Self-assembly of a membrane protein governed by the fluctuation of the anchor peptide and the self-organization.

• Project/Area Number: 22510231
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Hiroshima University
• Principal Investigator: IZUMI Shunsuke 広島大学, 大学院・理学研究科, 教授 (90203116)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: アンカーペプチド / ゆらぎ / 自己組織化 / トポロジー / 膜蛋白質 / 質量分析 / 分子動力学シミュレーション / MALDI-QIT-TOF

Research Abstract

Information on the fluctuation of protein is important to elucidate the function of the penetration of proteins on the biomembrane.The interaction between phospholipid and melitin molecules as a mode pepetide was evaluated by the mass spectorometery for the liposome. As a result, the relationship between the permeability of melitin through the membrane and the temperature was well fitted by the Arrhenisu law. These experimental results suggest that melitin molecules enhances the instability of the lipid membrane due to the higher permeability which is related to the specific chemical structure.

Research Products

• [Journal Article] Hepcidin-2 is a urinary biomarker for radiation exposure in mice (2012)
  • Author(s): Iizuka, Daisuke; Kawai, Hidehiko; Yoshioka, Susumu; Izumi, Shunsuke; Nishimura, Mayumi; Shimada, Yoshiya; Suzuki, Fumio
  • Journal Title: Analytical Biochemistry Volume: 430 Pages: 179-184
  • Peer Reviewed
• [Journal Article] Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes CentrosomeMaturatio (2012)
  • Author(s): Y. Ozaki, H. Matsui, H. Asou, A. Nagamachi, D. Aki, H. Honda, S. Yasunaga,Y. Takihara, T. Yamamoto, S. Izumi
  • Journal Title: Molecular Cell Volume: 47 Pages: 694-706
  • Peer Reviewed
• [Journal Article] Alkylated Dihydroxybenzoic Acid as a MALDI Matrix Additive for Hydrophobic Peptide Analysis (2012)
  • Author(s): Y. Fukuyama, R. Tanimura, K. Maeda, M. Watanabe, S. Kawabata, S. Iwamoto,S. Izumi, K. Tanaka
  • Journal Title: Analytical Chemistry Volume: 84 Pages: 4237-4243
  • Peer Reviewed
• [Journal Article] Mode Change in the Self-Motion of a Benzoquinone Disk Coupled with NADPH System (2012)
  • Author(s): S. Nakata, Y. Matsuda, Y. Ikura, A. Takeda, S. Izumi
  • Journal Title: Chem. Phys. Chem. Volume: 13 Pages: 520-524
  • Peer Reviewed
• [Journal Article] Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturatio (2012)
  • Author(s): Y. Ozaki
  • Journal Title: Molecular Cell Volume: 47 Pages: 694-706
  • Peer Reviewed
• [Journal Article] Mode Change in the Self-Motion of a Benzoquinone Disk Coupled with NADPH System (2012)
  • Author(s): S. Nakata
  • Journal Title: Chem. Phys. Chem Volume: 13 Pages: 520-524
  • Peer Reviewed
• [Journal Article] Mode Change in the Self-Motion of a Benzoquinone Disk Coupled with a NADPH System (2012)
  • Author(s): S.Nakata, Y.Matsuda, Y.Ikura, A.Takeda, S.Izumi
  • Journal Title: Chem.Phys.Chem. Volume: 13 Issue: 2 Pages: 520-524
  • DOI: 10.1002/cphc.201100773
  • Peer Reviewed
• [Journal Article] Localized bioconvection of Euglena caused by phototaxis in the lateral direction (2011)
  • Author(s): N. Suematsu, A. Awazu, S. Izumi, S. Noda, S. Nakata, H. Nishimori
  • Journal Title: J. Phys. Soc. Jpn Volume: 80
  • Peer Reviewed
• [Journal Article] Localized bioconvection of Euglena caused by phototaxis in the lateral direction (2011)
  • Author(s): N.Suematsu, A.Awazu, S.Izumi, S.Noda, S.Nakata, H.Nishimori
  • Journal Title: J.Phys.Soc.Jpn. Volume: 80 Issue: 6 Pages: 064003-064003
  • DOI: 10.1143/jpsj.80.064003
  • Peer Reviewed
• [Journal Article] Topology and dynamics of melittin within the liposome revealed by a combination of mass spectrometry and chemical modification (2010)
  • Author(s): K. Saikusa, Y. Kono, S. Izumi
  • Journal Title: Biochem. Chiophys. Res. Commun Volume: 397 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Dynamics Study of Transmembrane Helix within Liposomes by Mass Spectrometry and Chemical Modification (2010)
  • Author(s): K. Saikusa, Y. Kono, S. Izumi
  • Journal Title: J. Mass Spectrom. Soc. Volume: 58 Pages: 75-79
  • NAID: 10026279044
  • Peer Reviewed
• [Journal Article] Topology and dynamics of melittin within the liposome revealed by a combination of mass spectrometry and chemical modification (2010)
  • Author(s): K.Saikusa, Y.Kono, S.Izumi.
  • Journal Title: Biochem.Chiophys.Res.Commun Volume: 397 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Dynamics Study of Transmembrane Helix within Liposomes by Mass Spectrometry and Chemical Modification (2010)
  • Author(s): K.Saikusa, Y.Kono, S.Izumi.
  • Journal Title: J.Mass Spectrom.Soc.Jpn Volume: 58 Pages: 75-79
  • NAID: 10026279044
  • Peer Reviewed
• [Presentation] メリチンのリン脂質膜内での非アレニウス揺らぎ (2011)
  • Author(s): 東 政行, 七種和美, 粟津暁紀, 泉 俊輔
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学(姫路)
  • Year and Date: 2011-09-17
• [Presentation] Highly sensitive MALDI analyses of hydrophobic peptides using a novel additive ADHB with conventional matrices (2011)
  • Author(s): Yuko Fukuyama, Ritsuko Tanimura,Shunsuke Izumi, Shinichi Iwamoto, Koichi Tanaka
  • Organizer: The 59th Annual Conference on Mass Spectrometry
  • Place of Presentation: Suita, Japan
  • Year and Date: 2011-09-13
• [Presentation] Highly sensitive MALDI analyses of hydrophobic peptides using a novel additive ADHB with conventional matrices (2011)
  • Author(s): Yuko Fukuyama, Ritsuko Tanimura, Shunsuke Izumi, Shinichi Iwamoto, Koichi Tanaka
  • Organizer: The 59th Annual Gonference on Mass Spectrometry
  • Place of Presentation: Suita, Japan
  • Year and Date: 2011-09-13
• [Presentation] The membrane topology and dynamics of melittin within the liposomes (2011)
  • Author(s): K. Saikusa, S. Izumi
  • Organizer: The 59th ASMS Conference
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-06-09
• [Presentation] A Mechanism of the c Ions Formation Involving the "Gly-ization" of the Thr Sidechain in Melittin in MALDI-QIT-TOF (2011)
  • Author(s): Kazumi Saikusa, Susumu Yoshioka ,Shunsuke Izumi
  • Organizer: The 59th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-05-17
• [Presentation] 膜貫通ペプチドのリン脂質膜内での非アレニウス揺らぎ (2011)
  • Author(s): 東 政行,七種和美,粟津暁紀,泉 俊輔
  • Organizer: 第3回日本生物物理学会中国四国支部大会
  • Place of Presentation: 兵庫県立大学(姫路)
  • Year and Date: 2011-05-14
• [Presentation] リパーゼの反応機構を再考する-立体選択性は何によって決まるのか? (2010)
  • Author(s): 泉俊輔
  • Organizer: BMSシンポジウム 「機能するタンパク質の構造を探る」
  • Place of Presentation: (財)微生物化学研究会 微生物化学研究所(招待講演)
  • Year and Date: 2010-12-11
• [Presentation] 蛍光検出円二色性(FDCD)を用いたタンパク質立体構造のピンポイント解析の試み (2010)
  • Author(s): 石原郁, 泉俊輔, 石田敦彦, 山崎岳, 根平達夫
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] プレゴン還元酵素の反応機構-基質特異性と反応特異性の改変 (2010)
  • Author(s): 武田智, 河野陽, 泉俊輔
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] MALDIからMALDNIVESへ-質量情報を構造情報に変換するために (2010)
  • Author(s): 泉俊輔
  • Organizer: 第51回日本生化学会 中国・四国支部例会
  • Place of Presentation: 山口大学(招待講演)
  • Year and Date: 2010-05-15
• [Presentation] ベルベノン還元酵素の反応機構-基質疎水性度と反応速度との相関 (2010)
  • Author(s): 松浦正樹, 河野陽, 山本卓, 坂本尚昭, 泉俊輔
  • Organizer: 第51回日本生化学会 中国・四国支部例会
  • Place of Presentation: 山口大学
  • Year and Date: 2010-05-15
• [Presentation] The membrane topology and dynamics of melittin within the liposomes (2010)
  • Author(s): K.Saikusa, S.Izumi
  • Organizer: The 59th ASMS Conference
  • Place of Presentation: Denver, USA
• [Patent(Industrial Property Rights)] 疎水性ペプチド様マトリックス (2013)
  • Inventor(s): 泉 俊輔
  • Industrial Property Rights Holder: 泉 俊輔
  • Filing Date: 2013-03-14
• [Patent(Industrial Property Rights)] 疎水性ペプチド様マトリックス添加剤 (2013)
  • Inventor(s): 泉 俊輔
  • Industrial Property Rights Holder: 泉 俊輔
  • Filing Date: 2013-03-04
• [Patent(Industrial Property Rights)] S-ニトロソ物質の質量分析法 (2013)
  • Inventor(s): 渡辺 真,岩本慎一,佐藤孝明,田中耕一,泉 俊輔
  • Industrial Property Rights Holder: 島津製作所,広島大学
  • Filing Date: 2013-03-13
• [Patent(Industrial Property Rights)] 疎水性ペプチド用マトリックス (2013)
  • Inventor(s): 泉 俊輔
  • Industrial Property Rights Holder: 泉 俊輔
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-03-14
• [Patent(Industrial Property Rights)] 疎水性ペプチド用マトリックス添加剤 (2013)
  • Inventor(s): 泉 俊輔
  • Industrial Property Rights Holder: 泉 俊輔
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-03-04
  • Overseas
• [Patent(Industrial Property Rights)] 特許願S-ニトロソ物質の質量分析法 (2012)
  • Inventor(s): 渡辺真, 岩本慎一, 佐藤孝明, 田中耕一, 泉俊輔
  • Industrial Property Rights Holder: (株)島津製作所, 広島大学
  • Filing Date: 2012-03-13
• [Patent(Industrial Property Rights)] 特許願質量分析用マトリックスの添加剤 (2011)
  • Inventor(s): 福山裕子, 泉俊輔
  • Industrial Property Rights Holder: (株)島津製作所, 広島大学
  • Filing Date: 2011-09-09