Studies on the regulatory systems for the concentrations of cytosolic heavy-metal ions those are indispensable to cyanobacterial physiology, and application of this systems to the phytoremediation.

• Project/Area Number: 22510234
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Ehime University
• Principal Investigator: MORITA Hayato 愛媛大学, 農学部, 准教授 (50274303)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Hidenori 愛媛大学, 無細胞生命工学研究センター, 教授 (60124682)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 生体機能関連物質 / 細胞内重金属イオン濃度制御 / 藍色細菌 / 重金属ストレス耐性 / 転写調節 / SmtB / 重金属イオンセンシング / ゲルNMR / ゲルシフトアッセイ

Research Abstract

Interrelationships between the structures and the functions of SmtB family proteins, those are bifunctional proteinsas the sensor for cytosolic heavy metal ion concentration and as the transctiptional factrors, are cleared, and the way to design the genetically modified SmtB family proteins to adjust the tolerance of cyanobacterial cells to the heavy metal ion stress is developed.

Research Products

• [Journal Article] Spatio-temporal expression of PsAPY1 during germination, defferentiation, and organogenesis in pea (Pisum sativum L. Var. Alaska) (2013)
  • Author(s): Trivima T. Sahi V.P., Morita E.H., Abe S.
  • Journal Title: Acta Physiol. Plant Volume: 35 Issue: 4 Pages: 1269-1279
  • DOI: 10.1007/s11738-012-1166-9
  • Peer Reviewed
• [Journal Article] MKRN expression pattern during embryonic and post-embryonic organogenesis in rice (Oryza sativa L. var. Nipponbare) (2013)
  • Author(s): Wadekar H.B., Sahi V.P., Morita E.H., Abe S.
  • Journal Title: Planta Volume: 237 Issue: 4 Pages: 1083-1095
  • DOI: 10.1007/s00425-012-1828-2
  • Peer Reviewed
• [Journal Article] Comparative study of the different mechanisms for zinc ion stress sensing in two cyanobacterial strains, <i>Synechococcus</i> sp. PCC 7942 and <i>Synechocystis</i> sp. PCC 6803 (2012)
  • Author(s): Morita EH, Kawamoto S, Abe S, Nishiyama Y, Ikegami T, Hayashi H.
  • Journal Title: BIOPHYSICS Volume: 8 Issue: 0 Pages: 103-109
  • DOI: 10.2142/biophysics.8.103
  • NAID: 130002061591
  • ISSN: 1349-2942
  • Peer Reviewed
• [Journal Article] Elongation factor G Is a critical target during oxidative damage to the translation system of Escherichia coli. (2012)
  • Author(s): Nagano T, Kojima K, Hisabori T, Hayashi H, Morita EH, Kanamori T, Miyagi T, Ueda T, Nishiyama Y.
  • Journal Title: J. Biol. Chem. Volume: 287 (34) Pages: 28697-28704
• [Journal Article] Elongation Factor G Is a Critical Target during Oxidative Damage to the Translation System of Escherichia coli. (2012)
  • Author(s): Nagano T, Kojima K, Hisabori T, Hayashi H, Morita EH, Kanamori T, Miyagi T, Ueda T, Nishiyama Y.
  • Journal Title: J. Biol. Chem. Volume: 287(34) Issue: 34 Pages: 28697-28704
  • DOI: 10.1074/jbc.m112.378067
  • Peer Reviewed
• [Journal Article] Distinct features of protein folding by the GroEL system from a psychrophilic bacterium, Colwellia psychrerythraea 34H (2012)
  • Author(s): Yamauchi S, Ueda Y, Matsumoto M, Inoue U, Hayashi H.
  • Journal Title: Extremophiles Volume: 16 Issue: 6 Pages: 871-882
  • DOI: 10.1007/s00792-012-0483-7
  • Peer Reviewed
• [Journal Article] A molecular insight into Darwin's 'plant brain hypothesis' through expression pattern study of the MKRN gene in plant embryo compared with mouse embryo (2012)
  • Author(s): Sahi VP, Wadekar HB, Ravi NS, Arumugam TU, Morita EH, Abe S
  • Journal Title: Plant Signaling and Behavior Volume: 7(3) Issue: 3 Pages: 375-381
  • DOI: 10.4161/psb.19094
  • Peer Reviewed
• [Journal Article] Comparative study of the different mechanisms for zinc ion stress sensing in two cyanobacterial strains, Synechococcus sp.PCC 7942 and Synechocystis sp.PCC 6803 (2012)
  • Author(s): Morita EH, Kawamoto S, Abe S, Nishiyama Y, Ikegami T, Hayashi H
  • Journal Title: Biophysics Volume: (In press)(未定)
  • Peer Reviewed
• [Journal Article] Expression of a gene encoding a MKRN RING zinc finger protein in early germination stages of pea (Pisum sativum L.Var.Alaska) and its possible role in differentiation (2011)
  • Author(s): V.P.Sahi, T.U.Arumugam, E.H.Morita, S.Abe
  • Journal Title: Acta Physiol.Plant. Volume: (In press)
  • Peer Reviewed
• [Presentation] Structural analysis of the molecular mechanism for the resistance to heavy-metal ion stresses in cyanobacteria. (2013)
  • Author(s): E.H. Morita, MS. Rahul, H. Hayashi, T. Ikegami, S. Abe
  • Organizer: 第54回植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] Molecular insights into the different mechanisms of heavy-metal ion sensing in cyanobacterium, Synechococcus sp. PCC 7942. (2013)
  • Author(s): 9M.S. Rahul, H, Hayashi, S, Abe, E.H. Morita
  • Organizer: 第54回植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] Structural and functional analysis of the C-terminal region of telomere repeat-binding protein I from Arabidopsis thaliana (AtTRP1) for telomere DNA sequence recognition. (2013)
  • Author(s): A.Ochi, S. Abe, E.H. Morita
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Molecular insights into the different mechanisms of heavy-metal ion sensing in cyanobacterium, Synechococcus sp. PCC 7942. (2013)
  • Author(s): M.S. Rahul, H, Hayashi, S, Abe, E.H. Morita
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Structural analysis of the molecular mechanism for the resistance to heavy-metal ion stresses in cyanobacteria. (2013)
  • Author(s): E.H. Morita, MS. Rahul, H. Hayashi, T. Ikegami, S. Abe
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Expression of APY1 During Germination, Differentiation and Organogenesis in Pea (Pisum sativum L.Var.Alaska) (2012)
  • Author(s): Trivima S, Yoneda M Sahi VP, Morita EH, Abe S
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] Molecular mechanism of cytosolic Zn2+ concentration sensing by a cyanobacterial transcription factor SmtB from Synechococcus sp. PCC 7942. (2012)
  • Author(s): Morita EH, Abe S,Ikegami T,Hayashi H.
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] Molecular mechanism of cytosolic Zn^<2+> concentration sensing by a cyano-bacterial transcription factor SmtB from Synechococcus sp.PCC 7942 (2012)
  • Author(s): Morita EH, Abe S, Ikegami T, Hayashi H
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] Expression studies of MKRN gene reveals spatially similar patterns in functionally and anatomically analogous regions of plant andmouse embryos (2012)
  • Author(s): Sahi VP, Hanumant BW, Suganthan NR, Morita EH, Abe S
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] Functional Analysis of the N-terminal Sequence of SmtB, the Transcription Repressor for the Heavy-Metal Ion Stress Induced Metallo- thionein Like Protein Expression, in cyano- baterium, Synechococcussp. PCC 7942 (2012)
  • Author(s): Rahul MS,Hayashi H, Abe S,Morita EH
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] MKRN Expression Pattern in Apical-Basal and Eadial Axis of Development in Rice (Oryza sativa L.var.Nipponbare) (2012)
  • Author(s): Hanumant BW, Sahi VP, Morita EH, AbeS
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Functional Analysis of the N-terminal Sequence of SmtB, the Transcription Repressor for the Heavy-Metal Ion Stress Induced Metallothionein Like Protein Expression, in Cyanobaterium, Synechococcus sp.PCC 7942 (2012)
  • Author(s): Rahul MS, Hayashi H, Abe S, Morita EH
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Arabidopsis thalianaテロメア結合タンパク質(AtTRP1)C末端領域のテロメアDNA配列結合活性における構造機能相関解析 (2012)
  • Author(s): 越智ありさ, 阿部俊之助, 森田勇人
  • Organizer: 第53回植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Structural and functional analysis of C-terminal region of telomeric repeat-binding protein I from Arabidopsis thaliana (AtTRP1) with EMSA (2012)
  • Author(s): A.OChi, S. Abe, E.H. Morita
  • Organizer: The 10 th Matsuyama Internationla Symposium on Cell-Free Sciences
  • Place of Presentation: 松山全日空ホテル
• [Presentation] Proteins responsible for salt tolerance in Finger Millet and Rice (2011)
  • Author(s): Kishor AS, M.Raveendran, D.Vijayalakshmi, A.Nirmalakumari, Abe S, Morita EH
  • Organizer: プロテイン・アイランド・松山国際シンポジウム2011
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2011-09-22
• [Presentation] Structural and functional analysis of C-terminal region of telomeric repeat-binding protein 1 from Arabidopsis thaliana (AtTRP1) for telomere DNA sequence recognition (2011)
  • Author(s): Ochi A, Abe S, Morita EH
  • Organizer: プロテイン・アイランド・松山国際シンポジウム2011
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2011-09-22
• [Presentation] Spectroscopic and biochemical analyses for the molecular mechanism of cytosolic zinc ion concentration sensing by cyanobacterial transcriptional factors, SmtB family. (2010)
  • Author(s): 森田勇人、阿部俊之助、池上貴久、林秀則
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Spectroscopic and biochemical analyses for the molecular mechanism of cytosolic zinc ion concentration sensing by cyano- bacterial transcriptional factors, SmtB family. (2010)
  • Author(s): 森田勇人、阿部俊之助、池上貴久、林秀則
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-21
• [Presentation] Newly developed physical chemistry of life with THz spectroscopy. (2010)
  • Author(s): 森田勇人、西澤誠治
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(招待講演)
  • Year and Date: 2010-09-21
• [Remarks] 現在専用ホームページの作成中(平成 25年 10 月に公開予定)