Identification of valuable genes from a hyperthermophile by focusing attention on its transcriptional regulation network

• Project/Area Number: 22550151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: KANAI Tamotsu 京都大学, 大学院・工学研究科, 講師 (10346083)
• Co-Investigator(Renkei-kenkyūsha): ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 超好熱菌 / アーキア / 有用遺伝子 / 転写制御因子 / EMSA / マイクロアレイ解析 / 遺伝子 / 遺伝子制御 / 始原菌

Research Abstract

Proteins from thermophiles are expected as a source of excellent biomaterials that can function under high temperature conditions, where chemical reactions are accelerated. This study deals with functional analysis on hypothetical genes of the hyperthermophilic archaeon, Thermococcus kodakarensis, with focusing attention onits transcriptional regulation network. As a result, a new sugar-degrading enzyme was identified that can hydrolyze maltooligosaccharides.

Research Products

• [Journal Article] Genetic studies on the virus-like regions in the genome of hyperthermophili (2013)
  • Author(s): Tagashira K, W. Fukuda, M. Matsubara, T.Kanai, H. Atomi, and T. Imanaka
  • Journal Title: Extremophiles Volume: vol.17 Issue: 1 Pages: 153-160
  • DOI: 10.1007/s00792-012-0504-6
  • Peer Reviewed
• [Journal Article] An archaeal histone is required for transformation of Thermococcus kodakarensis (2012)
  • Author(s): Cubonova, L., M. Katano, T. Kanai, H. Atomi, J. N. Reeve, and T. J. Santangelo
  • Journal Title: J. Bacteriol Volume: vol.194 Issue: 24 Pages: 6864-6874
  • DOI: 10.1128/jb.01523-12
  • Peer Reviewed
• [Journal Article] Distinct physiological roles of the three [NiFe]-hydrogenase orthologs in the hyperthermophilic archaeon Thermococcus kodakarensis (2011)
  • Author(s): Kanai, T., R. Matsuoka, H. Beppu, A. Nakajima, Y. Okada, H. Atomi, and T. Imanaka
  • Journal Title: J. Bacteriol. Volume: vol.193 Issue: 12 Pages: 3109-3116
  • DOI: 10.1128/jb.01072-10
  • Peer Reviewed
• [Journal Article] Structural basis for branching-enzyme activity of glycoside hydrolase family 57: structure and stability studies of a novel branching enzyme from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2011)
  • Author(s): Santos, C.R., C.C. Tonoli, D.M. Trindade, C. Betzel, H. Takata, T. Kuriki, T. Kanai, T. Imanaka, R.K. Arni, and M.T. Murakami
  • Journal Title: Proteins Volume: vol.79 Issue: 2 Pages: 547-557
  • DOI: 10.1002/prot.22902
  • Peer Reviewed
• [Journal Article] Application of hyperthermophiles and their enzymes (2011)
  • Author(s): Atomi, H., Sato, T., Kanai, T
  • Journal Title: Cur. Opi. Biotech Volume: vol.22 Issue: 5 Pages: 618-626
  • DOI: 10.1016/j.copbio.2011.06.010
  • Peer Reviewed
• [Journal Article] Structural basis for branching-enzyme activity of glycoside hydrolase family 57 : structure and stability studies of a novel branching enzyme from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2011)
  • Author(s): Santos, C.R., Tonoli, C.C.C., Trindade, D.M., Betzel, C., Takata, H., Kuriki, T., Kanai, T., Imanaka, T., Arni, R.K., Murakami, M.T.
  • Journal Title: PROTEINS : Structure, Function, and Bioinformatics Volume: 79 Pages: 547-557
  • Peer Reviewed
• [Presentation] 超好熱性アーキアの環境適応に関わる転写調節因子の解析 (2013)
  • Author(s): 金井保、武富尚吾、小谷徹、釜下知之、中之庄正弘、今中忠行、 跡見晴幸
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学川内キャンパス(招待講演)
  • Year and Date: 2013-03-27
• [Presentation] 超好熱性アーキアの環境適応に関わる転写調節因子の解析 (2013)
  • Author(s): 金井保、武富尚吾、小谷徹、釜下知之、中之庄正弘、今中忠行、跡見晴幸
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学川内キャンパス
  • Invited
• [Presentation] 超好熱性アーキアにおける熱ショック応答転写生制御因子の機能解析 (2012)
  • Author(s): 金井保、小谷徹、釜下知之、今中忠行、跡見晴幸
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-22
• [Presentation] 超好熱性アーキア Thermococcus kodakarensis ゲノムからの新規転写制御因子の同定 (2012)
  • Author(s): 山本康之、金井保、跡見晴幸
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-22
• [Presentation] 超好熱性アーキアにおける熱ショック応答転写制御因子の機能解析 (2012)
  • Author(s): 金井保、小谷徹、釜下知之、今中忠行、跡見晴幸
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 超好熱性アーキアThermococcus kodakarensisゲノムからの新規転写制御因子の同定 (2012)
  • Author(s): 山本康之、金井保、跡見晴幸
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 超好熱性アーキア Thermococcus kodakarensis における糖代謝関連転写制御因子 Tgr の機能解析 (2011)
  • Author(s): 小谷徹、金井保、跡見晴幸
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学坂本キャンパス良順会館
  • Year and Date: 2011-11-27
• [Presentation] 超好熱性アーキア Thermococcus kodakarensis のゲノム配列からの新規転写制御因子の同定 (2011)
  • Author(s): 長崎大学坂本キャンパス良順会館
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学坂本キャンパス良順会館
  • Year and Date: 2011-11-27
• [Presentation] Elucidation of the functions of the ArsR transcription regulators in Thermococcus kodakarensis (2011)
  • Author(s): 李瑞賢、 金井保、 跡見晴幸
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学坂本キャンパス良順会館
  • Year and Date: 2011-11-27
• [Presentation] 超好熱性アーキアThermococcus kodakarensisにおける糖代謝関連転写制御因子Tgrの機能解析 (2011)
  • Author(s): 小谷徹, 金井保, 跡見晴幸
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学良順会館
  • Year and Date: 2011-11-27
• [Presentation] 超好熱性アーキアThermococcus kodakarensisのゲノム配列からの新規転写制御因子の同定 (2011)
  • Author(s): 山本康之, 金井保, 跡見晴幸
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学良順会館
  • Year and Date: 2011-11-27
• [Presentation] Elucidation of the functions of the ArsR transcription regulators in Thermococcus kodakarensis (2011)
  • Author(s): S.Lee, T.Kanai, H.Atomi
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学良順会館
  • Year and Date: 2011-11-27
• [Presentation] Distinct physiological roles of the three [NiFe]-hydrogenase orthologs in the hyperthermophilic archaeon Thermococcus kodakarensis (2011)
  • Author(s): T. Kanai, R. Matsuoka, H. Beppu, A. Nakajima, Y . Okada, H. Atomi, T . Imanaka
  • Organizer: Thermophiles2011
  • Place of Presentation: Yellow Stone, USA
  • Year and Date: 2011-09-13
• [Presentation] 超好熱性アーキアのトランスクリプトーム解析 (2011)
  • Author(s): 金井保、武富尚吾、村上平、中之庄正弘、藤原伸介、跡見晴幸、今中忠行
  • Organizer: 京都大学微生物科学寄附研究部門主催シンポジウム
  • Place of Presentation: 京都大学芝蘭会館稲盛ホール
  • Year and Date: 2011-06-23
• [Presentation] Regulation mechanism of archaeal heat shock regulator (2010)
  • Author(s): T. Kanai, T . Kamashita, H. Atomi, T . Imanaka
  • Organizer: Extremophiles2010
  • Place of Presentation: Azores, Portugal
  • Year and Date: 2010-09-14
• [Presentation] Transcriptome Analysis on a Heat Shock Regulon in the Hyperthermophilic Archaeon, Thermococcus kodakaraensis (2010)
  • Author(s): T . Kanai, S. Takedomi, T. Kamashita, M.Nakanosho, S. Fujiwara, H. Atomi, T .Imanaka
  • Organizer: International Meeting of the Microbiological Society of Korea
  • Place of Presentation: Hanwha Resort hotel, Uljin, Korea (Invited)
  • Year and Date: 2010-05-07
• [Presentation] Transcriptome Analysis on a Heat Shock Regulon in the Hyperthermophilic Archaeon, Thermococcus kodakaraensis (2010)
  • Author(s): T.Kanai, S.Takedomi, T.Kamashita, M.Nakanosho, S.Fujiwara, H.Atomi, T.Imanaka
  • Organizer: International Meeting of the Microbiological Society of Korea
  • Place of Presentation: Hanwha Resort hotel, Uljin, Korea(招待講演)
  • Year and Date: 2010-05-07
• [Book] Enzymes from thermophilic organisms (2013)
  • Author(s): T. Kanai and H. Atomi, Wiley-VCH
  • Publisher: Protein Engineering Handbook
• [Book] Protein Engineering Handbook, vol.3 chap.7 (2013)
  • Author(s): Kanai, T., H. Atomi
  • Total Pages: 18
  • Publisher: Wiley-VCH
• [Remarks]
  • URL: http://www.sbchem.kyoto-u.ac.jp/atomi-lab/
• [Remarks]
  • URL: http://www.sbchem.kyoto-u.ac.jp/atomi-lab/
• [Remarks]
  • URL: http://www.sbchem.kyoto-u.ac.jp/atomi-lab/