| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Research Abstract |
In this project, we successful synthesized a fluorometric potassiumdetecting reagent, PSO-5, consisted of thrombin binding aptamer (TBA) carrying FAM at the 5'-end conjugated with a peptide carrying TAMRA and biotin at the middle and at its C-terminus, respectively. K^+ in the cell was visualized based on the FRET ratio of the ternary complex of streptavidin with PSO-5 and biotinylated nuclear export signal peptide. Change of FRET signal of PSO-5 was observed that indicated a decrease in K^+ concentration in the cell upon addition of apoptosis-inducing drug to monitor K^+ efflux from the cell.
|
