Nitrogen dynamics in geo- and hydro-thermal environments elucidated from isotope geochemistry and microbiology

• Project/Area Number: 22540499
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Geochemistry/Astrochemistry
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NISHIZAWA Manabu 独立行政法人海洋研究開発機構, システム地球ラボ, 研究員 (60447539)
• Co-Investigator(Kenkyū-buntansha): TAKAI Ken 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクタ (80359166)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生物地球化学 / 熱水域 / 窒素動態 / 微生物 / 同位体 / 環境遺伝子 / 熱水系 / 微生物相 / 窒素同位体 / 環境DNA

Research Abstract

This study first showed that ammonia oxidation by thermophilic archaea mainly provided chemical energy for the development of microbial community in an ammonia-rich hot spring. In contrast, culture experiments first suggest that nitrogen fixation by hyperthermophilic methanogen is a key process to regulate primary production of subsurface microbial communities in ammonia-poor hydrothermal environments.

Research Products

• [Journal Article] Nitrification-driven forms of nitrogen metabolism in microbial mat communities thriving along an ammonium-enriched subsurface geothermal stream (2013)
  • Author(s): Nishizawa, M., Koba, K., Makabe, A., Yoshida, N., Kaneko, M., Hirao, S., Ishibashi, J., Yamanaka, T., Shibuya, T., Kikuchi, T., Hirai, M., Miyazaki, J., Nunoura, T., Takai, K
  • Journal Title: Geochim. Cosmochim. Acta Volume: 113 Pages: 152-173
  • Peer Reviewed
• [Journal Article] Molecular biological and isotopic biogeochemical prognoses of the nitrification-driven dynamic microbial nitrogen cycle in hadopelagic sediments (2013)
  • Author(s): Nunoura, T., Nishizawa, M., Kikuchi, T., Tsubouchi, T., Hirai, M., Koide, O., Miyazaki, J., Hirayama, H., Koba, K., Takai, K
  • Journal Title: Environmental Microbiology Volume: - Issue: 11 Pages: 3087-3107
  • DOI: 10.1111/1462-2920.12152
  • Peer Reviewed
• [Journal Article] IODP Expedition 331: Strong and Expansive Subseafloor Hydrothermal Activities in the Okinawa Trough (2012)
  • Author(s): Takai, K., Mottl, M. J., Nielsen, S.H.H
  • Journal Title: Scientific Drilling Volume: 13 Issue: 13, April 2012 Pages: 19-27
  • DOI: 10.2204/iodp.sd.13.03.2011
• [Journal Article] Cell-Specific Thioautotrophic Productivity of Epsilon-Proteobacterial Epibionts Associated with Shinkaia crosnieri. (2012)
  • Author(s): Watsuji, T., Nishizawa, M., Morono, Y. et al.,
  • Journal Title: PLoS ONE Volume: - Issue: 10 Pages: e46282-e46282
  • DOI: 10.1371/journal.pone.0046282
  • Peer Reviewed
• [Journal Article] In situ iron isotope analyses of pyrite and organic carbon isotope ratios in the Fortescue Group: Metabolic variations of a Late Archean ecosystem. (2012)
  • Author(s): Yoshiya, K., Nishizawa, M., Sawaki, Y. et al.,
  • Journal Title: Precambrian Research Volume: 212-213 Pages: 169-193
  • DOI: 10.1016/j.precamres.2012.05.003
  • Peer Reviewed
• [Journal Article] Domain-level identification and quantification of relative prokaryotic cell abundance in microbial communities by Micro-FTIR spectroscopy (2012)
  • Author(s): Motoko Igisu
  • Journal Title: Environmental Microbiology Report Volume: 4 Issue: 1 Pages: 42-49
  • DOI: 10.1111/j.1758-2229.2011.00277.x
  • Peer Reviewed
• [Journal Article] Carbon and nitrogen assimilation in deep subseafloor microbial cells (2011)
  • Author(s): Morono, Y., Terada, T., Nishizawa, M., Ito, M., Hillion, F., Takahata, N., Sano, Y., Inagaki, F
  • Journal Title: Proc. National Aca. Sci. USA Volume: 108 Pages: 18295-18300
  • Peer Reviewed
• [Journal Article] Carbon and nitrogen assimilation in deep subseafloor microbial cells (2011)
  • Author(s): Morono, Y., Terada, T., Nishizawa, M., et al
  • Journal Title: Proc.National Aca.Sci.USA Volume: 108 Issue: 45 Pages: 18295-18300
  • DOI: 10.1073/pnas.1107763108
  • Peer Reviewed
• [Journal Article] Integrated Ocean Drilling Program Expedition 331 Preliminary Report (2010)
  • Author(s): Expedition 331 Scientists
  • Journal Title: Deep Hot Biosphere
  • DOI: 10.2204/iodp.pr.331.2010
  • Peer Reviewed
• [Journal Article] 地下熱水系における微生物地球化学:菱刈金山地下熱水系の研究例 (2010)
  • Author(s): 西澤学
  • Journal Title: 地球化学 Volume: 44 Pages: 127-136
  • NAID: 110009327691
  • Peer Reviewed
• [Journal Article] Deep Hot Biosphere, 1 September-30 October 2010 (2010)
  • Author(s): Expedition 331 Scientists
  • Journal Title: Integrated Ocean Drilling Program Expedition 331 Preliminary Report Pages: 1-63
  • Peer Reviewed
• [Presentation] Compositional and isotopic properties of nitrogen in subseafloor hydrothermal environments at the Iheya North field in the mid-Okinawa Trough (2012)
  • Author(s): Nishizawa, M., Takai, K
  • Organizer: AGU Fall meeting 2012
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-03
• [Presentation] Chemical and isotopic compositions of sulfur and nitrogen in subseafloor hydrothermal environments at the Iheya North field in the mid-Okinawa Trough (2012)
  • Author(s): Nishizawa, M., Aoyama, S., Ueno, Y., Takai, K
  • Organizer: IODP331 2nd Post-cruise meeting
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2012-11-12
• [Presentation] 海溝堆積物における窒素動態と微生物生態 (2012)
  • Author(s): 西澤学、布浦拓郎、平井美穂、真壁明子、柳川勝紀、野牧秀隆、北橋倫、木庭啓介、高井研
  • Organizer: 第4回日韓微生物生態シンポジウム
  • Place of Presentation: 豊橋技術科学大学
  • Year and Date: 2012-09-20
• [Presentation] 菱刈金山地下温泉小川に発達した微生物生態と窒素動態 (2011)
  • Author(s): 西澤学
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学北部キャンパス
  • Year and Date: 2011-10-08
• [Presentation] 地球化学と微生物学の結合による生元素循環の進化に関する研究 (2011)
  • Author(s): 西澤学
  • Organizer: 2011年度日本地球化学会年会
  • Place of Presentation: 北海道大学札幌キャンパス(奨励賞受賞講演)(招待講演)
  • Year and Date: 2011-09-15
• [Presentation] 超好熱メタン生成菌の窒素固定:金属要求性と窒素同位体システマティクス (2011)
  • Author(s): 西澤学、宮崎淳一、木庭啓介、高井研
  • Organizer: 2011年度日本地球化学会年会
  • Place of Presentation: 北海道大学札幌キャンパス
  • Year and Date: 2011-09-14
• [Presentation] Linking nitrogen isotope systematics and microbiology in a subsurface geothermal water stream (2011)
  • Author(s): Nishizawa, M., Koba, K., Makabe, A., Yoshida, N., Hirai, M., Miyazaki, J., Nunoura, T., Takai, K
  • Organizer: Hishikari Gold mine, Japan. Goldschmidt2011
  • Place of Presentation: Prague, Czech
  • Year and Date: 2011-08-15
• [Presentation] Linking nitrogen isotope systematics and microbiology in a subsurface geothermal water stream, Hishikari Gold mine, Japan (2011)
  • Author(s): Nishizawa, M.
  • Organizer: Goldschmidt2011
  • Place of Presentation: Congress center, Prague, Czech
  • Year and Date: 2011-08-15
• [Presentation] 菱刈金山地下熱水系に発達した微生物マットの生物地球化学 (2010)
  • Author(s): 西澤学
  • Organizer: 日本地球惑星科学連合2010年度連合大会
  • Place of Presentation: 幕張メッセ、幕張
  • Year and Date: 2010-05-23
• [Presentation] Isotopic effects and kinetics of nitrogen fixation by hyperthermophilic methanogen: Insight into nitrogen biogeochemistry in the contemporary and ancient hydrothermal ecosystem (2010)
  • Author(s): Nishizawa, M., Miyazaki, J., Takai, K
  • Organizer: Astrobiology Science Conference 2010 (AbSciCon 2010)
  • Place of Presentation: League City, Texas, USA
  • Year and Date: 2010-04-26
• [Presentation] Isotopic effects and kinetics of nitrogen fixation by hyperthermophilic me thanogen : Insight into nitrogen biogeochemistry in the contemporary and ancient hydrothermal ecosystem (2010)
  • Author(s): Nishizawa, M.
  • Organizer: Astrobiology Science Conference 2010
  • Place of Presentation: Conference center, League City, Texas, USA
  • Year and Date: 2010-04-26
• [Presentation] 海溝堆積物における窒素動態と微生物生態
  • Author(s): 西澤学
  • Organizer: 第４回日韓微生物生態シンポジウム
  • Place of Presentation: 豊橋技術科学大学
  • Invited
• [Presentation] 超深海堆積物におけるアンモニア酸化と脱窒
  • Author(s): 西澤学
  • Organizer: 2012年度日本地球化学会年会
  • Place of Presentation: 九州大学箱崎キャンパス
• [Presentation] Chemical and isotopic compositions of sulfur and nitrogen in subseafloor hydrothermal environments at the Iheya North field in the mid-Okinawa Trough
  • Author(s): Nishizawa, M.
  • Organizer: IODP331 2nd Post-cruise meeting
  • Place of Presentation: Hawaii, USA
• [Presentation] Compositional and isotopic properties of nitrogen in subseafloor hydrothermal environments at the Iheya North field in the mid-Okinawa Trough.
  • Author(s): Nishizawa, M.
  • Organizer: AGU Fall meeting 2012
  • Place of Presentation: San Francisco, USA