In vitro-synthesis of highly pure viral particles and their application to nanotechnology
| Project/Area Number |
22560774
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | University of Hyogo |
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Principal Investigator |
IMATAKA Hiroaki 兵庫県立大学, 大学院・工学研究科, 教授 (50201942)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ウイルス / ウイルス粒子 / 試験管内合成 / セルフリー / カプシド / ナノテクノロジー / タンパク質合成 / セルフリーシステム / 脳心筋炎ウイルス / ドラッグデリバリー / Ｅ型肝炎ウイルス / バイオイメージング / RNAウイルス / 人工ウイルス / 再構成 / ナノマテリアル / ウィルス粒子 |
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| Research Abstract |
We established procedures through which RNA viruses, EMCV and HEV are synthesized in vitro. We synthesized EMCV in the human cell extract-derived in vitro protein expression system from DNA, and purified it for analysis with a transmission electron microscope. HEV capsid proteins were produced in E. coli and purified. The purified HEV proteins were incubated in vitro for the formation of HEV viral capsules. To apply these viral particles to drug delivery and bio-imaging, the amino acid sequences of the capsid proteins of EMCV and HEV were altered.
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Report
(4 results)
Research Products
(49 results)
