysis for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis.2012

    • Author(s)
      H. Hadano, K. Omura, H. Hirakawa, S. Takebe, K.. Matsushita, Y. Azuma
    • Organizer
      第3回国際酢酸菌学会
    • Place of Presentation
      Cordoba Univ., Spain
    • Year and Date
      2012-04-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 酢酸菌Tanticharoenia sakaeratensisの有する高濃度グルコース耐性に対するゲノム解析2012

    • Author(s)
      波多野裕美、平川英樹、松下一信、武部聡、東慶直
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] degenerate PCRを用いたBacillus thuringiensis由来の殺虫タンパク質遺伝子の検出と単離2012

    • Author(s)
      森村夏花、波多野裕美、久保友梨佳、小宮浩史、唐谷ゆふ、東慶直、武部聡
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微生物のゲノム解析ー病原性菌から有用細菌まで2012

    • Author(s)
      東 慶直
    • Organizer
      第9回アグリバイオ・セミナー
    • Place of Presentation
      奈良市
    • Year and Date
      2012-03-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 微生物のゲノム解析-病原性菌から有用細菌まで-2012

    • Author(s)
      東慶直
    • Organizer
      アグリバイオ・セミナー
    • Place of Presentation
      近畿大学農学部(奈良県)
    • Year and Date
      2012-03-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genome analysis for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis.2012

    • Author(s)
      Yoshinao Azuma
    • Organizer
      第3回国際酢酸菌学会
    • Place of Presentation
      Cordoba Univ., Spain
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高濃度グルコース耐性酢酸菌Tanticharoenia sakaeratensisのゲノム・プロテオーム解析2011

    • Author(s)
      波多野裕美, 武部聡, 東慶直
    • Organizer
      分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 産業有用微生物のストレス耐性化育種とそのゲノム情報解析2011

    • Author(s)
      東 慶直
    • Organizer
      わかやまテクノゼジネスフェア
    • Place of Presentation
      和歌山市
    • Year and Date
      2011-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 産業有用微生物のストレス耐性化育種とそのゲノム情報解析2011

    • Author(s)
      東慶直
    • Organizer
      わかやまテクノビジネスフェア
    • Place of Presentation
      ホテルアバローム紀の国(和歌山県)
    • Year and Date
      2011-12-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Degenerate PCR based search for genes encoding insecticidal proteins of Bacillus thuringiensis2011

    • Author(s)
      Yuta Sugimori, Yu Karatani, Hiromi Hadano, Yoshinao Azuma, So Takebe
    • Organizer
      国際微生物学会
    • Place of Presentation
      国際会議場(札幌)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phenome analyses of the thermo-tolerant strain bred from Acetobacter pasteurianus2011

    • Author(s)
      Yoshinao Azuma, Naoko Furuya
    • Organizer
      国際微生物学会
    • Place of Presentation
      国際会議場(札幌市)
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genome and proteome analyses for hyper glucose tolerance of an acetic acid bacterium, Tanticharoenia sakaeratensis2011

    • Author(s)
      Hiromi Hadano, Yuta Sugimori, So Takebe, Yoshinao Azuma
    • Organizer
      国際微生物学会
    • Place of Presentation
      国際会議場(札幌市)
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] ANALYSIS OF THERMAL ADAPTATION MECHANISM OF ACETOBACTER PASTEURIANUS SKU1108 USING NEXT GENERATION GENOME SEQUENCER2011

    • Author(s)
      Minenosuke Matsutani, Natsaran Saichana, Mitsuteru Nishikura, Toshiharu Yakushi, Yoshinao Azuma, Kazunobu Matsushita
    • Organizer
      国際微生物学会
    • Place of Presentation
      国際会議場(札幌市)
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] IDENTIFICATION OF GENES THAT CONTRIBUTE TO THE TEMPERATURE-RESISTANCE IN ACETIC ACID BACTERIUM BY WHOLE-GENOME ANALYSIS2011

    • Author(s)
      Hidetaka Ogino, Yoshinao Azuma, Minenosuke Matsutani, Mutsunori Shirai
    • Organizer
      国際微生物学会
    • Place of Presentation
      国際会議場(札幌市)
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高濃度グルコース耐性菌Tanticharoenia sakaeratensisのプロテオーム解析2011

    • Author(s)
      波多野裕美、武部聡、東慶直
    • Organizer
      日本農芸化学会関西支部会
    • Place of Presentation
      京都府立大学(京都府)
    • Year and Date
      2011-05-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Tanticharoenia sakaeratensisの高濃度グルコース耐性の解析2011

    • Author(s)
      波多野裕美
    • Organizer
      2011年度日本農芸化学会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Acetobacter pasteurianus高温耐性育種株のphenome解析2011

    • Author(s)
      森村夏花
    • Organizer
      2011年度日本農芸化学会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genome-wide analysis of thermal adaptation mechanism of Acetobacater pasteurianus SKU1108 using mext generation genome sequencer2010

    • Author(s)
      松谷峰之介
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ボートアイランド(兵庫)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bacillus thuringiensisがもつ新規殺虫タンパク質遺伝子の探索及び構造解析2010

    • Author(s)
      唐谷ゆふ
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ボートアイランド(兵庫)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ドラフトゲノム解析による耐熱性酢酸菌Acettobacter pasteurianus SKU1108株の耐熱化磯構の解析2010

    • Author(s)
      松谷峰之介
    • Organizer
      第2回酢酸菌研究会
    • Place of Presentation
      玉川大学(東京)
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] タンパク質安定化因子4AaCterタグを導入したBt用発現ベクターの構築2010

    • Author(s)
      佐藤一樹
    • Organizer
      第9回昆虫病理研究会シンポジウム第16回BT研究会合同大会
    • Place of Presentation
      山梨県財団法人人材開発センター富士研修所
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 殺虫性を示すBt3菌株がもつcry遺伝子のdegenerate PCRによる探索2010

    • Author(s)
      唐谷ゆふ
    • Organizer
      第9回昆虫病理研究会シンポジウム第16回BT研究会合同大会
    • Place of Presentation
      山梨県財団法人人材開発センター富士研修所
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Degenerate PCR based search for genes encoding insecticidal proteins of Bacillus thuringiensis2010

    • Author(s)
      Yu Karatani
    • Organizer
      43rd Annual meeting of the society for invertebrate pathology
    • Place of Presentation
      Karadeniz Technical University, Trabzon Turkey
    • Year and Date
      2010-07-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genome analyses for hyper glucose tolerance of an acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis

    • Author(s)
      Yoshinao Azuma
    • Organizer
      第4回酢酸菌研究会
    • Place of Presentation
      山口大学(山口県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 酢酸菌の耐熱化,あれやこれや

    • Author(s)
      東 慶直
    • Organizer
      山口大学 第4回 発酵微生物部門セミナー
    • Place of Presentation
      山口大学(山口県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] 「酢の機能と科学」酢酸菌研究会編2012

    • Author(s)
      東 慶直
    • Publisher
      朝倉書店
    • Related Report
      2012 Annual Research Report
  • [Book] 「遺伝子・ゲノムの構造」「酢の機能と科学」酢酸菌研究会編

    • Author(s)
      東 慶直
    • Publisher
      朝倉書店
    • Related Report
      2012 Final Research Report
  • [Book] Genetic instability of Acetobacter pasteurianus revealed by whole genome analyses

    • Author(s)
      Y. Azuma
    • Publisher
      近畿大学 生物理工学部紀要
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://web.me.com/yoshinaoazuma/public_html/Welcome.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://web.me.com/yoshinaoazuma/public_html/Welcome.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 融合微生物及びその融合微生物を用いた発酵産物の製造方法2013

    • Inventor(s)
      東 慶直、波多野 裕美
    • Industrial Property Rights Holder
      学校法人近畿大学
    • Filing Date
      2013-01-08
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 融合微生物及びその融合微生物を用いた発酵産物の製造方法2013

    • Inventor(s)
      東 慶直
    • Industrial Property Rights Holder
      東 慶直
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-000843
    • Filing Date
      2013-01-08
    • Related Report
      2012 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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