| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Skeleton of triterpene is formed from oxidosqualene by triterpene synthase. Many triterpene synthases give tetra- or penta-cyclic products, while marneral synthase and achilleol synthase give a mono-cyclic product. In this study, these two enzymes were expressed in the yeast where di-oxidosqualene was accumulated to obtain a new natural triterpene with two separate rings which was produced by cyclization of di-oxidosqualene from both ends. TLC analysis showed that the target compound was produced by the transformed yeast with squalene epoxidase and achilleol synthase genes.
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