Development of fast and multi-functional biological analysis systembased on the ultra-micro droplet reaction place
Project/Area Number |
22550079
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Analytical chemistry
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
UCHIYAMA Katsumi 首都大学東京, 都市環境科学研究科, 教授 (40151899)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | マイクロチップ / 微少液滴 / 生化学分析 / インクジェット / マイクロ・ナノビーズ / 微少液滴反応 / ナノ粒子 / フォトニックバンド / 酵素免疫測定法 / ナノバイオ / マイクロ・ナノデバイス / フォトニック結晶 |
Research Abstract |
Micro-droplet reaction place was successfully developed by using ink-jet. Generation method of droplet from ink-jet was extensively analyzed and finally we have found the optimal conditions. By using droplet reaction system, size effect, which is the most important characteristics of micro-total analysis system, was realized. Using this droplet reaction system, measuring system for bio-samples using enzyme linked immune solvent assay was realized. The system for sample dispensing and measuring system for incident light fluorescent and chemiluminescence was developed. Then, three dimensional ordered structure with micro and nano beads was successfully developed and those structure were used for the novel analytical tool. ELISA using three dimensional ordered micro beads structure showed drastic increase in the detection sensitivity with 1/1000 reagent consumption when it was used as a reaction place. Finally, we have successfully fabricated photonic crystals with nano-bead and showed the possibility of material sensing by eyes.
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Report
(4 results)
Research Products
(95 results)
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[Journal Article] An integrated enzyme-linked immunosorbent assay system with an organic light-emitting diode and a chargecoupled device for fluorescence detection2011
Author(s)
Hizuru Nakajima, Yukiko Okuma, Kazuhiri Morioka, Mayo Miyake, Akihide Hemmi, Tatsuya Tobita, Masayuki yahiro, Daisuke Yokoyama, Chihaya Adachi, Nobuaki Soh, Koji Nakano, Shuhua Xue, Hulie Zeng, Katsumi Uchiyama, Toshihiko Imato
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Journal Title
Journal of Separation Science
Volume: 34
Pages: 2906-2912
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Peer Reviewed
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[Presentation] A surface plasmon resonance sensor on a compact disk-like microfluidic device2011
Author(s)
Akihide Hemmi, Tatsuya Tobita, Takashi Usui, Akihiro Moto, NobuakiSoh, Koji Nakano, Toshihiko Imato, Katsumi Uchiyama, Hizuru Nakajima
Organizer
Pittcon 2011, Atlanta
Place of Presentation
Georgia, USA
Year and Date
2011-03-13
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[Presentation] An integrated fluorescence detection system using organic light emitting diodes as light source2010
Author(s)
Mayo Miyake, Yukiko Okuma, Akihide Hemmi, Daisuke Yokoyama, Masayuki Yahiro, Chihaya Adachi, Toshihiko Imato, Katsumi Uchiyama, Hizuru Nakajima
Organizer
Pacifichem 2010
Place of Presentation
Honolulu, Hawaii, USA
Year and Date
2010-12-15
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[Presentation] 微小液滴による迅速気液平衡の達成と分析化学的応用2010
Author(s)
金子 裕司, 清野 信子, 中嶋 秀, 内山 一美
Organizer
第77回日本分析化学会有機微量分析研究懇談会第83回計測自動制御学会力学量計測部会第27回合同シンポジウム
Place of Presentation
京都大学百周年時計台記念館
Year and Date
2010-05-14
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[Book] 基礎教育シリーズ,善木 道雄,寺前 紀夫,中釜 達朗,平山 和雄,三浦 恭之,南沢 宏明,森田 孝節分析化学〈基礎編〉2011
Author(s)
本水 昌二,磯崎 昭徳,櫻川 昭雄,井原 敏博,内山 一美,善木 道雄,寺前 紀夫,中釜 達朗,平山 和雄,三浦 恭之,南沢 宏明,森田 孝節
Total Pages
191
Publisher
株式会社東京教学社
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