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Re-evaluation of microbial methane stable carbon isotope frac-tionation under high hydrostatic pressure

Research Project

Project/Area Number 22560811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Earth system and resources enginnering
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

TASUMI Eiji  独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 技術研究副主任 (50553228)

Co-Investigator(Kenkyū-buntansha) IMACHI Hiroyuki  独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (20361933)
TAKAI Ken  独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクター (80359166)
KAWAGUCHI Shinsuke  独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 研究員 (50553088)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsメタン / 圧力培養 / メタン炭素同位体 / 資源探査
Research Abstract

Methanosarcina sp. NT-MS1 that had been isolated from Nankai Trough subseafloor sediment using batch-culture was cultured under high pressure conditions. Its optimum pressure for growth was 15 MPa, and it showed positive growth with methanogenesis at its habitat pressure (25 MPa). Takai et al. reported that the hyperthermophilic methanogen, Methanopyrus kandleri strain 116, generates “isotopically heavy” methane under high pressure conditions. But, strain NT-MS1 generated “isotopically light (Δ~<13>C-CH_4 = -81~-63‰)” methane at 5 to 35 Mpa.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] 深海底環境からのメタン生成菌の効率的な培養技術2012

    • Author(s)
      井町寛之、稲垣史生
    • Journal Title

      月刊ファインケミカル, シーエムシー出版

      Volume: 11月号

    • Related Report
      2012 Final Research Report
  • [Journal Article] Cultivation of methanogenic community from subseafloor sediments using a con-tinuous flow bioreactor2011

    • Author(s)
      Imachi H, Aoi K, Tasumi E, Saito Y, Yamanaka Y, Saito Y, Yamaguchi T, Tomaru H, Takeuchi R, Morono Y, Inagaki F and Takai K
    • Journal Title

      ISME J

      Volume: 5 Pages: 1913-1925

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 東北地方太平洋沖地震後に観察された深海底堆積物中メタンの多様性が示すものとは2012

    • Author(s)
      田角栄二、布浦拓郎、川口慎介、今野祐太、藤倉克則、古島靖夫、渡部裕美、宮本教生、辻健、新井和乃、野口拓郎、長谷川和範、土岐知弘、野牧秀隆、谷川亘、笠谷貴史、砂村倫成、高井研
    • Organizer
      第28回日本微生物生態学会
    • Place of Presentation
      豊橋技術科学大学
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 東北地方太平洋沖地震後に観察された深海底堆積物中メタンの多様性が示すものとは2012

    • Author(s)
      田角 栄二
    • Organizer
      第28回日本微生物生態学会
    • Place of Presentation
      豊橋技術科学大学(豊橋市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 深海堆積物から分離したメタン生成アーキアの菌学的特徴とその圧力応答2010

    • Author(s)
      田角栄二
    • Organizer
      第26回日本微生物生態学会大会
    • Place of Presentation
      筑波大学(茨城県)
    • Year and Date
      2010-11-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 深海底堆積物から分離したメタン生成アーキアの菌学的特徴とその圧力応答2010

    • Author(s)
      田角栄二、吉田奈央子、片山新太、江原真之、井町寛之、高井研
    • Organizer
      第26回日本微生物生態学会
    • Place of Presentation
      筑波大学
    • Year and Date
      2010-11-24
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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