Controlled intracellular delivery of Multi-functional siRNA and gene silencing
| Project/Area Number |
22550159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生体機能関連化学 / siRNA / マルチコンジュゲート / 細胞質局在化 / 遺伝子サイレンシング / パプチド遺伝子導入剤 / ヌクレアーゼ耐性 / ペプチド遺伝子導入剤 |
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| Research Abstract |
The expression of bcr/abl gene in K562 cells were suppressed to 8.3% at 200nM and 11.6 % at 50nM by siRNA-NES conjugates synthesized solid phase fragment coupling. It was also found that the complex of siRNA and novel peptides (Pfect) was taken up into cells with comparable efficiency to commercially available lipofection reagent. The expression of bcr/abl gene was suppressed to 0.5% by the complex of siRNA-NES conjugate and Pfect. The complex of siRNA and Pfect showed no cyto-toxicity and an intensive resistance against nucleases digestion to extend the half-life time over 48 hours.
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Report
(4 results)
Research Products
(73 results)
