Development of HPLC separation system of bio-based highly hydrophilic compounds with ultrahigh separation efficiencies.

• Project/Area Number: 22550074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: IKEGAMI Tohru 京都工芸繊維大学, 工芸科学研究科, 准教授 (20301252)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: HPLC / シリカモノリス / 重合修飾 / 高親水性化合物 / 高性能分離 / ＨＰＬＣ

Research Abstract

Characterization method for hydrophilic interaction chromatographic stationary phases, based on hydrophilicity and selectivity for partial structure of analytes was developed. Using radical polymerization, functionalization method of monolithic silica columns was established. We could increase hydrophilic retention of highly polar compounds, by controlling monomer concentration of polymerization process. Significantly higher hydrophilicity and higher separation efficiency compared to commercially available HPLC columns were realized.

Research Products

• [Journal Article] Estimation and optimization of the peak capacity of one-dimensional gradient high performance liquid chromatography using a long monolithic silica capillary column (2012)
  • Author(s): K. Horie, Y. Sato, T. Kimura, T. Nakamura, Y. Ishihama, Y. Oda, T. Ikegami, and N. Tanaka
  • Journal Title: J Chromatogr A Volume: 1228 Pages: 283-91
  • DOI: 10.1016/j.chroma.2011.12.088
  • Peer Reviewed
• [Journal Article] New silica monolith bonded chiral (R)-r butyrolactone for enantioselective micro high-performance liquid chromatography (2011)
  • Author(s): Ghanem, Ashraf; Ikegami, Tohru; Tanaka, Nobuo
  • Journal Title: Chirality Volume: 23(10) Issue: 10 Pages: 887-890
  • DOI: 10.1002/chir.21004
  • Peer Reviewed
• [Journal Article] Recent advances in silica-based monoliths: Preparations characterizations and applications (2011)
  • Author(s): Ghanem, Ashraf; Ikegami, Tohru
  • Journal Title: Journal of Separation Science Volume: 34(16-17) Issue: 16-17 Pages: 1945-1957
  • DOI: 10.1002/jssc.201100086
  • Peer Reviewed
• [Journal Article] Chromatographic characterization of hydrophilic interaction liquid chromatography stationary phases: Hydrophilicity, charge effects, structural selectivity, and separation efficiency (2011)
  • Author(s): Kawachi, Yuusuke; Ikegami, Tohru; Takubo, Hirotaka; Ikegami, Yuka; Miyamoto, Masatoshi; Tanaka, Nobuo
  • Journal Title: Journal of Chromatography, A Volume: 1218(35) Issue: 35 Pages: 5903-5919
  • DOI: 10.1016/j.chroma.2011.06.048
  • Peer Reviewed
• [Journal Article] Monolithic silica rod columns for high-efficiency reversed-phase liquid chromatography (2011)
  • Author(s): Miyazaki, Shota; Takahashi, Masakazu; Ohira, Masayoshi; Terashima, Hiroyuki; Morisato, Kei; Nakanishi, Kazuki; Ikegami, Tohru; Miyabe, Kanji; Tanaka, Nobuo
  • Journal Title: Journal of Chromatography, A Volume: 1218(15) Issue: 15 Pages: 1988-1994
  • DOI: 10.1016/j.chroma.2010.11.032
  • Peer Reviewed
• [Journal Article] Mixed-mode monolithic silica as a chromatographic separation medium (2011)
  • Author(s): Jemale, Alia Sofie; Jaafar, Jafariah; Watanabe, Yuta; Ikegami, Tohru; Tanaka, Nobuo
  • Journal Title: Journal of Liquid Chromatography & Related Technologies Volume: 34(7) Issue: 7 Pages: 500-510
  • DOI: 10.1080/10826076.2011.556969
  • Peer Reviewed
• [Journal Article] Monolithic silica rod columns for high-efficiency reversed-phase liquid chromatography. (2011)
  • Author(s): S.Miyazaki, 他
  • Journal Title: J.Chromatogr.A Volume: 1218 Pages: 1988-1994
  • Peer Reviewed
• [Journal Article] One-Dimensional Capillary Liquid Chromatographic Separation Coupled with Tandem Mass Spectrometry Unveils the Escherichia coli Proteome on a Microarray Scale (2010)
  • Author(s): M. Iwasaki, S. Miwa, T. Ikegami, M. Tomita, N. Tanaka, and Y. Ishihama
  • Journal Title: Anal Chem Volume: 82 Issue: 7 Pages: 2616-20
  • DOI: 10.1021/ac100343q
  • Peer Reviewed
• [Journal Article] One-dimensional capillary liquid chromatographic separation coupled with tandem mass spectrometry unveils the Escherichia coli proteome on a microarray scale. (2010)
  • Author(s): M.Iwasaki, 他
  • Journal Title: Anal.Chem. Volume: 82 Pages: 2616-2620
  • Peer Reviewed
• [Presentation] Monolithic silica columns for HPLC separation media (2012)
  • Author(s): Tohru Ikegami
  • Organizer: International Symposium on Functional Materials Based on Silicon-Oxygen Systems (招待講演)
  • Place of Presentation: Tokyo
  • Year and Date: 2012-09-25
• [Presentation] Preparation and Characterization of HILIC Type of Monolithic Silica Columns (2012)
  • Author(s): Tohru Ikegami, Yuusuke Kawachi, Nobuo Tanaka
  • Organizer: 38th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Anaheim, CA, USA
  • Year and Date: 2012-06-20
• [Presentation] アクリルアミドを重合したHILIC型固定相の開発と分離特性 (2012)
  • Author(s): 池上 亨,伊東和輝,宮本真敏
  • Organizer: 第19回クロマトグラフィーシンポジウム
  • Place of Presentation: 八王子市
  • Year and Date: 2012-05-25
• [Presentation] アクリルアミドを重合したHILIC型固定相の開発と分離特性 (2012)
  • Author(s): 池上 亨，伊東和輝，宮本真敏
  • Organizer: 第19回 クロマトグラフィーシンポジウム
  • Place of Presentation: 八王子
• [Presentation] Tohru Ikegami, Yuusuke Kawachi, Nobuo Tanaka (2012)
  • Author(s): Preparation and Characterization of HILIC Type of Monolithic Silica Columns
  • Organizer: 38th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Anaheim, CA, USA
• [Presentation] Monolithic silica columns for HPLC separation media (2012)
  • Author(s): Tohru Ikegami
  • Organizer: International Symposium on Functional Materials Based on Silicon-Oxygen Systems
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] HILIC 型シリカモノリスカラムの調製と再現性評価 (2011)
  • Author(s): 池上 亨
  • Organizer: 第22回クロマトグラフィー科学会議
  • Place of Presentation: 仙台市
  • Year and Date: 2011-10-21
• [Presentation] HILIC型シリカモノリスカラムの調製と再現性評価 (2011)
  • Author(s): 池上亨, 他
  • Organizer: 第22回クロマトグラフィー科学会議
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-10-21
• [Presentation] Study of retention mechanism in HILIC in terms of deuterium isotope effect (2011)
  • Author(s): Tomoyuki Okada, Tohru Ikegami, Nobuo Tanaka
  • Organizer: 36th International Symposium on High-Performance Liquid Phase Separation and Related Techniques
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2011-06-22
• [Presentation] Study of retention mechanism in HILIC in terms of deuterium isotope effect (2011)
  • Author(s): Tomoyuki Okada, 他
  • Organizer: 36^<th> International Symposium on High-Performance Liquid Phase Separation and Related Techniques
  • Place of Presentation: Budapest Congress & World Trade Center, Budapest, Hungary
  • Year and Date: 2011-06-22
• [Presentation] New Test Method for Chromatographic Characterization of Hydrophilic Interaction Chromat ography (HILIC) Stationary Phases (2011)
  • Author(s): Tohru Ikegami, Yusuke Kawachi, Hirotaka Takubo, Masatoshi Miyamoto, Nobuo Tanaka
  • Organizer: 36th International Symposium on High-Performance Liquid Phase Separation and Related Techniques
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2011-06-20
• [Presentation] New Test Method for Chromatographic Characterization of Hydrophilic Interaction Chromatography (HILIC) Stationary Phases (2011)
  • Author(s): Ikegami Tohru, 他
  • Organizer: 36^<th> International Symposium on High-Performance Liquid Phase Separation and Related Techniques
  • Place of Presentation: Budapest Congress & World Trade Center, Budapest, Hungary
  • Year and Date: 2011-06-20
• [Presentation] HILIC型シリカモノリスカラムの調製と特性評価 (2011)
  • Author(s): 池上 亨,河内佑介,田窪宏貴,宮本真敏,田中信男
  • Organizer: 第18回クロマトグラフィーシンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 2011-06-03
• [Presentation] HILIC型シリカモノリスカラムの調製と特性評価 (2011)
  • Author(s): 池上亨, 他
  • Organizer: 第18回クロマトグラフィーシンポジウム
  • Place of Presentation: 福岡大学(福岡市)
  • Year and Date: 2011-06-03
• [Presentation] HILICカラムのテスト法開発 (2010)
  • Author(s): 池上亨
  • Organizer: 第21回クロマトグラフィー科学会議
  • Place of Presentation: 西宮市 武庫川女子大学
  • Year and Date: 2010-10-23
• [Book] Monolithic Silicas in Separation Science (2011)
  • Author(s): Oscar Nunez and Tohru Ikegami
  • Publisher: ed. Wiley-VCH
• [Book] Monolithic Silicas in Separation Science (2011)
  • Author(s): Takeshi Hara, Kosuke Miyamoto, Satoshi Makino, Shohei Miwa, Tohru Ikegami, Masayoshi Ohira, Nobuo Tanaka
  • Publisher: High-speed and high-efficiency separations by utilizing monolithic silica capillary columns.
• [Book] Preparation and properties of various types of monolithic silica stationary phases for reversed-phase, hydrophilic interaction, and ion-exchange chromatography based on polymer-coated materials. (2011)
  • Author(s): O.Nunez, T.Ikegami
  • Total Pages: 11
  • Publisher: In "Monolithic Silicas in Separation Science", K.K.Unger, N.Tanaka, E.Machtejevas ed.Wiley-VCH
• [Book] High-speed and high-efficiency separations by utilizing monolithic silica capillary columns. (2011)
  • Author(s): T.Hara, 他
  • Total Pages: 24
  • Publisher: In "Monolithic Silicas in Separation Science", K.K.Unger, N.Tanaka, E.Machtejevas ed.Wiley-VCH