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Exploration of chemical probes for investigation of oxidative metabolism of auxins

Research Project

Project/Area Number 22510228
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionKyoto University

Principal Investigator

MIYAGAWA Hisashi  京都大学, 大学院・農学研究科, 教授 (10219735)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsオーキシン / 異化代謝 / 質量分析 / スクリーニング / 酸化酵素 / indole-3-acetic acid / oxidation / inhibitor / screening / 植物ホルモン
Research Abstract

Indole-3-acetic acid is a class of plant hormones, which is responsible for plant growth regulation. The concentration of IAA is strictly controlled by metabolism and transport. In IAA metabolism, oxidation plays an important role, but its mechanisms are not fully understood. In this study, we developed a screening system for inhibitors of IAA oxidation. As a result, we could establish a high-throughput screening system using LC/MS/MS to detect oxidative metabolites and evaluated the action of the known inhibitor of IAA oxidation in rice.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (11 results)

All 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] Probing the role of tryptophan-derived secondary metabolism in defense responses against Bipolaris oryzae infection in rice leaves by a suicide substrate of tryptophan decarboxylase2012

    • Author(s)
      Ishihara, A; Nakao, T; Mashimo, Y; Murai, M; Ichimaru, N; Tanaka, C; Nakajima, H; Wakasa, K; Miyagawa, H
    • Journal Title

      Phytochemistry

      Volume: 72 Pages: 7-13

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of a small Peptide from combinatorial libraries that can activate the plant immune system by a jasmonic acid signaling pathway2011

    • Author(s)
      Miyashita, M; Oda, M; Ono, Y; Komoda, E; and Miyagawa, H
    • Journal Title

      ChemBioChem

      Volume: 12 Issue: 9 Pages: 1323-9

    • DOI

      10.1002/cbic.201000694

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Probing the role of tryptophan-derived secondary metabolism in defense responses against Bipolaris oryzae infection in rice leaves by a suicide substrate of tryptophan decarboxylase2011

    • Author(s)
      Ishihara, A; Nakao, T; Mashimo, Y; Murai, M; Ichimaru, N; Tanaka, C; Nakajima, H; Wakasa, K; Miygawa, H
    • Journal Title

      Phytochemistry

      Volume: 72 Pages: 7-13

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物におけるインドール-3-酪酸代謝物の分析2013

    • Author(s)
      西野 雄人、小山 彩香、大西 敦子、宮下正弘、宮川 恒
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 病原感染イネにおけるオーキシン代謝2013

    • Author(s)
      大西 敦子、田中 千尋、青木 秀之、宮下正弘、宮川 恒
    • Organizer
      日本農薬学会第38回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2013-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 病原感染イネにおけるオーキシン代謝2013

    • Author(s)
      大西 敦子、田中千尋、青木秀之、宮下正弘、宮川 恒
    • Organizer
      日本農薬学会第38回大会
    • Place of Presentation
      筑波大
    • Related Report
      2012 Annual Research Report
  • [Presentation] 植物におけるインドール-3-酪酸代謝物の分析2013

    • Author(s)
      西野 雄人、小山 彩香、大西 敦子、宮下 正弘、宮川 恒
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大
    • Related Report
      2012 Annual Research Report
  • [Presentation] ネOsSAUR51遺伝子の高発現が もたらす白葉枯病抵抗性と オーキシン量の変化について2012

    • Author(s)
      青木 秀之、大西 敦子、宮下 正弘、宮川恒、矢頭 治
    • Organizer
      第30回日本植物細胞分子生物学会大会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Year and Date
      2012-08-04
    • Related Report
      2012 Final Research Report
  • [Presentation] イネsl変異体に感染したごま葉枯病菌によるインドール-3-酢酸の生産2012

    • Author(s)
      深水 愛理沙、石原 亨、宮川 恒、中 島 廣光、若狭 暁
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] インドール酢酸のイネ植物体における酸化的代謝2011

    • Author(s)
      松田洋子、中村俊介、大西敦子、宮川恒
    • Organizer
      第29回日本植物細胞分子生物学会大会
    • Place of Presentation
      九州大学
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] イネにおけるIAAの酸化代謝2011

    • Author(s)
      松田洋子、宮川恒
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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