Characterization of small organic molecules with threshold-energy dissociation mass spectrometry

• Project/Area Number: 22550090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKAMURA Takemichi 独立行政法人理化学研究所, 物質構造解析チーム, 専任研究員 (10360611)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 閾値エネルギー解離 / タンデム質量分析法 / 環状ペプチド化合物 / 構造解析 / モバイルプロトン / イオン移動度分析 / エネルギー分解質量分析 / 異性体識別 / 質量分析 / フラグメンテーション / 中性種脱離 / 異性化 / イオンモビリティー分析 / プロダクトイオン / イオンモビリティー / エネルギー分解

Research Abstract

The basic concept and framework of threshold-energy dissociation mass spectrometry have been applied to various tandem mass spectrometers including quadrupole-type instruments. Analysis of fragmentation behavior of some organic molecules and threshold-energies allowed us to establish the basis for the exploitation of the method for identification of small molecules including isomers. An integrated analytical strategy including threshold dissociation and ion mobility tandem mass spectrometry was shown to be promising for characterization of isomeric ions and fragmentation networks.

Research Products

• [Journal Article] Novel angiotensin I-converting enzyme inhibitory peptides found in a thermolysin-treated elastin with antihypertensive activity (2012)
  • Author(s): Sato, Y., Toyoda, T., Shimizu-Ibuka, A., Tamura, T., Kobayashi-Hattori, K., Nakamura, T., Arai, S., Mura, K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Pages: 1329-1333
  • NAID: 10030981975
  • Peer Reviewed
• [Journal Article] Novel Angiotensin I-Converting Enzyme Inhibitory Peptides Found in a Thermolysin-Treated Elastin with Antihypertensive Activity (2012)
  • Author(s): Sato, Yuko
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 7 Pages: 1329-1333
  • DOI: 10.1271/bbb.120083
  • NAID: 10030981975
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] An unexpected [M+I]+ ion formation in phosphasilene compounds detection by electrospray ionization mass spectrometry (2011)
  • Author(s): Hongo, Y., Li, B., Suzuki, K., Nakamura, T.
  • Journal Title: Journal of Mass Spectrometry Volume: 46 Pages: 956-959
  • Peer Reviewed
• [Journal Article] Involvement of common intermediate 3-hydroxy-L-kynurenine in chromophore biosynthesis of quinomycin family antibiotics (2011)
  • Author(s): Hirose, Y., Watanabe, K., Minami, A., Nakamura, T., Oguri, H., Oikawa,H.
  • Journal Title: The Journal of Antibiotics Volume: 64 Pages: 117-122
  • NAID: 10030637815
  • Peer Reviewed
• [Journal Article] An unexpected [M+I]+ ion formation in phosphasilene compounds detection by electrospray ionization mass spectrometry (2011)
  • Author(s): go, Yayoi; Li, Baolin; Suzuki, sunori; Nakamura, Takemichi
  • Journal Title: Journal of Mass Spectrometry Volume: 46 Issue: 9 Pages: 956-959
  • DOI: 10.1002/jms.1975
  • Peer Reviewed
• [Presentation] Energy-resolved ion mobility tandem mass spectrometry - a new way for characterizing isomeric ions formed in fragmentation processes (2013)
  • Author(s): Nakamura, T., et al
  • Organizer: 24th Australian and New Zealand Society for Mass Spectrometry Conference
  • Place of Presentation: Trinity College, University of Melbourne,Melbourne,Australia(招待講演)
  • Year and Date: 2013-02-06
• [Presentation] Energy-resolved Ion Mobility Tandem Mass Spectrometry - A New Way for Characterizing Isomeric Ions Formed in Fragmentation Processes (2013)
  • Author(s): Takemichi Nakamura
  • Organizer: 24th Australian and New Zealand Society for Mass Spectrometry Conference
  • Place of Presentation: Melbourne, Australia
  • Invited
• [Presentation] Energy-resolved ion-mobility tandem mass spectrometry: a new tool for probing gas-phase isomerization and fragmentation of small molecules (2012)
  • Author(s): Nakamura, T., et al.
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: Kyoto International Conference Center, Kyoto
  • Year and Date: 2012-09-20
• [Presentation] Probing fragmentation characteristics of cyclic peptides with integrated tandem mass spectrometry approaches (2012)
  • Author(s): Nakamura, T., et al.
  • Organizer: 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver Convention Centre, Vancouver,Canada
  • Year and Date: 2012-05-23
• [Presentation] Probing fragmentation characteristics of cyclic peptides with integrated tandem mass spectrometry approaches (2012)
  • Author(s): Takemichi Nakamura
  • Organizer: 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver, Canada
• [Presentation] Energy-resolved ion-mobility tandem mass spectrometry: a new tool for probing gas-phase isomerization and fragmentation of small molecules (2012)
  • Author(s): Takemichi Nakamura
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: Kyoto, Japan
• [Presentation] フラグメンテーションの基礎とCID, ECD/ETD (2011)
  • Author(s): 中村健道
  • Organizer: 第38回BMSコンファレンス(BMS2011)
  • Place of Presentation: 箱根高原ホテル,箱根町(招待講演)
  • Year and Date: 2011-07-11
• [Presentation] フラグメンテーションの基礎とCID,ECD/ETD (2011)
  • Author(s): 中村健道
  • Organizer: 第38回BMSコンファレンス(BMS2011)
  • Place of Presentation: 箱根高原ホテル,足柄下郡箱根町(招待講演)
  • Year and Date: 2011-07-11
• [Presentation] Tandem mass spectrometric structural characterization of cyclic peptides: Revisited (2011)
  • Author(s): Nakamura, T., et al.
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Colorado Convention Center, Denver,USA
  • Year and Date: 2011-06-09
• [Presentation] Tandem mass spectrometric structural characterization of cyclic peptides : Revisited (2011)
  • Author(s): Nakamura, T., et al
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Colorado Convention Center, Denver, CO, USA
  • Year and Date: 2011-06-09
• [Book] フラグメンテーション,現代質量分析学 (2013)
  • Author(s): 中村健道
  • Publisher: 化学同人
• [Book] 現代質量分析学 (2013)
  • Author(s): 中村健道
  • Publisher: 化学同人