PRODUCTION OF NEW NATURAL PRODUCTS BY BIOSYNTHETIC ENGINEERING
Project/Area Number |
22510225
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
|
Research Institution | Nigata University of Phermacy and Applied Life Sciences |
Principal Investigator |
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 生合成 / 人為的制御 / トリテルペン合成酵素 / ジオキシドスクアレン / アキレオール / マーネラル / トリテルペンサポニン / ソヤサボゲノールB / オキシドスクアレン / β-アミリン / 酵母 / 生合成の人為的制御 / 生合成工学 / 異種発現系 / β-アミリン / ソヤサポゲノールB |
Research Abstract |
Skeleton of triterpene is formed from oxidosqualene by triterpene synthase. Many triterpene synthases give tetra- or penta-cyclic products, while marneral synthase and achilleol synthase give a mono-cyclic product. In this study, these two enzymes were expressed in the yeast where di-oxidosqualene was accumulated to obtain a new natural triterpene with two separate rings which was produced by cyclization of di-oxidosqualene from both ends. TLC analysis showed that the target compound was produced by the transformed yeast with squalene epoxidase and achilleol synthase genes.
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Report
(4 results)
Research Products
(14 results)