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Evaluation of cultured cell-activity by quartz crystal sensors for screening ofanti-cancer reagents

Research Project

Project/Area Number 22550083
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionTokyo University of Technology

Principal Investigator

MURAMATSU Hiroshi  東京工科大学, 応用生物学部, 教授 (20373045)

Co-Investigator(Renkei-kenkyūsha) KANG Hyen-wook  東京工科大学, 片柳研究所, 研究員 (40572392)
Research Collaborator 山本 裕二  セイコー・イージーアンドジー株式会社, 営業部, 主任
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords抗ガン剤 / スクリーニング / 水晶振動子センサー / 培養細胞 / 共振周波数 / 共振抵抗
Research Abstract

We have developed a monitoring system of cultured cells using quartz crystal sensors forevaluating anti-cancer reagents and studied conditions for the evaluation. The developmentsystem includes 8 channels of quartz crystal sensors and micro cameras for monitoring the cellactivity. We have decided the cell concentration applying for the evaluation and culturing timebefore the anti-cancer reagent injection, and a surface preparation method for cell adhesion.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2013 2012 2011 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (14 results)

  • [Journal Article] Young-Soo Kwon Influence of Diquat on Growth and Death of HepG2 Cells using Quartz Crystal and Micro CCD Camera2011

    • Author(s)
      Hyen-Wook Kang , Dong-Yun Lee, Hiroshi Muramatsu
    • Journal Title

      Journal of Nanoscience and Nanotechnology

      Volume: 11(5) Issue: 5 Pages: 4236-4239

    • DOI

      10.1166/jnn.2011.3660

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring of anticancer effect of cisplatin and 5-fluorouracil on HepG2 cells by quartz crystal microbalance and micro CCD camera2010

    • Author(s)
      Hyen-Wook Kang, Hiroshi Muramatsu, Burm-Jong Lee, Young-Soo Kwon
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 26 Pages: 1576-1581

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 水晶振動子センサーによる培養細胞活性評価及び細胞接着表面の最適化2013

    • Author(s)
      大谷直人,村松宏
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学・びわこ・くさつキャンパス(滋賀県)
    • Year and Date
      2013-03-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 水晶振動子センサーによる測定技術と培養細胞の状態評価への応用2012

    • Author(s)
      村松宏
    • Organizer
      BioJapan2012
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-10-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞の活性評価とその抗ガン剤評価への展開,BIOtech20122012

    • Author(s)
      村松宏
    • Organizer
      第11回国際バイオテクノロジー展
    • Place of Presentation
      東京ビッグサイト(東京都)
    • Year and Date
      2012-04-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞評価のための細胞接着表面の最適化2011

    • Author(s)
      加瀬大幹,姜顯旭,村松宏
    • Organizer
      第72回応用物理学会学術講演会
    • Place of Presentation
      山形大学(山形県)
    • Year and Date
      2011-08-31
    • Related Report
      2012 Final Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞評価のための細胞接着表面の最適化2011

    • Author(s)
      加瀬大幹, 姜顯旭, 村松宏
    • Organizer
      第72回応用物理学会学術講演会
    • Place of Presentation
      山形大学、山形市
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Monitoringfor cytoskeletal change in cultured cells underchemical stressor using QCM2010

    • Author(s)
      Hyen-Wook Kang, Hiroshi Muramatsu,Burm-Jong Lee, Young-Soo Kwon
    • Organizer
      2010International Conference on Nano Science andNano Technology
    • Place of Presentation
      GwangjuInstitute of Science and Technology, Gwangju,Korea
    • Year and Date
      2010-11-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Monitoring for cytoskeletal change in cultured cells under chemical stressor using QCM2010

    • Author(s)
      Hyen-Wook Kang, Hiroshi Muramatsu, Burm-Jong Lee, Young-Soo Kwon
    • Organizer
      2010 International Conference on Nano Science and Nano Technology
    • Place of Presentation
      Gwangju Institute of Science and Technology, Gwangju, Korea
    • Year and Date
      2010-11-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞測定のための細胞接着マトリクスの最適化2010

    • Author(s)
      加瀬大幹,姜顯旭,村松宏
    • Organizer
      第71回応用物理学会学術講演会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2010-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞測定のための細胞接着マトリクスの最適化2010

    • Author(s)
      加瀬大幹, 姜顯旭, 村松宏
    • Organizer
      第71回応用物理学会学術講演会
    • Place of Presentation
      長崎大学、長崎市
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Monitoring for intra cellular cytoskeletal changeby chemical stressors using quartz crystalmicrobalance2010

    • Author(s)
      Hyen-Wook Kang, Burm-Jong Lee,Young-Soo Kown, Hiroshi Muramatsu
    • Organizer
      The 3rd International Nano BioConference 2010
    • Place of Presentation
      Eidgenossische Technische Hochschule (ETH)Zurich, Swiss
    • Year and Date
      2010-08-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Monitoring for intra cellular cytoskeletal change by chemical stressors using quartz crystal microbalance2010

    • Author(s)
      Hyen-Wook Kang, Burm-Jong Lee, Young-Soo Kown, Hiroshi Muramatsu
    • Organizer
      The 3rd International NanoBio Conference 2010
    • Place of Presentation
      Eidgenossische Technische Hochschule (ETH) Zurich, Swiss
    • Year and Date
      2010-08-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞の活性評価とその抗ガン剤評価への展開

    • Author(s)
      村松 宏(代表者)
    • Organizer
      BIOtech2012 第11回国際バイオテクノロジー展 アカデミックフォーラム
    • Place of Presentation
      東京ビッグサイト(東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水晶振動子センサーによる測定技術と培養細胞の状態評価への応用

    • Author(s)
      村松 宏(代表者)
    • Organizer
      BioJapan 2012 出展者プレゼンテーション
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水晶振動子センサーによる培養細胞活性評価及び細胞接着表面の最適化

    • Author(s)
      大谷直人、村松 宏(代表者)
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学・びわこ・くさつキャンパス(滋賀県)
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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