Development of a system to find bioactivemolecues by genome scanning

• Project/Area Number: 22510223
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: ISHIKAWA Jun 国立感染症研究所, 生物活性物質部, 室長 (40202957)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ゲノム / 次世代シークエンサー / 抗生物質 / 応用微生物 / 創薬 / 生物活性物質

Research Abstract

To develop a system to find bioactive molecules by genome scanning, Nocardia brasiliensis IFM 0406 was sequenced by a next-generation sequencer. Many candidates for biosynthetic gene clustres of bioactive molecules were found, and the metabolite of one of the clusters was detected by forced expression of positive regulatory genes. These results suggest that genome scanning facilitates the finding of novel bioactive molecules.

Research Products

• [Journal Article] Complete genome sequence of the motile actinomycete Actino-planes missouriensis 431^T (= NBRC 102363^T) (2012)
  • Author(s): Yamamura, H., Ohnishi, Y., Ishikawa, J., Ichikawa, N., Ikeda, H., Sekine, M., Harada, T., Horinouchi, S., Otoguro, M., Tamura, T., Suzuki, K., Hoshino, Y., Arisawa, A., Nakagawa, Y., Fujita, N., and Hayakawa, M.
  • Journal Title: Stand. Genomic Sci. Volume: 7 Pages: 294-303
  • Peer Reviewed
• [Journal Article] Identification of nocobactin NA biosynthetic gene clusters in Nocardia farcinica. (2011)
  • Author(s): Hoshino, Y., Chiba, K., Ishino, K., Fukai, T., Igarashi. Y., Yazawa, K., Mikami, Y., and Ishikawa, J.
  • Journal Title: J. Bacteriol. Volume: 193 Pages: 441-448
  • Peer Reviewed
• [Journal Article] Identification of nocobactin NA biosynthetic gene clusters in Nocardia farcinica. (2011)
  • Author(s): Hoshino, Y.
  • Journal Title: Journal of Bacteriology Volume: 193 Pages: 441-448
  • Peer Reviewed
• [Presentation] De novoアセンブリーの最適化と二次代謝生合成遺伝子発見ツールの開発 (2013)
  • Author(s): 石川淳
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] 二次代謝産物研究における次世代シークエンサーの利用 (2012)
  • Author(s): 石川淳
  • Organizer: 日本農芸化学会東北支部シンポジウム
  • Place of Presentation: 秋田
  • Year and Date: 2012-06-30
• [Presentation] 次世代シークエンサーによる「とりあえずゲノム」解析のすすめ (2012)
  • Author(s): 石川淳
  • Organizer: 第56回日本医真菌学会総会
  • Place of Presentation: 東京
• [Presentation] Nocobactin NA biosynthesis gene clusters in Nocardia farcinica (2011)
  • Author(s): Hoshino, Yasutaka
  • Organizer: 16th International Symposium on the Biology of Actinomycetes
  • Place of Presentation: Mexico
  • Year and Date: 2011-12-11
• [Presentation] Nocobactin NA biosynthesis gene clusters in Nocardia farcinica (2011)
  • Author(s): Hoshino, Y, Chiba, K., Ishino, K. and Ishikawa, J.
  • Organizer: 16th Intl. Symp. on the Biology of Actinomycetes
  • Place of Presentation: Puerto Vallarta, Mexico
• [Presentation] 次世代シークエンサーによるMicromonospora griseorubidaのゲノム解析 (2010)
  • Author(s): 石川淳
  • Organizer: 第25回日本放線菌学会大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-02