| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
We established procedures through which RNA viruses, EMCV and HEV are synthesized in vitro. We synthesized EMCV in the human cell extract-derived in vitro protein expression system from DNA, and purified it for analysis with a transmission electron microscope. HEV capsid proteins were produced in E. coli and purified. The purified HEV proteins were incubated in vitro for the formation of HEV viral capsules. To apply these viral particles to drug delivery and bio-imaging, the amino acid sequences of the capsid proteins of EMCV and HEV were altered.
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