| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
An immunoassay technique has been widely used for many analytical fields such as diagnosis of influenza virus as well as environmental assessment and safety of foods. In such cases, immunoreactions with an antigenand an antibody have been utilizedfor highly selective analysis. However since there need many tedious and time-consuming protocols, a more rapid and simpler analytical technique is desired. In addition, in some cases, a method for labeling an enzyme to an antibody or an antigen is used for enhancement of the sensitivity of an immunoassay, but a much sensitive analytical technique would be expected. Inthis work, in order to enhance the rapidity of analysis and the sensitivity of the immunoassay, we tried to use magnetic microbeads for a support of the immunoassay and to enhance the analytical signals by using an element of piezoelectric device for detection of magnetic microbeads by applying outer magnetic fields. We have developed an immobilization method of an antibody to the microbeads and clarified the relationship between the strength of the magnetic fields and the signal of piezoelectric device. As a result a new immunoassay method has been proposed by using the present technique as the model analyte of immunoglobulin.
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