| Project/Area Number |
22560771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
|
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| Research Institution | Chubu University (2011-2012)
Okayama University (2010) |
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Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Koreyoshi 岡山大学, 自然科学研究科, 教授 (70294436)
IMANAKA Hiroyuki 岡山大学, 自然科学研究科, 助教 (10379711)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 酵素 / 配向制御固定化 / 親和性ペプチドタグ / シリカ / ポリスチレン / 配向制御固定化法 / βーガラクトシダーゼ / Bacillus circulans / 構造制御固定化法 / 固定化酵素 / シリカゲル / 表面特異的親和性ペプチド / 配向制御 / GST |
|---|
| Research Abstract |
In this study, a method to immobilize enzymes/proteins on the solid surface without suffering from denaturation that might occur upon coming in contact with the solid surface was investigated. Conjugating peptide tags that show a high affinity to silica and polystyrene surfaces, toenzymes enabled a strong, nearly irreversible immobilization without appreciable loss of activity.
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