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Three dimensional observation and modeling of microorganism motion near a surface

Research Project

Project/Area Number 22560168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fluid engineering
Research InstitutionTottori University

Principal Investigator

GOTO Tomonobu  鳥取大学, 大学院・工学研究科, 教授 (00260654)

Co-Investigator(Kenkyū-buntansha) NAKAI Tonau  鳥取大学, 大学院・工学研究科, 助教 (80452548)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords微生物運動 / 流体工学 / 3次元観察 / 数値解析 / 3次元観察 / 表面近傍 / 細菌運動 / 座標取得 / 2方向同時観察 / 蛍光観察 / べん毛
Research Abstract

Swimming motion of microorganisms near a surface has close connection with the environment of lakes, marshes, oceans, or in vivo, in conjunction with the biofilm formation. The objective of this study is to make a model describing bacterial motion near a surface; approaching the surface and accumulation on the surface. We have performed bidirectional simultaneous microscopy using two synchronized high-speed cameras, and obtained simultaneously the position and posture of a bacterial cell near a surface. We have developed component technologies such as automatic measurement by the image processing, the automatic control of the microscope stage to shorten data acquisition time.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (27 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Bacterial Locomotion in an Infinite Liquid Medium and in the Presence of a Nearby Surface2013

    • Author(s)
      Goto Tomonobu, Nakai Tonau
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: Vol.3No.1 Pages: 2-7

    • NAID

      130003375373

    • URL

      https://www.jstage.jst.go.jp/article/jabmech/3/1/3_2/_pdf

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial Locomotion in an Infinite Liquid Medium and in the Presence of a Nearby Surface2013

    • Author(s)
      Tomonobu Goto, Tonau Nakai
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: 3 Pages: 2-7

    • NAID

      130003375373

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細菌の泳ぎと流体力学2012

    • Author(s)
      後藤知伸,中井唱
    • Journal Title

      油空圧技術

      Volume: Vol.51No.7 Pages: 38-44

    • Related Report
      2012 Final Research Report
  • [Journal Article] 細菌の泳ぎと流体力学2012

    • Author(s)
      後藤知伸,中井唱
    • Journal Title

      油空圧技術

      Volume: 51 Pages: 38-44

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 2方向同時観察による三次元顕微鏡システムの開発2011

    • Author(s)
      中井唱, 後藤知伸
    • Journal Title

      ケミカルエンジニヤリング

      Volume: 第56巻第1号 Pages: 11-15

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Journal Article] Boundary Element Analysis on the Fluid-Dynamic Interaction between a Singly Flagellated Bacterium and a Free Surface2010

    • Author(s)
      Goto Tomonobu, Maiguma Takafumi, Nakai Tonau
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: Vol.1No.1 Pages: 39-44

    • NAID

      130000428316

    • URL

      https://www.jstage.jst.go.jp/article/jabmech/1/1/1_1_39/_pdf

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Boundary Element Analysis on Transition of Distance and Attitude of a Bacterium near a Rigid Surface2010

    • Author(s)
      T.Goto, T.Nakai, K.Aoki
    • Journal Title

      Journal of Biomechanical Science and Engineering

      Volume: 5-4 Pages: 329-339

    • NAID

      130000252231

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Boundary Element Analysis on the Fluid-Dynamic Interaction between a Singly Flagellated Bacterium and a Free Surface2010

    • Author(s)
      T.Goto, T.Maiguma, T. Nakai
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: 1 Pages: 39-44

    • NAID

      130000428316

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 固体表面近くの細菌遊泳運動のメカニズム2010

    • Author(s)
      後藤知伸
    • Journal Title

      バイオメカニズム学会誌

      Volume: 34-3 Pages: 183-188

    • NAID

      10030699925

    • Related Report
      2010 Annual Research Report
  • [Presentation] らせん型べん毛により推進する細菌の推進効率に関する数値解析2013

    • Author(s)
      三宅正芳,中井唱,後藤知伸
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      つくば
    • Year and Date
      2013-01-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 二方向同時観察法を用いた三次元自動追跡機能付き顕微鏡の試作(二次元平面内でのリアルタイム自動追跡)2012

    • Author(s)
      植木裕太,中井 唱,後藤知伸
    • Organizer
      日本機械学会 2012年度年次大会
    • Place of Presentation
      金沢
    • Year and Date
      2012-09-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Boundary Element Analysis and Three-Dimensional Observation of Propulsive Force and Torque of a Dinoflagellate Symbiodinium2012

    • Author(s)
      Nakai Tonau, Shihira-Ishikawa Ikuko, Miyawaki Atsushi, Goto Tomonobu
    • Organizer
      The Fifth International Symposium on Aero Aqua Bio-Mechanisms
    • Place of Presentation
      台北
    • Year and Date
      2012-08-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Bacterial Locomotion in an Infinite Liquid Medium and in the Presence of a Nearby Surface2012

    • Author(s)
      Goto Tomonobu, Nakai Tonau
    • Organizer
      The Fifth International Symposium on Aero Aqua Bio-Mechanisms
    • Place of Presentation
      台北
    • Year and Date
      2012-08-26
    • Related Report
      2012 Final Research Report
  • [Presentation] BOUNDARY ELEMENT ANALYSIS OF PROPULSIVE FORCE AND TORQUE OF A DINOFLAGELLATE SYMBIODINIUM2012

    • Author(s)
      Nakai Tonau, Shihira-Ishikawa Ikuko, Miyawaki Atsushi, Goto Tomonobu
    • Organizer
      The 23rd International Congress of Theoretical and Applied Mechanics (ICTAM2012)
    • Place of Presentation
      北京
    • Year and Date
      2012-08-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 推進するらせん型の形状最適化に関するストークス流れの数値解析2012

    • Author(s)
      三宅正芳,笠原浩平,後藤知伸,中井唱
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2012
    • Place of Presentation
      浜松
    • Year and Date
      2012-05-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 2方向の明視野顕微鏡像を用いた細菌の3次元位置及び姿勢の自動推定2012

    • Author(s)
      三上和博,後藤知伸,中井唱
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2012
    • Place of Presentation
      浜松
    • Year and Date
      2012-05-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 界面近傍での細菌遊泳運動とべん毛変形の観察2012

    • Author(s)
      後藤知伸,中井唱,小谷鮎美
    • Organizer
      日本機械学会 第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪
    • Year and Date
      2012-01-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 渦鞭毛藻Symbiodinium の推進時に起きる自転運動の三次元解析2012

    • Author(s)
      中井唱,村野ひとみ, 後藤知伸
    • Organizer
      日本機械学会 第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪
    • Year and Date
      2012-01-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 同期した2 つの映像から細菌の妥当な位置及び姿勢を算出するプログラムの開発2012

    • Author(s)
      三上和博, 中井唱,後藤知伸
    • Organizer
      日本機械学会 第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪
    • Year and Date
      2012-01-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 界面近傍での細菌遊泳運動とべん毛変形の観察2012

    • Author(s)
      後藤知伸, 中井唱, 小谷鮎美
    • Organizer
      日本機械学会第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 渦鞭毛藻Symbiodiniumの推進時に起きる自転運動の三次元解析2012

    • Author(s)
      中井唱, 村野ひとみ, 後藤知伸
    • Organizer
      日本機械学会第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 同期した2つの映像から細菌の妥当な位置及び姿勢を算出するプログラムの開発2012

    • Author(s)
      三上和博, 中井唱, 後藤知伸
    • Organizer
      日本機械学会第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 渦鞭毛藻Symbiodiniumの自転によるトルクの2方向同時観察による解析2011

    • Author(s)
      中井 唱, 村野ひとみ, 後藤知伸
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2011
    • Place of Presentation
      岡山
    • Year and Date
      2011-05-28
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 単毛性細菌と境界の相互作用による形状及び遊泳運動の変化2011

    • Author(s)
      井上拓哉,中井 唱,後藤知伸
    • Organizer
      日本機械学会 中国四国支部第49期総会・講演会
    • Place of Presentation
      岡山
    • Year and Date
      2011-03-05
    • Related Report
      2012 Final Research Report
  • [Presentation] らせん型の推進効率の形状依存性に関する境界要素解析2010

    • Author(s)
      笠原浩平,中井 唱,後藤知伸
    • Organizer
      日本機械学会 2010 年度年次大会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 細菌の遊泳軌跡の曲率と境界からの距離との関係ピントはずれを利用した観察2010

    • Author(s)
      井上拓哉,中井 唱,後藤知伸
    • Organizer
      日本機械学会 2010 年度年次大会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Three Dimensional Observation of Bacterial Swimming Motion near a Surface2010

    • Author(s)
      Goto Tomonobu, Katayose Ryo, Inoue Takuya, Nakai Tonau
    • Organizer
      6th World Congress of Biomechanics
    • Place of Presentation
      Singapore
    • Year and Date
      2010-08-03
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Boundary Element Analysis of Propulsion of a Dinoflagellate Symbiodinium Generated by a Transverse Flagellum with a Membrane2010

    • Author(s)
      Nakai Tonau, Shihira-Ishikawa Ikuko, Miyawaki Atsushi, Goto Tomonobu
    • Organizer
      6th World Congress of Biomechanics
    • Place of Presentation
      Singapore
    • Year and Date
      2010-08-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 境界近傍での単毛性細菌の位置・姿勢の同時計測2010

    • Author(s)
      片寄 良,井上拓哉,後藤知伸,中井 唱
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2010
    • Place of Presentation
      旭川
    • Year and Date
      2010-06-16
    • Related Report
      2012 Final Research Report
  • [Presentation] らせん型べん毛により推進する細菌の推進効率に関する数値解析

    • Author(s)
      三宅正芳,中井唱,後藤知伸
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      産総研つくばセンター(つくば)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 方向同時観察法を用いた三次元自動追跡機能付き顕微鏡の試作(二次元平面内でのリアルタイム自動追跡)

    • Author(s)
      植木裕太,中井 唱,後藤知伸
    • Organizer
      日本機械学会 2012年度年次大会
    • Place of Presentation
      金沢大学(金沢)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Boundary Element Analysis and Three-Dimensional Observation of Propulsive Force and Torque of a Dinoflagellate Symbiodinium

    • Author(s)
      Tonau Nakai, Ikuko Shihira-Ishikawa, Atsushi Miyawaki, Tomonobu Goto
    • Organizer
      The Fifth International Symposium on Aero Aqua Bio-Mechanisms
    • Place of Presentation
      台北(台湾)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Bacterial Locomotion in an Infinite Liquid Medium and in the Presence of a Nearby Surface

    • Author(s)
      Tomonobu Goto, Tonau Nakai
    • Organizer
      The Fifth International Symposium on Aero Aqua Bio-Mechanisms
    • Place of Presentation
      台北(台湾)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] BOUNDARY ELEMENT ANALYSIS OF PROPULSIVE FORCE AND TORQUE OF A DINOFLAGELLATE SYMBIODINIUM

    • Author(s)
      Tonau Nakai, Ikuko Shihira-Ishikawa, Atsushi Miyawaki, Tomonobu Goto
    • Organizer
      The 23rd International Congress of Theoretical and Applied Mechanics (ICTAM2012)
    • Place of Presentation
      北京(中国)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 推進するらせん型の形状最適化に関するストークス流れの数値解析

    • Author(s)
      三宅正芳,笠原浩平,後藤知伸,中井唱
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2012
    • Place of Presentation
      ACT CITY Hamamatsu(浜松)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2方向の明視野顕微鏡像を用いた細菌の3次元位置及び姿勢の自動推定

    • Author(s)
      三上和博,後藤知伸,中井唱
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2012
    • Place of Presentation
      ACT CITY Hamamatsu(浜松)
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.damp.tottori-u.ac.jp/~lab5/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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